{"id":103610,"date":"2018-03-11T10:26:17","date_gmt":"2018-03-11T10:26:17","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-molecular-del-cancer-de-tiroides-experimental\/"},"modified":"2018-03-11T10:26:17","modified_gmt":"2018-03-11T10:26:17","slug":"estudio-molecular-del-cancer-de-tiroides-experimental","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sevilla\/estudio-molecular-del-cancer-de-tiroides-experimental\/","title":{"rendered":"Estudio molecular del c\u00e1ncer de tiroides experimental"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Mar\u00eda In\u00e9s Carmona  L\u00f3pez <\/strong><\/h2>\n<p>En el presente trabajo se estudiaron patrones histol\u00f3gicos y alteraciones biomoleculares de tumores tiroideos experimentales producidos en ratas mediante al administraci\u00f3n de un factor boci\u00f3geno. Se relacionaron los tipos histol\u00f3gicos obtenidos en el modelo con mutaciones en los genes n-ras, b-raf y pi3kca, descritos generalmente en humanos. Se analiz\u00f3 el tejido tumoral parafinado de ratas wistar tratadas con perclorato pot\u00e1sico (kclo4) al 1%, en agua de bebida ad limitum, durante 18 meses. El experimento se dise\u00f1\u00f3 con tres subgrupos de 10 tiroides: subgrupo i (control) constituido por gl\u00e1ndulas procedentes de animales testigo (ambiente natural); subgrupo ii y iii (experimentales) constituidos por tiroides de animales sometidos a las condiciones del experimento pero que, respectivamente, presentan tumores que no producen met\u00e1stasis pero cuya malignidad se establece por la presencia de invasi\u00f3n capsular y\/o vascular, y tumores afirmativamente malignos con met\u00e1stasis a distancia. El estudio histol\u00f3gico se llev\u00f3 a cabo sobre cortes tisulares te\u00f1idos con hematoxilina- eosina y sometidos a inmunorreacci\u00f3n para tiroglubulina (tg) y cd34. La extracci\u00f3n del adn del tejido parafinado se realiz\u00f3 mediante el m\u00e9todo del fenol-cloroformo-proteinasa k. Teniendo en cuenta las alteraciones gen\u00e9ticas m\u00e1s comunes en el c\u00e1ncer tiroideo humano se amplific\u00f3 y secuenci\u00f3 el ex\u00f3n 1 del gen n-ras, el ex\u00f3n 15 del gen b-raf y los exones 9 y 20 del gen pi3kca.Todos los tumores corresponden al tipo folicular (formaci\u00f3n de fol\u00edculos y n\u00facleos celulares densos), si bien desarrollan estructuras pseudopapilares por protrusi\u00f3n de la proliferaci\u00f3n tisular en la luz de grandes espacios vasculares sangu\u00edneos. Ninguno de los 20 tumores tiroideos mostr\u00f3 las alteraciones gen\u00e9ticas esperadas, propias de tumores humanos, en los genes estudiados, pero en todos ellos se identificaron cuatro mutaciones en el gen b-raf, tres de ellas silenciosas y la cuarta responsable de un cambio de amino\u00e1cido en la prote\u00edna. La producci\u00f3n de tumores tiroideos en ratas mediante la exclusiva administraci\u00f3n de kclo4 es un modelo experimental de f\u00e1cil manejo para el estudio de equiValencias y \u00fatil para el mejor conocimiento de la carcinog\u00e9nesis y para una mejor interpretaci\u00f3n de la patolog\u00eda tumoral de la gl\u00e1ndula. El an\u00e1lisis biomolecular sugiere que alteraciones del gen n-ras o del pi3kca pueden no estar involucradas en la carcinog\u00e9nesis de tumores tiroideos inducidos por el procedimiento experimental aplicado. No obstante, la mutaci\u00f3n encontrada y descrita por primera vez en el gen b-raf puede estar implicada en el proceso.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio molecular del c\u00e1ncer de tiroides experimental<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio molecular del c\u00e1ncer de tiroides experimental <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Mar\u00eda In\u00e9s Carmona  L\u00f3pez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Sevilla<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/09\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Manuel De Miguel Rodr\u00edguez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ricardo Gonzalez campora <\/li>\n<li>concepcion Morales jimenez (vocal)<\/li>\n<li>Juan  Jos\u00e9 Sanchez carrillo (vocal)<\/li>\n<li>Antonio L\u00f3pez beltr\u00e1n (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mar\u00eda In\u00e9s Carmona L\u00f3pez En el presente trabajo se estudiaron patrones histol\u00f3gicos y alteraciones biomoleculares de tumores [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483,10715],"tags":[7892,11084,26540,209739,209738,11092],"class_list":["post-103610","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","category-sevilla","tag-antonio-lopez-beltran","tag-concepcion-morales-jimenez","tag-juan-jose-sanchez-carrillo","tag-manuel-de-miguel-rodriguez","tag-maria-ines-carmona-lopez","tag-ricardo-gonzalez-campora"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/103610","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=103610"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/103610\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=103610"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=103610"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=103610"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}