{"id":110314,"date":"2018-03-11T10:36:14","date_gmt":"2018-03-11T10:36:14","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/sustitucion-del-gen-nira-entre-cianobacterias-termofa%c2%adlicas-clonacion-expresion-y-purificacion-de-la-nitrito-reductasa-de-thermosynechococcus-elongatus-bp-1\/"},"modified":"2018-03-11T10:36:14","modified_gmt":"2018-03-11T10:36:14","slug":"sustitucion-del-gen-nira-entre-cianobacterias-termofa%c2%adlicas-clonacion-expresion-y-purificacion-de-la-nitrito-reductasa-de-thermosynechococcus-elongatus-bp-1","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/sustitucion-del-gen-nira-entre-cianobacterias-termofa%c2%adlicas-clonacion-expresion-y-purificacion-de-la-nitrito-reductasa-de-thermosynechococcus-elongatus-bp-1\/","title":{"rendered":"Sustituci\u00f3n del gen nira entre cianobacterias termof\u00edlicas. clonaci\u00f3n, expresi\u00f3n y purificaci\u00f3n de la nitrito reductasa de thermosynechococcus elongatus bp-1"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Maite Buxens Azcoaga <\/strong><\/h2>\n<p>Las cianobacterias son organismos procariotas, gram-negativos que se caracterizan por llevar a cabo la fotos\u00edntesis oxig\u00e9nica. Constituyen un grupo muy heterog\u00e9neo pero en todas ellas el nitr\u00f3geno representa un alto porcentaje de su peso seco. El nitr\u00f3geno puede ser asimilado de distintas formas. En el caso de phormidium laminosum y thermosynechococcus elongatus bp-1, el nitr\u00f3geno  entra en las c\u00e9lulas como amonio, nitrato y nitrito. P. Laminosum y t. Elongatus bp-1 son dos cianobacterias que poseen una organizaci\u00f3n similar de los genes responsables de la toma y asimilaci\u00f3n de nitrato\/nitrito.  en este trabajo se ha sustituido el gen que codifica la nitrito reductasa de t. Elongatus bp-1 (primer gen de este oper\u00f3n) por el correspondiente de p. Laminosum. Para realizar el proceso se ha empleado la t\u00e9cnica long flanking homology-pcr (lfh-pcr) y la capacidad que muestra t. Elongatus bp-1 para adquirir dna ex\u00f3geno de forma natural. De esta manera, a lo largo del desarrollo, se han obtenido dos tipos celulares distintos. En primer lugar, se han construido c\u00e9lulas de t. Elongatus bp-1 carentes del gen nira, y posteriormente, c\u00e9lulas de t. Elongatus bp-1 complementadas con nira de p. Laminosum    adem\u00e1s, con el objetivo de estudiar el metabolismo del nitr\u00f3geno de t. Elongatus bp1, se ha clonado, expresado y purificado la prote\u00edna nira, que codifica la actividad nitrito reductasa, comprob\u00e1ndose que en esta prote\u00edna existe una zona especialmente susceptible a la  hidr\u00f3lisis o accesible a las proteasas.  finalmente se han obtenido anticuerpos policlonales anti-nira, que han permitido hacer un seguimiento detallado in vivo de la expresi\u00f3n de la prote\u00edna nira en c\u00e9lulas silvestres de t. Elongatus bp-1.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Sustituci\u00f3n del gen nira entre cianobacterias termof\u00edlicas. clonaci\u00f3n, expresi\u00f3n y purificaci\u00f3n de la nitrito reductasa de thermosynechococcus elongatus bp-1<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Sustituci\u00f3n del gen nira entre cianobacterias termof\u00edlicas. clonaci\u00f3n, expresi\u00f3n y purificaci\u00f3n de la nitrito reductasa de thermosynechococcus elongatus bp-1 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Maite Buxens Azcoaga <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/07\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>M\u00aa Jes\u00fas Llama Fontal<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Mar\u00eda Vega piqueres <\/li>\n<li>Jos\u00e9 Berenguer Carlos (vocal)<\/li>\n<li>carmen Gonzalez murua (vocal)<\/li>\n<li>Fernando De la cruz calahorra (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Maite Buxens Azcoaga Las cianobacterias son organismos procariotas, gram-negativos que se caracterizan por llevar a cabo la [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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