{"id":100576,"date":"2018-03-11T10:22:07","date_gmt":"2018-03-11T10:22:07","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudi-de-la-regulacio-transcripcional-del-gen-de-cd69\/"},"modified":"2018-03-11T10:22:07","modified_gmt":"2018-03-11T10:22:07","slug":"estudi-de-la-regulacio-transcripcional-del-gen-de-cd69","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/barcelona\/estudi-de-la-regulacio-transcripcional-del-gen-de-cd69\/","title":{"rendered":"Estudi de la regulaci\u00f3 transcripcional del gen de cd69"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Berta Vazquez Prat <\/strong><\/h2>\n<p>Cd69 es una lectina tipo-c con orientaci\u00f3n tipo ii que participa en la migraci\u00f3n de linfocitos y la secreci\u00f3n de citocinas. La expresi\u00f3n de cd69 es uno de los indicadores m\u00e1s tempranos disponible de la activaci\u00f3n de los leucocitos y su r\u00e1pida inducci\u00f3n se produce a trav\u00e9s de la activaci\u00f3n de la transcripci\u00f3n. En esta tesis se ha examinado el mecanismo molecular subyacente a la regulaci\u00f3n transcripcional del gen de cd69 de rat\u00f3n in vivo en c\u00e9lulas t y b. El an\u00e1lisis de la regi\u00f3n 45kb por encima del gen cd69 revel\u00f3 conservaci\u00f3n evolutiva en el promotor y en cuatro secuencias no codificantes (cns) que fueron llamados cns1, cns2, cns3 y cns4. Se encontraron regiones de hipersensibilidad en experimentos de hipersensibilidad a la digesti\u00f3n por dnasa i y ensayos de inmunoprecipitaci\u00f3n de cromatina mostraron modificaciones epigen\u00e9ticas espec\u00edfica.En ensayos de transfecci\u00f3n transitoria en c\u00e9lulas t, mostraban que cns2 y cns4 ten\u00edan una actividad enhancer constitutiva e inducible. Para testar la funcion de los diferentes cns se hicieron ratones transg\u00e9nicos y se encontr\u00f3 que el promotor confer\u00eda una expresi\u00f3n regulada durante la selecci\u00f3n positiva de los timocitos, pero no pod\u00eda apoyar una expresi\u00f3n regulada en linfocitos maduros. La inclusi\u00f3n de cns1 y cns2 caus\u00f3 la supresi\u00f3n de la expresi\u00f3n de cd69 i la adici\u00f3n de cns3 y cns4 dio lugar a una expresi\u00f3n regulada las c\u00e9lulas t, pero no en las c\u00e9lulas b. Concluimos que cns1-4  son elementos reguladores importantes que interact\u00faan tanto positiva como negativamente con el promotor de cd69 y que contribuyen de manera diferente a la expresi\u00f3n cd69 en las c\u00e9lulas t y b.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudi de la regulaci\u00f3 transcripcional del gen de cd69<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudi de la regulaci\u00f3 transcripcional del gen de cd69 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Berta Vazquez Prat <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 29\/04\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Pilar Lauzurica G\u00f3mez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: manuel Lopez cabrera <\/li>\n<li>luciano Di croce (vocal)<\/li>\n<li>  (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Berta Vazquez Prat Cd69 es una lectina tipo-c con orientaci\u00f3n tipo ii que participa en la migraci\u00f3n [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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