{"id":106171,"date":"2018-03-11T10:29:59","date_gmt":"2018-03-11T10:29:59","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/agentes-nosocomiais-emergentes-analise-citometrica-de-cryptosporidium-encephalitozoon-pneumocystis-e-giardia\/"},"modified":"2018-03-11T10:29:59","modified_gmt":"2018-03-11T10:29:59","slug":"agentes-nosocomiais-emergentes-analise-citometrica-de-cryptosporidium-encephalitozoon-pneumocystis-e-giardia","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/parasitologia-humana\/agentes-nosocomiais-emergentes-analise-citometrica-de-cryptosporidium-encephalitozoon-pneumocystis-e-giardia\/","title":{"rendered":"Agentes nosocomiais emergentes: an\u00e1lise citom\u00e9trica de cryptosporidium, encephalitozoon, pneumocystis e giardia"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Joana Margarida Moutinho  Barbosa <\/strong><\/h2>\n<p>Las parasitosis son de las enfermedades m\u00e1s prevalentes en el mundo, sobre todo en los pa\u00edses en desarrollo y en los desarrollados debido al aumento de poblaci\u00f3n inmunocoprometida. El objetivo principal de este trabajo ha sido desarrollar un protocolo para la detecci\u00f3n de cryptosporidium parvum, giardia lamblia, encephalitozoon intestitnalis y pneumocytis jirovecii en muestras cl\u00ednicas e ambientales usando citometr\u00eda de flujo (cf) y se estudi\u00f3 la eficacia de agentes f\u00edsico-qu\u00edmicos sobre g. Lamblia. Se utilizaron suspensiones puras de protozoos para la optimizaci\u00f3n del protocolo de detecci\u00f3n citom\u00e9trica y para p. Jirovecii, se usaron muestras respiratorias positivas y negativas, previamente caracterizadas por microscop\u00eda de fluorescencia. Se estudi\u00f3 el marcaje con yoduro de propidio (ip) para evaluar la viabilidad de cada microorganismo  y se realizaron estudios  del l\u00edmite de detecci\u00f3n citom\u00e9trico y de especificidad del marcaje.  Se optimizaron diferentes par\u00e1metros del m\u00e9todo de faust para las muestras fecales. Suspensiones de cistos de f. Lamblia fueron usados para la evaluaci\u00f3n de la eficacia de agentes qu\u00edmicos como etanol (70% y 100%) e hipoclorito s\u00f3dico (1 mg\/l) y f\u00edsicos como calor (65\u00c2\u00bac) y radiaci\u00f3n uv, utilizando marcadores de \u00e1cidos nucleicos dapi (marcador  de viabilidad) e ip (marcador de muerte celular).  La optimizaci\u00f3n  del protocolo de an\u00e1lisis y concentraciones de los respectivos fluorocromos fueron determinados jpara cada agente. Para todos los agentes f\u00edsico-qu\u00edmicos estudiados fue posible determinar correlaciones estad\u00edsitcamente  significativas entre el m\u00e9todo de cultivo y la cf. Con la elaboraci\u00f3n y optimizaci\u00f3n de los protocolos citom\u00e9tricos mostrados en este trabajo ahora ser\u00eda posible su aplicaci\u00f3n en la ruina del laboratorio para la detecci\u00f3n de estos agentes oportunistas tanto en muestras cl\u00ednicas y ambientales como en agua y en alimentos. se puede concluir que la citometr\u00eda de flujo representa un nuevo m\u00e9todo de diagn\u00f3stico con una elevada sensibilidad y especificidad, r\u00e1pido y alternativo a las t\u00e9cnicas cl\u00e1sicas in vitro, con numerosas apliaciones en cl\u00ednica.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Agentes nosocomiais emergentes: an\u00e1lise citom\u00e9trica de cryptosporidium, encephalitozoon, pneumocystis e giardia<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Agentes nosocomiais emergentes: an\u00e1lise citom\u00e9trica de cryptosporidium, encephalitozoon, pneumocystis e giardia <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Joana Margarida Moutinho  Barbosa <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Vigo<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 21\/12\/2010<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda Jos\u00e9 P\u00e9rez \u00e1lvarez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: raul Iglesias blanco <\/li>\n<li>isabel alexandra Rodrigues longo ribeiro (vocal)<\/li>\n<li>carmen Mar\u00eda Lisboa  silva (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Joana Margarida Moutinho Barbosa Las parasitosis son de las enfermedades m\u00e1s prevalentes en el mundo, sobre todo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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