{"id":104796,"date":"2010-12-11T00:00:00","date_gmt":"2010-12-11T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-la-respuesta-de-las-celulas-estelares-hepaticas-a-la-carencia-de-aminoacidos\/"},"modified":"2010-12-11T00:00:00","modified_gmt":"2010-12-11T00:00:00","slug":"caracterizacion-de-la-respuesta-de-las-celulas-estelares-hepaticas-a-la-carencia-de-aminoacidos","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica\/caracterizacion-de-la-respuesta-de-las-celulas-estelares-hepaticas-a-la-carencia-de-aminoacidos\/","title":{"rendered":"Caracterizaci\u00f3n de la respuesta de las c\u00e9lulas estelares hep\u00e1ticas a la carencia de amino\u00e1cidos"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elena Arriazu Ruiz <\/strong><\/h2>\n<p>During amino acid deprivation, gcn2 kinase becomes phosphorylated and inhibits translation factor eif2&#945;; thus, lowering protein synthesis to allow cell survival under stress conditions. Aim: we hypothesized that amino acid deprivation could modulate the generation of reactive oxygen species (ros) in hepatic stellate cells (hsc), and as a result, regulate collagen i synthesis and the fibrogenic reponse.  results: histidine deprivation decreased h2o2 levels in hsc and promoted cell survival. The histidine-dependent decrease in h2o2 levels correlated with phosphorylation of gcn2. Incubation with salubrinal, an inhibitor of the eif2&#945; phosphatase, demonstrated that eif2&#945; phosphorylation and h2o2 depletion are independent events, thus suggesting that gcn2 does not function as an eif2&#945; kinase in this context. Incubation with histidine-free medium caused an increase in superoxide radical levels and down-regulated sod activity in hsc. Histidine deprivation also decreased intra- and extracellular protein levels collagen i and col1a1 and col1a2 mrna levels. Studies performed in animal models for liver injury using knock out mice for gcn2, showed higher necroinflammatory scores in gcn2-\/- mice treated with ccl4 than in wt mice; greater focal necrosis and portal inflammation as well as enhanced levels of transaminases alt and ast were also observed. Moreover, gcn2-\/- mice showed a higher deposition of collagen type i even in the absence of ccl4 treatment. Conclusion: these results suggest that gcn2 could play a role in down-regulating h2o2 levels and collagen i synthesis in hsc, which in turn may decrease the fibrogenic response of these cells to liver injury.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n de la respuesta de las c\u00e9lulas estelares hep\u00e1ticas a la carencia de amino\u00e1cidos<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n de la respuesta de las c\u00e9lulas estelares hep\u00e1ticas a la carencia de amino\u00e1cidos <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elena Arriazu Ruiz <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 12\/11\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda Jose Iraburu Elizalde<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan Sastre belloch <\/li>\n<li>Elena Ruiz Garc\u00eda-trevijano (vocal)<\/li>\n<li>natalia Nieto (vocal)<\/li>\n<li>mat\u00edas Antonio \u00e1vila zaragoz\u00e1 (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elena Arriazu Ruiz During amino acid deprivation, gcn2 kinase becomes phosphorylated and inhibits translation factor eif2&#945;; thus, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[160,17749],"tags":[211652,156298,34448,41611,42715,211653],"class_list":["post-104796","post","type-post","status-publish","format-standard","hentry","category-bioquimica","category-navarra","tag-elena-arriazu-ruiz","tag-elena-ruiz-garcia-trevijano","tag-juan-sastre-belloch","tag-maria-jose-iraburu-elizalde","tag-matias-antonio-avila-zaragoza","tag-natalia-nieto"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/104796","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=104796"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/104796\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=104796"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=104796"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=104796"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}