{"id":108050,"date":"2018-03-11T10:32:43","date_gmt":"2018-03-11T10:32:43","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/nicotiana-tabacum-l-cv-xanthi-como-sistema-heterologo-para-la-produccion-de-lactogeno-placentario-humano\/"},"modified":"2018-03-11T10:32:43","modified_gmt":"2018-03-11T10:32:43","slug":"nicotiana-tabacum-l-cv-xanthi-como-sistema-heterologo-para-la-produccion-de-lactogeno-placentario-humano","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/pais-vasco-euskal-herriko-unibertsitatea\/nicotiana-tabacum-l-cv-xanthi-como-sistema-heterologo-para-la-produccion-de-lactogeno-placentario-humano\/","title":{"rendered":"Nicotiana tabacum l. cv xanthi como sistema heter\u00f3logo para la producci\u00f3n de lact\u00f3geno placentario humano"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Iratxe Urreta Gomez <\/strong><\/h2>\n<p>El lact\u00f3geno placentario humano (hpl) es una hormona polipept\u00eddica que forma parte de la peque\u00f1a lista de factores del crecimiento capaces de tratar la diabetes tipo 1 mediante el transplante de islotes humanos. Esta hormona tiene la capacidad de mejorar la supervivencia de los islotes humanos antes y\/o despu\u00e9s de su transplante. En este trabajo se aborda la producci\u00f3n de hpl en sistemas heter\u00f3logos vegetales basados en nicotiana tabacum cv xanthi. El cdna del hpl ha sido introducido en el genoma vegetal del tabaco mediante agrobacterium tumefaciens, portando los vectores de expresi\u00f3n pnekhpl1 y pnekhpl2. La actividad transcripcional se ha confirmado mediante pcr en tiempo real, y los niveles de producci\u00f3n de hpl en plantas transg\u00e9nicas han alcanzado el 1% de la cantidad de prote\u00edna total soluble (pts). Tras purificar la prote\u00edna mediante cromatograf\u00eda de afinidad a iones met\u00e1licos, se ha testado su actividad biol\u00f3gica. Los ensayos in vitro de proliferaci\u00f3n y se\u00f1alizaci\u00f3n intracelular han demostrado que la prote\u00edna producida en plantas mantiene su actividad intacta, y por lo tanto, que las plantas son capaces de producir la hormona funcional. En este trabajo tambi\u00e9n se han caracterizado la estabilidad de la prote\u00edna hpl a lo largo del ciclo de vida del tabaco, y su transmisi\u00f3n a la siguiente generaci\u00f3n. La cantidad de prote\u00edna recombinante en las hojas j\u00f3venes de las plantas cultivadas en el invernadero ha sufrido un 26% de disminuci\u00f3n tras 90 d\u00edas de cultivo, obteniendo el m\u00e1ximo de prote\u00edna a los 30 d\u00edas. La progenie de plantas pnekhpl2 han alcanzado el 1.1% de la pts. Tambi\u00e9n se ha analizado la producci\u00f3n de hpl en callos obtenidos a partir de tejido foliar de plantas transg\u00e9nicas, que se han utilizado para establecer suspensiones celulares. En este sistema se han obtenido 280.5 \u00c2\u00b5g l-1 de hpl total demostrando que tanto las plantas como las suspensiones son sistemas id\u00f3neos para producir hpl recombinante<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Nicotiana tabacum l. cv xanthi como sistema heter\u00f3logo para la producci\u00f3n de lact\u00f3geno placentario humano<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Nicotiana tabacum l. cv xanthi como sistema heter\u00f3logo para la producci\u00f3n de lact\u00f3geno placentario humano <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Iratxe Urreta Gomez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/04\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Sonia Casta\u00f1on  De La Torre<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: mercedes Renobales scheifler <\/li>\n<li>ricardo Javier Ordas fernandez (vocal)<\/li>\n<li>pedro Mar\u00eda Aparicio tejo (vocal)<\/li>\n<li>jon mirena Veramenti charola (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Iratxe Urreta Gomez El lact\u00f3geno placentario humano (hpl) es una hormona polipept\u00eddica que forma parte de la [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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