{"id":106645,"date":"2011-04-02T00:00:00","date_gmt":"2011-04-02T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/stepwise-differentiation-of-pancreatic-acinar-cells-from-mes-cells-by-manipulating-signalling-pathway\/"},"modified":"2011-04-02T00:00:00","modified_gmt":"2011-04-02T00:00:00","slug":"stepwise-differentiation-of-pancreatic-acinar-cells-from-mes-cells-by-manipulating-signalling-pathway","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-humana\/stepwise-differentiation-of-pancreatic-acinar-cells-from-mes-cells-by-manipulating-signalling-pathway\/","title":{"rendered":"Stepwise differentiation of pancreatic acinar cells from mes cells by manipulating signalling pathway"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Fabien Delaspre <\/strong><\/h2>\n<p>Aunque se conoce la implicaci\u00f3n de las c\u00e9lulas pancre\u00e1ticas acinares en patolog\u00edas exocrinas (pancreatitis y c\u00e1ncer de p\u00e1ncreas), la falta de modelos normales basados en c\u00e9lulas ha limitado el estudio de las alteraciones que suceden en el programa de diferenciaci\u00f3n pancre\u00e1tica. Hemos demostrado previamente que las c\u00e9lulas madre embrionarias murinas, que son pluripotentes, pueden adquirir un fenotipo acinar in vitro. Esto se consigui\u00f3, en parte, con una combinaci\u00f3n de se\u00f1ales que proced\u00edan del cultivo de p\u00e1ncreas fetales que, pero, no era espec\u00edfico del linaje pancre\u00e1tico. El objetivo de este trabajo fue el de desarrollar un protocolo selectivo para el linaje acinar basado en la activaci\u00f3n secuencial de v\u00edas de se\u00f1alizaci\u00f3n que recapitulen el desarrollo pancre\u00e1tico in vivo, a trav\u00e9s de la formaci\u00f3n definitiva del endodermo, la especificaci\u00f3n pancre\u00e1tica y acinar y la expansi\u00f3n\/diferenciaci\u00f3n de progenitores acinares. El tratamiento de cuerpos embrionarios con activina a promovi\u00f3 la expresi\u00f3n de genes de endodermo como est\u00e1 previamente descrito. El tratamiento subsecuente con \u00e1cido retinoico, fgf10 y ciclopamina, un inhibidor de la v\u00eda de hedgehog, result\u00f3 en la inducci\u00f3n de los marcadores de progenitores pancre\u00e1ticos pdx1, ptf1a y cpa1 pero tambi\u00e9n de aquellos expresados en el linaje pancre\u00e1tico, que fueron reducidos con la inhibici\u00f3n de bmps. Las c\u00e9lulas fueron a continuaci\u00f3n cultivadas en matrigel utilizando un sistema de cultivo en 3d en presencia de folistatina, dexametasona y kgf comportando una inducci\u00f3n significativa de los niveles de mrna y prote\u00edna de marcadores acinares y una disminuci\u00f3n de la expresi\u00f3n de los de marcadores acinares. Adem\u00e1s, se vio que amyl se secretaba en el medio. Estos datos indican que la activaci\u00f3n selectiva del programa de diferenciaci\u00f3n acinar en c\u00e9lulas madre embrionarias se puede llevar a cabo mediante una inducci\u00f3n escalonada de v\u00edas de se\u00f1alizaci\u00f3n involucradas en el desarrollo pancre\u00e1tico exocrino proporcionando una herramienta potencial para estudiar la diferenciaci\u00f3n pancre\u00e1tica y enfermedades relacionadas con el p\u00e1ncreas.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Stepwise differentiation of pancreatic acinar cells from mes cells by manipulating signalling pathway<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Stepwise differentiation of pancreatic acinar cells from mes cells by manipulating signalling pathway <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Fabien Delaspre <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pompeu fabra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 04\/02\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Anouchka Skoudy Haym<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: rosa Mar\u00eda Gasa arnaldich <\/li>\n<li>pierre-yves Bourillot (vocal)<\/li>\n<li>  (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Fabien Delaspre Aunque se conoce la implicaci\u00f3n de las c\u00e9lulas pancre\u00e1ticas acinares en patolog\u00edas exocrinas (pancreatitis y [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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