{"id":104496,"date":"2018-03-11T10:27:29","date_gmt":"2018-03-11T10:27:29","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/electrochemical-dna-microbiosensor-based-on-a-three-electrode-configuration-for-the-detection-of-specific-dna-sequences\/"},"modified":"2018-03-11T10:27:29","modified_gmt":"2018-03-11T10:27:29","slug":"electrochemical-dna-microbiosensor-based-on-a-three-electrode-configuration-for-the-detection-of-specific-dna-sequences","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/electroquimica\/electrochemical-dna-microbiosensor-based-on-a-three-electrode-configuration-for-the-detection-of-specific-dna-sequences\/","title":{"rendered":"Electrochemical dna microbiosensor based on a three-electrode configuration for the detection of specific dna sequences"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Larraitz A\u00f1orga G\u00f3mez <\/strong><\/h2>\n<p>En este documento se presenta un microbiosensor electroqu\u00edmico para la detecci\u00f3n de secuencias espec\u00edficas de adn. bas\u00e1ndose en la compatibilidad de los m\u00e9todos electroqu\u00edmicos con las tecnolog\u00edas de microfabricaci\u00f3n, se ha desarrollado un microdispositivo que integra el sistema de tres electrodos necesario para la llevar a cabo las medidas electroqu\u00edmicas. El electrodo de trabajo de oro, el contraelectrodo de platino y el electrodo de referencia de plata son pel\u00edculas delgadas fabricadas por sputtering e integradas en el dispositivo. Se han optimizado diferentes par\u00e1metros del proceso de fabricaci\u00f3n, lo que permite la selecci\u00f3n del m\u00e1s apropiado para el desarrollo del biosensor. una vez desarrollado el dispositivo, el electrodo de trabajo es modificado con la sonda de adn de cadena sencilla con el fin de formar una superficie de reconocimiento para la detecci\u00f3n de una secuencia espec\u00edfica de adn. La inmovilizaci\u00f3n de la sonda de captura de adn es llevada acabo mediante autoensamblaje de monocapas (self-assembled monolayers;sam), y es hibridada con su secuencia complementaria. La formaci\u00f3n de la superficie de reconocimiento y la detecci\u00f3n de la hibridaci\u00f3n son caracterizadas mediante dos t\u00e9cnicas electroqu\u00edmicas diferentes: espectroscop\u00eda de impedancia electroqu\u00edmica (electrochemical impedance spectroscopy;eis) y voltametr\u00eda c\u00edclica (cyclic voltammetry;cv). El par redox ferro\/ferricianuro pot\u00e1sico es utilizado como indicador electroqu\u00edmico.  la selectividad y la sensibilidad del biosensor son tambi\u00e9n estudiados exponiendo la superficie de reconocimiento a una cadena de adn no complementaria y a diferentes concentraciones de adn complementario. adem\u00e1s, con el fin de aumentar la selectividad del biosensor se ha utilizado un complejo de pentaamin rutenio (rul) ([ru-(nh3)5l]2+, donde l es [3-(2-fenantren-9-ilvinil-piridina]) como indicador electroqu\u00edmico para detectar la hibridaci\u00f3n. las diferencias obtenidas en la resistencia de transferencia de carga (rct), en el modulo de la impedancia ([z]) y en las corrientes de pico an\u00f3dico\/cat\u00f3dico (ipa, ipc) del ferro\/ferricianuro pot\u00e1sico y del complejo rul a partir de la hibridaci\u00f3n, demuestran que el biosensor desarrollado en este trabajo se podr\u00eda presentar como una prometedora alternativa para la detecci\u00f3n simple, de bajo coste y selectiva de secuencias espec\u00edficas de adn en un futuro cercano.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Electrochemical dna microbiosensor based on a three-electrode configuration for the detection of specific dna sequences<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Electrochemical dna microbiosensor based on a three-electrode configuration for the detection of specific dna sequences <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Larraitz A\u00f1orga G\u00f3mez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/10\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Sergio Arana Alonso<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: eduardo Ayesa iturralde <\/li>\n<li>Fernando Jos\u00e9 Arizti urquijo (vocal)<\/li>\n<li>tania Garc\u00eda mendiola (vocal)<\/li>\n<li>c\u00e1ndid Reig escriv\u00e1 (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Larraitz A\u00f1orga G\u00f3mez En este documento se presenta un microbiosensor electroqu\u00edmico para la detecci\u00f3n de secuencias espec\u00edficas [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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