{"id":108391,"date":"2018-03-11T10:33:09","date_gmt":"2018-03-11T10:33:09","guid":{"rendered":""},"modified":"2018-03-11T10:33:09","modified_gmt":"2018-03-11T10:33:09","slug":"papel-de-una-etanolamina-fosfato-transferasa-y-una-glicosil-transferasa-en-la-sa%c2%adntesis-del-lipopolisacarido-de-brucella-y-utilizacion-de-una-acetil-transferasa-para-la-modificacion-epitopica-de","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/microbiologia\/papel-de-una-etanolamina-fosfato-transferasa-y-una-glicosil-transferasa-en-la-sa%c2%adntesis-del-lipopolisacarido-de-brucella-y-utilizacion-de-una-acetil-transferasa-para-la-modificacion-epitopica-de\/","title":{"rendered":"Papel de una etanolamina-fosfato transferasa y una glicosil transferasa en la s\u00edntesis del lipopolisac\u00e1rido de brucella y utilizaci\u00f3n de una acetil transferasa para la modificaci\u00f3n epit\u00f3pica de la cadena o"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Yolanda Teresa Gil Ram\u00edrez <\/strong><\/h2>\n<p>Brucellosis is a disease of terrestrial and marine mammals and an important zoonosis. Brucella lipopolysaccharide plays a major role in virulence and is considered as a molecule with a modified pathogen-associated molecular pattern. The role of two hypothetical lipopolysaccharide core glycosyltransferases [bab1_0351 (wadb) and bab1_1620] and a putative phosphoethanolamine transferase (bmei0118) was investigated.  a wadb mutant in b. Abortus, b. Melitensis and b. Suis biovar 2 lacked part of the lipopolysaccharide core but kept the o-chain. The wadb b. Abortus mutant was sensitive to normal serum and bactericidal peptides and attenuated in dendritic cells and mice. Moreover, it elicited a protective immunoresponse, confirming that core mutants are promising brucellosis vaccines. On the other hand, bab1_1620, seems not to be related to this process in b. Abortus and its function is not essential for full virulence in mice.  it is demonstrated that bmelpta acts as a transferase that incorporate phosphoethanolamine to the lipid a section. This modification contributes to low recognition of lipopolysaccharide by some innate immune effectors since bmelpta mutant was sensitive to normal serum and bactericidal peptides. Bmelpta has orthologous in other alpha-2 proteobacteria such as ochrobactrum, sinorhizobium or agrobacterium.  a crucial aspect in the control of brucellosis is that the use of s-brucellae vaccines interferes in the diagnosis, since they generate antibodies against the o-chain of the lipopolysaccharide, which is also the dominant antigen in infected animals. In order to bypass this difficulty, we have followed a new approach. The expression of wbdr (e. Coli o 157:h7 o chain acetyltransferase) in b. Abortus 2308 leads to the incorporation of acetyl residues to the perosamine polysaccharide, creating a new immunogenic epitope that could be the base for the elaboration of new diagnostic tests.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Papel de una etanolamina-fosfato transferasa y una glicosil transferasa en la s\u00edntesis del lipopolisac\u00e1rido de brucella y utilizaci\u00f3n de una acetil transferasa para la modificaci\u00f3n epit\u00f3pica de la cadena o<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Papel de una etanolamina-fosfato transferasa y una glicosil transferasa en la s\u00edntesis del lipopolisac\u00e1rido de brucella y utilizaci\u00f3n de una acetil transferasa para la modificaci\u00f3n epit\u00f3pica de la cadena o <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Yolanda Teresa Gil Ram\u00edrez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/05\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Ignacio Moriyon Uria<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ignacio Lopez go\u00f1i <\/li>\n<li>Jos\u00e9 Mar\u00eda Blasco Martinez (vocal)<\/li>\n<li>Jos\u00e9 Antonio Bengoechea alonso (vocal)<\/li>\n<li>michel Zygmunt (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Yolanda Teresa Gil Ram\u00edrez Brucellosis is a disease of terrestrial and marine mammals and an important zoonosis. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36606,278,17749,1946],"tags":[53779,10696,55647,43302,217287,217286],"class_list":["post-108391","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular-de-microorganismos","category-microbiologia","category-navarra","category-produccion-animal","tag-ignacio-lopez-goni","tag-ignacio-moriyon-uria","tag-jose-antonio-bengoechea-alonso","tag-jose-maria-blasco-Martinez","tag-michel-zygmunt","tag-yolanda-teresa-gil-ramirez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/108391","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=108391"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/108391\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=108391"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=108391"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=108391"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}