{"id":106113,"date":"2018-03-11T10:29:54","date_gmt":"2018-03-11T10:29:54","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/new-insights-into-the-molecular-mechanisms-of-rhodopsin-retinitis-pigmentosa\/"},"modified":"2018-03-11T10:29:54","modified_gmt":"2018-03-11T10:29:54","slug":"new-insights-into-the-molecular-mechanisms-of-rhodopsin-retinitis-pigmentosa","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/new-insights-into-the-molecular-mechanisms-of-rhodopsin-retinitis-pigmentosa\/","title":{"rendered":"New insights into the molecular mechanisms of rhodopsin retinitis pigmentosa"},"content":{"rendered":"<h2>Tesis doctoral de <strong> M\u00f3nica Aguil\u00c1\u00a0 Cerd\u00c1\u00a0 <\/strong><\/h2>\n<p>Rhodopsin is the visual photoreceptor responsible for dim light vision. Mutations in rhodopsin  are the most common cause of the severe inherited neurodegenerative blindness, retinitis  pigmentosa (rp).    one of the goals of this work was to establish the relationship between the structure and the  function of rp mutants, mainly using spectroscopic techniques. Briefly, the study was focused  on rhodopsin mutations that affect the ligand bindind pocket. The principal interest was to  determine the illumination response and the ability to activate transducin with the rhodopsin  mutants. The results showed retinal regeneration problems as well as low activation rates. The  next step was to use the retinal analog 11-cis-7 methylretinal to try to unravel the conformational  changes that take place when rhodopsin moves to its active form. The results obtained with the  combined use of a 7-methyl retinal analog and rhodopsin mutants highlight the tight coupling  between the chromophore and the receptor for optimal structure and stability of dark and active  conformations of the rod photosensitive pigment. The results with the m207 mutants confirm the  specific role of this amino acid in the correctly folded conformation of the receptor and in the  activation process.    another main outline of the project was the study of the cellular trafficking of rp rhodopsin  mutants g114d, m207r and r135l. An extensive cellular characterization in cell culture models  was carried out. Several pharmacological compounds were tested in this model in an attempt to  improve their folding or trafficking defects. Manipulation of molecular chaperones, treatment with  chemical chaperones or heat shock protein inducers were used to reduce inclusion incidence,  alleviate cell death and promoted the translocation of the mutant protein to the plasma  membrane.    to explore different ways of analyzing effects of rhodopsin mutations on its function, we set up  the use of surface plasmon resonance as a new tool for studying activation of recombinant  rhodopsin. Our success in the immobilization of recombinant rhodopsin allows not only the study  of transducin activation but also the possibility to determine the kinetic data of rhodopsin- transducin interaction. The use of this tool can be applied for future studies of recombinant  rhodopsin mutants.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>New insights into the molecular mechanisms of rhodopsin retinitis pigmentosa<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 New insights into the molecular mechanisms of rhodopsin retinitis pigmentosa <\/li>\n<li><strong>Autor:<\/strong>\u00a0 M\u00f3nica Aguil\u00c1\u00a0 Cerd\u00c1\u00a0 <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de catalunya<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 21\/12\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Pere Garriga Sol\u00e9<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  Jes\u00fas Perez gonzalez <\/li>\n<li>michael Cheetham (vocal)<\/li>\n<li>esteve Padr\u00f3s morell (vocal)<\/li>\n<li>joan Manyosa ribatallada (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de M\u00f3nica Aguil\u00c1\u00a0 Cerd\u00c1\u00a0 Rhodopsin is the visual photoreceptor responsible for dim light vision. Mutations in rhodopsin are [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[849,36483,15596],"tags":[2843,21274,15620,213712,213711,96552],"class_list":["post-106113","post","type-post","status-publish","format-standard","hentry","category-biologia-celular","category-biologia-molecular","category-politecnica-de-catalunya","tag-esteve-padros-morell","tag-joan-manyosa-ribatallada","tag-juan-jesus-perez-gonzalez","tag-michael-cheetham","tag-manica-aguila-cerda","tag-pere-garriga-sole"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/106113","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=106113"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/106113\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=106113"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=106113"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=106113"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}