{"id":110333,"date":"2018-03-11T10:36:19","date_gmt":"2018-03-11T10:36:19","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/dissecting-the-roles-of-nup35-and-nup107-in-nuclear-pore-complex-assembly-and-mitosis-in-caenorhabditis-elegans\/"},"modified":"2018-03-11T10:36:19","modified_gmt":"2018-03-11T10:36:19","slug":"dissecting-the-roles-of-nup35-and-nup107-in-nuclear-pore-complex-assembly-and-mitosis-in-caenorhabditis-elegans","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/pablo-de-olavide\/dissecting-the-roles-of-nup35-and-nup107-in-nuclear-pore-complex-assembly-and-mitosis-in-caenorhabditis-elegans\/","title":{"rendered":"Dissecting the roles of nup35 and nup107 in nuclear pore complex assembly and mitosis in caenorhabditis elegans"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Eduardo Rodenas Martinez <\/strong><\/h2>\n<p>Nuclear pore complexes consist of several subcomplexes. The nup107 complex is important for nucleocytoplasmic transport, nuclear envelope assembly, and kinetochore function. However, the underlying molecular mechanisms and the roles of individual complex members remain elusive. We report the first description of a genetic disruption of nup107 in a metazoan. Caenorhabditis elegans nup107\/npp-5 mutants display temperature-dependent lethality. Surprisingly, npp-5 is dispensable for incorporation of most nucleoporins into nuclear pores and for nuclear protein import. In contrast, npp-5 is essential for proper kinetochore localization of nup133\/npp-15, another nup107 complex member, whereas recruitment of nup96\/npp-10c and elys\/mel-28 is npp-5 independent. We found that kinetochore protein nuf2\/him-10 and aurora b\/air-2 kinase are less abundant on mitotic chromatin upon npp-5 depletion. Npp-5 mutants are hypersensitive to anoxia, suggesting that the spindle assembly checkpoint (sac) is compromised. Indeed, npp-5 interacts genetically and physically with sac protein mad1\/mdf-1, whose nuclear envelope accumulation requires npp-5. Thus our results strengthen the emerging connection between nuclear pore proteins and chromosome segregation.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Dissecting the roles of nup35 and nup107 in nuclear pore complex assembly and mitosis in caenorhabditis elegans<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Dissecting the roles of nup35 and nup107 in nuclear pore complex assembly and mitosis in caenorhabditis elegans <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Eduardo Rodenas Martinez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pablo de olavide<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 18\/07\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Peter Askjaer<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: andr\u00e9s Aguilera l\u00f3pez <\/li>\n<li>Antonio Miranda vizuete (vocal)<\/li>\n<li>vincent Galy (vocal)<\/li>\n<li>tatiana Garc\u00eda muse (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Eduardo Rodenas Martinez Nuclear pore complexes consist of several subcomplexes. The nup107 complex is important for nucleocytoplasmic [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[37286],"tags":[10985,20891,220307,138789,67336,220308],"class_list":["post-110333","post","type-post","status-publish","format-standard","hentry","category-pablo-de-olavide","tag-andres-aguilera-lopez","tag-antonio-miranda-vizuete","tag-eduardo-rodenas-Martinez","tag-peter-askjaer","tag-tatiana-garcia-muse","tag-vincent-galy"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/110333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=110333"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/110333\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=110333"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=110333"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=110333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}