{"id":104741,"date":"2010-08-11T00:00:00","date_gmt":"2010-08-11T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/automation-of-early-lung-cancer-detection\/"},"modified":"2010-08-11T00:00:00","modified_gmt":"2010-08-11T00:00:00","slug":"automation-of-early-lung-cancer-detection","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/oncologia\/automation-of-early-lung-cancer-detection\/","title":{"rendered":"Automation of early lung cancer detection"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Thomas Pengo <\/strong><\/h2>\n<p>El c\u00e1ncer de pulm\u00f3n es entre los c\u00e1nceres con mortalidad m\u00e1s alta, el primero en tasa de mortalidad, con una tasa de supervivencia del 15% en los primeros 5 a\u00f1os despu\u00e9s del primer diagn\u00f3stico. El mayor obst\u00e1culo para bajar estas tasas de mortalidad tan altas es que el c\u00e1ncer frecuentemente se diagn\u00f3stica tarde, cuando ya se encuentra en un estadio avanzado y para las que existen pocas terapias eficaces. Por esta raz\u00f3n, hay un gran inter\u00e9s en la comunidad cient\u00edfica en adelantar el momento del diagn\u00f3stico a los estadios tempranos del c\u00e1ncer. El lavado broncho-alveolar es una t\u00e9cnica diagn\u00f3stica novedosa en la que un liquido es inyectado con alta presi\u00f3n en los pulmones del paciente utilizando una fibra \u00f3ptica y sucesivamente extra\u00eddo y analizado. Para analizar muestras de lba, utilizamos fiction, una t\u00e9cnica que combina un marcador de immunofluorescencia con hibridaci\u00f3n fluorescente in-situ (fish). El an\u00e1lisis de estas muestras necesita mucho tiempo y est\u00e1 abierta a errores, ya que el pat\u00f3logo necesita pasar muchas horas al microscopio de fluorescencia en condiciones dif\u00edciles. La idea del proyecto asociado con esta tesis es de automatizar este proceso. Para ello, se ha desarrollado una plataforma automatizada para microscop\u00eda \u00f3ptica, integrando un microscopio de epi-fluorescencia completamente automatizado con c\u00f3digo original que controla cada estadio de la adquisici\u00f3n y del an\u00e1lisis. La plataforma se ha combinado con un protocolo de an\u00e1lisis 2d\/3d para aislar c\u00e9lulas candidatas y, despu\u00e9s de reconstruir su estructura tridimensional, sugerir un diagn\u00f3stico para cada c\u00e9lula. Todos los resultados en cada paso del an\u00e1lisis pueden ser verificados para controlar su consistencia y validez. La plataforma y el an\u00e1lisis se han validado con varias muestras de lba en las que se han a\u00f1adido c\u00e9lulas de dos l\u00edneas celulares establecidas de cancer de pulm\u00f3n. Se cree que la plataforma tiene el potencial para ser aplicada a otras aplicaciones biom\u00e9dicas.   lung cancer is among the deadliest cancer types, ranking first in mortality rates, with a 15% survival rate over the first five years after diagnosis. The main hurdle in lowering these high mortality rates is that the cancer is often detected too late, when it is already at an advanced stage and only few effective cures exist. There is therefore a great interest in the research community to bring the moment of detection back to the early stages of the cancer. Broncho-alveolar lavage is a novel diagnostic technique in which a high pressure liquid is injected in the lungs of the patient using an optical fibre and then successively extracted and analyzed. To analyze bal samples, we use fiction, a technique which combines an immunomarker with fluorescence in-situ hybridization (fish). The analysis of these samples is time consuming and error-prone, as many hours in difficult conditions have to be spent by the pathologist at the fluorescent microscope. The idea of the project associated with the present dissertation is to automate this process. For this purpose, an automated platform for light microscopy has been developed by integrating a fully automated epi-fluorescent microscope with in-house developed software controlling every stage of the acquisition and the analysis. The platform has been combined with a 2d\/3d analysis protocol to isolate candidate cells and, after reconstructing their tridimensional structure, suggest a diagnosis on a per cell basis. All results at each step of the analysis can be verified for consistency and correctness. The platform and the analysis have been validated on various bal samples sprinkled with cells from two established cancer cell lines. The platform is believed to have the potencial to be applied to other biomedical applications.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Automation of early lung cancer detection<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Automation of early lung cancer detection <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Thomas Pengo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 08\/11\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Carlos Ortiz De Solorzano Aurusa<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Luis Montuenga badia <\/li>\n<li>alejandro Frangi  caregnato (vocal)<\/li>\n<li>michal Kozubek (vocal)<\/li>\n<li>norberto Malpica gonzalez de la vega (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Thomas Pengo El c\u00e1ncer de pulm\u00f3n es entre los c\u00e1nceres con mortalidad m\u00e1s alta, el primero en [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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