{"id":110516,"date":"2018-03-11T10:36:35","date_gmt":"2018-03-11T10:36:35","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/regulacion-por-nitrogeno-en-pseudomonas-putida\/"},"modified":"2018-03-11T10:36:35","modified_gmt":"2018-03-11T10:36:35","slug":"regulacion-por-nitrogeno-en-pseudomonas-putida","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica\/regulacion-por-nitrogeno-en-pseudomonas-putida\/","title":{"rendered":"Regulaci\u00f3n por nitr\u00f3geno en pseudomonas putida"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ana Herv\u00e1s Veguillas <\/strong><\/h2>\n<p>El objetivo de este proyecto de tesis es estudiar la regulaci\u00f3n por nitr\u00f3geno en la bacteria de suelo pseudomonas putida, un organismo modelo en el estudio de la biodegradaci\u00f3n de contaminantes org\u00e1nicos. Para ello, en una primera fase se ha hecho un estudio a nivel global, mediante el uso de microarrays de dna, de la respuesta de p. Putida a la disponibilidad de nitr\u00f3geno en el medio de cultivo y de c\u00f3mo afecta la p\u00e9rdida del gen ntrc, conocido regulador global de respuesta a nitr\u00f3geno en enterobacterias y presente tambi\u00e9n en el genoma de p. Putida, a la expresi\u00f3n de genes en condiciones de limitaci\u00f3n de nitr\u00f3geno. Los resultados de los microarrays han sido validados usando la t\u00e9cnica de pcr cuantitativa para medir la expresi\u00f3n de diversos genes. Una vez obtenida una visi\u00f3n general de la regulaci\u00f3n por nitr\u00f3geno, pasamos en una segunda fase a estudiar la regulaci\u00f3n por nitr\u00f3geno de genes de inter\u00e9s seleccionados del estudio por microarrays, para profundizar en los mecanismos moleculares de dicha regulaci\u00f3n y determinar las similitudes y diferencias que existen entre esta regulaci\u00f3n en enterobacterias y en p. Putida. As\u00ed, se determinaron los inicios de la transcripci\u00f3n, el tipo de promotor que opera en cada gen seleccionado, y si dependen directamente del regulador global ntrc. Para ello se utilizaron diversas t\u00e9cnicas tales como ensayos de actividad b-galactosidasa, extensi\u00f3n desde cebador, ensayos de protecci\u00f3n frente a digesti\u00f3n con dnasal, ensayos de formaci\u00f3n de complejo abierto y ensayos de transcripci\u00f3n in vitro.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Regulaci\u00f3n por nitr\u00f3geno en pseudomonas putida<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Regulaci\u00f3n por nitr\u00f3geno en pseudomonas putida <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ana Herv\u00e1s Veguillas <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pablo de olavide<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 22\/07\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Eduardo Santero Santurino<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: josep Casadesus pursals <\/li>\n<li>enrique Flores garcia (vocal)<\/li>\n<li>Manuel Carmona p\u00e9rez (vocal)<\/li>\n<li>asunci\u00f3n Contreras de vera (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ana Herv\u00e1s Veguillas El objetivo de este proyecto de tesis es estudiar la regulaci\u00f3n por nitr\u00f3geno en [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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