{"id":104441,"date":"2018-03-11T10:27:24","date_gmt":"2018-03-11T10:27:24","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/structures-studies-on-e-coli-dna-polymerase-iii-clamp-loader-subcomplexes\/"},"modified":"2018-03-11T10:27:24","modified_gmt":"2018-03-11T10:27:24","slug":"structures-studies-on-e-coli-dna-polymerase-iii-clamp-loader-subcomplexes","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-molecular\/structures-studies-on-e-coli-dna-polymerase-iii-clamp-loader-subcomplexes\/","title":{"rendered":"Structures studies on e.coli dna polymerase iii clamp loader subcomplexes."},"content":{"rendered":"

Tesis doctoral de Marta Rajkiewicz <\/strong><\/h2>\n

A crucial step in dna replication is opening of the ring-shaped sliding clamp, and its proper placement on the dna template. This process is carried out by the clamp loader complexes, in an atp dependent reaction that is stimulated by the primed dna. In the gram-negative bacteria e. Coli, two main components of the clamp loader are \u00c2\u00bf and \u00c2\u00bf, products of the same dnax gene, that isolated in solution, form homotetramers. In the presence of additional subunits \u00c2\u00bf and \u00c2\u00bf\u00c2\u00bf, the equilibrium shifts and the (dnax)3\u00c2\u00bf\u00c2\u00bf\u00c2\u00bf is formed. Other two subunits, \u00c2\u00bf and \u00c2\u00bf , form a complex with the clamp loader. The \u00c2\u00bf subunit binds to the dnax proteins (\u00c2\u00bf or \u00c2\u00bf) on one side and to \u00c2\u00bf on the other. The \u00c2\u00bf subunit forms a bridge between (dnax)3\u00c2\u00bf\u00c2\u00bf\u00c2\u00bfand \u00c2\u00bf links the clam loader to ssb facilitating the displacement of primase from newly synthesized rna primer, thus playing a crucial role in okazaki fragment synthesis. we have analyzed the structure of e. Coli \u00c2\u00bf4 and \u00c2\u00bf4\u00c2\u00bf\u00c2\u00bf complexes using three-dimensional electron microscopy. Reaching convergence in 3d map refinement was hindered by several reasons: the small size (165 kda to 222 kda), lack of symmetry, and globular shape of the complexes, which resulted in low signal in the images; and large structural heterogeneity, which could be caused by intrinsic complex flexibility, and was refractory even to sample cross-linking procedures. Nevertheless, by using maximum-likelihood image classification methods we were able to select homogenous subpopulations of particles and calculate consensus three-dimensional maps for all complexes. Combination of our maps with crystallographic or homology model structures show how dnax proteins can establish a large variety of interactions among them, in agreement with their versatility in forming complexes with different accessory proteins during polymerase holoenzyme assembly. Difference maps show the location of the \u00c2\u00bf\u00c2\u00bf heterodimer in the \u00c2\u00bf4\u00c2\u00bf\u00c2\u00bf complex, and how the interactions among \u00c2\u00bf subunits are altered by its presence.<\/p>\n

 <\/p>\n

Datos acad\u00e9micos de la tesis doctoral \u00abStructures studies on e.coli dna polymerase iii clamp loader subcomplexes.<\/strong>\u00ab<\/h3>\n
    \n
  • T\u00edtulo de la tesis:<\/strong>\u00a0 Structures studies on e.coli dna polymerase iii clamp loader subcomplexes. <\/li>\n
  • Autor:<\/strong>\u00a0 Marta Rajkiewicz <\/li>\n
  • Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n
  • Fecha de lectura de la tesis:<\/strong>\u00a0 22\/10\/2010<\/li>\n<\/ul>\n

     <\/p>\n

    Direcci\u00f3n y tribunal<\/h3>\n
      \n
    • Director de la tesis<\/strong>\n
        \n
      • Jos\u00e9 Mar\u00eda Carazo Garcia<\/li>\n<\/ul>\n<\/li>\n
      • Tribunal<\/strong>\n
          \n
        • Presidente del tribunal: jose Lopez carrascosa <\/li>\n
        • oscar Antonio Llorca blanco (vocal)<\/li>\n
        • Rafael N\u00fa\u00f1ez ram\u00edrez (vocal)<\/li>\n
        • mikel Valle rodriguez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          Tesis doctoral de Marta Rajkiewicz A crucial step in dna replication is opening of the ring-shaped sliding clamp, and its […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483],"tags":[21426,14351,211087,96955,211088,169248],"class_list":["post-104441","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","tag-jose-lopez-carrascosa","tag-jose-maria-carazo-garcia","tag-marta-rajkiewicz","tag-mikel-valle-rodriguez","tag-oscar-antonio-llorca-blanco","tag-rafael-nunez-ramirez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/104441","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=104441"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/104441\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=104441"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=104441"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=104441"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}