{"id":100492,"date":"2018-03-11T10:22:02","date_gmt":"2018-03-11T10:22:02","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/development-of-in-vitro-and-in-vivo-bioreactors-for-bone-tissue-engineering\/"},"modified":"2018-03-11T10:22:02","modified_gmt":"2018-03-11T10:22:02","slug":"development-of-in-vitro-and-in-vivo-bioreactors-for-bone-tissue-engineering","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/cultivo-de-tejidos\/development-of-in-vitro-and-in-vivo-bioreactors-for-bone-tissue-engineering\/","title":{"rendered":"Development of in vitro and in vivo bioreactors for bone tissue engineering"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Martin Andreas Koch <\/strong><\/h2>\n<p>Large bone defects constitute a challenge for the clinical field, because they cannot be repaired by the  body itself, but require the implantation of suitable bone grafts. To overcome the drawbacks of grafts from  autologous or allogous sources, modern bone tissue engineering aims to replace lost tissue by cultivating  cells in vitro on porous biomaterials. The cell culture on large porous scaffolds has shown to be difficult,  requiring bioreactors, which are used for tissue culture and the study of cell behaviour in 3d scaffolds. Of  special interest is the mechanical conditioning of the cultured tissue for bioreactor-based bone tissue  engineering, which is able to enhance the osteogenic potential of the synthetic grafts.    in this work two bioreactor systems were developed to allow insight into bioactive properties of different  scaffold materials and the mechanoregulation of cell or tissue behaviour. An in vitro perfusion bioreactor  system was developed for the cell seeding and culture on porous biomaterial cylinders. Several studies for  the determination of applicable cell seeding parameters were conducted, as well as experiments of cell  culture under steady fluid flow with additional mechanical stimulation by alternating fluid flow. A bone  chamber system was developed as an in vivo bioreactor. The system produced a large bone defect in dog  tibia and allowed the repeated implantation of large porous scaffolds of different material compositions.  the ingrowing tissue was observed to allow conclusions about osteoconductive or osteinductive  properties of the scaffolds. Additionally a compression device was developed to apply cyclic loading on  the scaffolds in vivo to study the effect of mechanical stimulation on tissue development.    the studies with the developed in vitro perfusion bioreactor system have shown that it is possible to seed  cells throughout large porous scaffolds, which is deemed crucial for the further cell culture. The long time  cell culture showed the proliferation of mesenchymal stem cells up to two weeks. The stimulation pattern  used in the study enhanced the expression of osteocalcin, indicating an enhanced cell activity, but the  absence of runx2 and collagen i expression rendered the determination of differentiation inconclusive.  the developed bone chamber system proved to be functional in the surgical environment during the in  vivo experiments. Occurring complications during the experiments did not allow the application of the  cyclic loading of implanted scaffolds. Delayed bone formation due to created bone defect and remaining  scaffold material did not allow final conclusions about the scaffold material properties. Nevertheless the  study provides input for further development of the device and clinical protocol.    the conducted studies constitute a novelty regarding the creation of bioreactors for the study of synthetic  porous scaffolds of large dimensions in vitro and in vivo. The developed systems form the basis for further  studies in mechanobiology of bone cells and tissue.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Development of in vitro and in vivo bioreactors for bone tissue engineering<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Development of in vitro and in vivo bioreactors for bone tissue engineering <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Martin Andreas Koch <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de catalunya<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 23\/04\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Damien Lacroix<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: josep antoni Planell estany <\/li>\n<li>elisabet Engel l\u00f3pez (vocal)<\/li>\n<li>melba Navarro toro (vocal)<\/li>\n<li>paulo Antonio Netti (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Martin Andreas Koch Large bone defects constitute a challenge for the clinical field, because they cannot be 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