{"id":104541,"date":"2018-03-11T10:27:35","date_gmt":"2018-03-11T10:27:35","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/reactivacion-de-agregados-proteicos-por-clpb-funcion-del-dominio-m\/"},"modified":"2018-03-11T10:27:35","modified_gmt":"2018-03-11T10:27:35","slug":"reactivacion-de-agregados-proteicos-por-clpb-funcion-del-dominio-m","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/proteinas\/reactivacion-de-agregados-proteicos-por-clpb-funcion-del-dominio-m\/","title":{"rendered":"Reactivaci\u00f3n de agregados proteicos por clpb. funci\u00f3n del dominio m"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Urko Del Castillo Rojo <\/strong><\/h2>\n<p>La agregaci\u00f3n proteica que ocurre en condiciones de estr\u00e9s ambiental compromete la viabilidad celular. Las c\u00e9lulas procariotas han desarrollado un sistema de chaperonas moleculares formado por clpb (hsp100) y el sistema dnak (dnak, dnaj y grpe) capaz de solubilizar y replegar estos agregados proteicos de una manera eficaz. En esta tesis se cuantifica termodin\u00e1micamente el equilibrio de asociaci\u00f3n de clpb en condiciones experimentales que mimetizan las condiciones fisiol\u00f3gicas y de estr\u00e9s. Este trabajo describe que clpb se encuentra en disoluci\u00f3n formando mon\u00f3meros, d\u00edmeros (tr\u00edmeros), hex\u00e1meros y dodec\u00e1meros. Este equilibrio de asociaci\u00f3n es fuertemente desplazado a la especie hexam\u00e9rica por la presencia de nucle\u00f3tidos, sin embargo se detecta que el atp estabiliza en mayor medida el hex\u00e1mero de clpb que el adp. Estas diferencias observadas indican que el intercambio de nucle\u00f3tido podr\u00eda modular la reorganizaci\u00f3n del hex\u00e1mero de clpb en la superficie del agregado proteico. Este trabajo demuestra que la actividad chaperona \u00f3ptima de clpb se alcanza cuando en el equilibrio de disociaci\u00f3n coexisten los hex\u00e1meros conformacionalmente heterog\u00e9neos con otros intermediarios de olig\u00f3merizaci\u00f3n m\u00e1s peque\u00f1os, fundamentalmente con mon\u00f3meros. tambi\u00e9n se observa que el dominio m de clpb, cuya localizaci\u00f3n espacial se desconoce con detalle, es un elemento estructural que estabiliza el olig\u00f3mero y que regula la actividad atpasa de los dos nbds en presencia de sustratos. finalmente, la formaci\u00f3n de hex\u00e1meros h\u00edbridos compuestos por diferentes combinaciones de subunidades silvestres y mutantes inactivas ha permitido observar que 4 subunidades del hex\u00e1mero de clpb forman la unidad funcional de la prote\u00edna necesaria para interaccionar y procesar los sustratos proteicos, siempre que uno de los anillos tengan todos los nbds inactivos.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Reactivaci\u00f3n de agregados proteicos por clpb. funci\u00f3n del dominio m<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Reactivaci\u00f3n de agregados proteicos por clpb. funci\u00f3n del dominio m <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Urko Del Castillo Rojo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 28\/10\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Arturo Muga Villate<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Luis Rodriguez arrondo <\/li>\n<li>german Rivas caballero (vocal)<\/li>\n<li>Jos\u00e9 Mar\u00eda Valpuesta moralejo (vocal)<\/li>\n<li>salvador Ventura zamora (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Urko Del Castillo Rojo La agregaci\u00f3n proteica que ocurre en condiciones de estr\u00e9s ambiental compromete la viabilidad [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[12909,3178],"tags":[70980,51885,134063,49522,96534,211245],"class_list":["post-104541","post","type-post","status-publish","format-standard","hentry","category-pais-vasco-euskal-herriko-unibertsitatea","category-proteinas","tag-arturo-muga-villate","tag-german-rivas-caballero","tag-jose-luis-rodriguez-arrondo","tag-jose-maria-valpuesta-moralejo","tag-salvador-ventura-zamora","tag-urko-del-castillo-rojo"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/104541","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=104541"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/104541\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=104541"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=104541"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=104541"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}