{"id":107145,"date":"2011-03-03T00:00:00","date_gmt":"2011-03-03T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/engineering-synthetic-spatial-patterning-systems-in-mammalian-cells\/"},"modified":"2011-03-03T00:00:00","modified_gmt":"2011-03-03T00:00:00","slug":"engineering-synthetic-spatial-patterning-systems-in-mammalian-cells","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/engineering-synthetic-spatial-patterning-systems-in-mammalian-cells\/","title":{"rendered":"Engineering synthetic spatial patterning systems in mammalian cells"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Andreia Carvalho <\/strong><\/h2>\n<p>La biolog\u00eda sint\u00e9tica es una disciplina floreciente que representa una gran promesa, al tratar de construir sistemas biol\u00f3gicos que pueden probar los l\u00edmites de nuestra comprensi\u00f3n. En la \u00faltima d\u00e9cada, el campo ha avanzado mucho con varios circuitos de genes sint\u00e9ticos que han sido  desarrollados, incluyendo los interruptores gen\u00e9ticos, los osciladores, los m\u00f3dulos de comunicaci\u00f3n intercelular y redes de comunicaci\u00f3n multicelulares, aunque la gran mayor\u00eda fueron construidos en las bacterias y levaduras. En esta tesis se establece el dise\u00f1o, construcci\u00f3n y caracterizaci\u00f3n de un sistema artificial sint\u00e9tico de comunicaci\u00f3n intercelular en c\u00e9lulas de mam\u00edfero. Este sistema fue utilizado para dise\u00f1ar patrones espaciales: gradientes de la activaci\u00f3n o de represi\u00f3n de un gen reportero fluorescente en un campo de c\u00e9lulas. El sistema modelo utilizado fue la l\u00ednea celular de c\u00e9lulas de ri\u00f1\u00f3n canino madin-darby (mdck), que forma una monocapa polarizada que encierra un lumen lleno de l\u00edquido (quistes) cuando crecen en una matriz de col\u00e1geno. Estos quistes fueron utilizados como un para la ingenier\u00eda de redes de genes sint\u00e9ticos y formaci\u00f3n de patrones, en un contexto tridimensional. Para esto inicialmente desarroll\u00e9 un m\u00e9todo para transfectar quistes mdck a nivel local, logrando distintas regiones de transfecci\u00f3n. Entonces constru\u00ed y caracteric\u00e9 un gen reportero fluorescente que es inducido por el factor de crecimiento de hepatocitos y reprimido por una variante truncada, nk4. Por \u00faltimo, dise\u00f1\u00e9 una plataforma de s\u00edntesis espacial del patr\u00f3n para la microscop\u00eda de campo ancho, basado en la expresi\u00f3n y secreci\u00f3n localizada de hgf o nk4. El primero difunde a partir de c\u00e9lulas origen y modula la formaci\u00f3n de t\u00fabulos y la expresi\u00f3n del gen reportero fluorescente en las c\u00e9lulas del receptor, mientras que nk4 reprime este efecto.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Engineering synthetic spatial patterning systems in mammalian cells<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Engineering synthetic spatial patterning systems in mammalian cells <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Andreia Carvalho <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pompeu fabra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 03\/03\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mark Isalan<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Antonio Garcia de herreros madue\u00f1o <\/li>\n<li>ricard Vicente sole (vocal)<\/li>\n<li>  (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Andreia Carvalho La biolog\u00eda sint\u00e9tica es una disciplina floreciente que representa una gran promesa, al tratar de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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