{"id":111574,"date":"2018-03-11T10:38:06","date_gmt":"2018-03-11T10:38:06","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/role-of-the-regulation-of-cell-lipid-composition-and-membrane-structure-in-the-antitumor-effect-of-2-hydroxyoelic-acid\/"},"modified":"2018-03-11T10:38:06","modified_gmt":"2018-03-11T10:38:06","slug":"role-of-the-regulation-of-cell-lipid-composition-and-membrane-structure-in-the-antitumor-effect-of-2-hydroxyoelic-acid","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/role-of-the-regulation-of-cell-lipid-composition-and-membrane-structure-in-the-antitumor-effect-of-2-hydroxyoelic-acid\/","title":{"rendered":"Role of the regulation of cell lipid composition and membrane structure in the antitumor effect of 2-hydroxyoelic acid."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Mar\u00eda Laura Mart\u00edn <\/strong><\/h2>\n<p>Resumen  el \u00e1cido 2-hidroxioleico (2ohoa) es un f\u00e1rmaco antitumoral dise\u00f1ado para regular la estructura y composici\u00f3n de los l\u00edpidos de membrana y la funci\u00f3n de importantes prote\u00ednas de membrana.  el objetivo principal de este trabajo fue estudiar c\u00f3mo el 2ohoa modula la composici\u00f3n lip\u00eddica y la estructura de membrana en las c\u00e9lulas tumorales. se observ\u00f3 que el 2ohoa indujo profundas alteraciones en el contenido de fosfol\u00edpidos, aumentando el contenido de esfingomielina y disminuyendo el contenido de fosfatidiletanolamina y fosfatidilcolina. Este efecto fue espec\u00edfico contra las c\u00e9lulas cancerosas, ya que el tratamiento no afect\u00f3 la composici\u00f3n lip\u00eddica de las c\u00e9lulas no tumorales mrc-5 de fibroblastos humanos. El aumento de sm se debi\u00f3 a una activaci\u00f3n r\u00e1pida y espec\u00edfica de las sm sintasas. Como consecuencia de la activaci\u00f3n sostenida de la sms, todo el metabolismo de los esfingol\u00edpidos se vio afectado. finalmente, se evalu\u00f3 el impacto de todos estos cambios sobre las propiedades biof\u00edsicas de membrana mediante espectroscopia de fluorescencia.   abstract  2-hydroxyoleic acid (2ohoa) is a potent antitumor drug that was designed to regulate membrane lipid composition and structure and the function of important membrane proteins.  the main goal of this work was to study how 2ohoa modulates the membrane lipid composition and structure of tumor cells.  2ohoa induced dramatic alterations in phospholipid content, increasing sphingomyelin mass, and decreasing phosphatidyl-ethanolamine and phosphatidylcholine.  this effect was specific against cancer cells as it did not affect non-tumor mrc-5 cells. The increased sm mass was due to a rapid and highly specific activation of sm synthases. As a consequence of the sustained activation of sms, the whole sphingolipid metabolism was affected. then, the impact of all these changes on membrane biophysical properties was evaluated by fluorescence spectroscopy<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Role of the regulation of cell lipid composition and membrane structure in the antitumor effect of 2-hydroxyoelic acid.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Role of the regulation of cell lipid composition and membrane structure in the antitumor effect of 2-hydroxyoelic acid. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Mar\u00eda Laura Mart\u00edn <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Illes balears<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/10\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Gwendolyn Barcel\u00f3 Coblijn<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: john e. Halver <\/li>\n<li>richard n. Kolesnick (vocal)<\/li>\n<li>rodrigo Freire  martins de almeida (vocal)<\/li>\n<li>eric james Murphy (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mar\u00eda Laura Mart\u00edn Resumen el \u00e1cido 2-hidroxioleico (2ohoa) es un f\u00e1rmaco antitumoral dise\u00f1ado para regular la estructura [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[849,17349,9,19034,1027],"tags":[222254,126156,222251,222250,222252,222253],"class_list":["post-111574","post","type-post","status-publish","format-standard","hentry","category-biologia-celular","category-biotecnologia","category-ciencias-medicas","category-illes-balears","category-oncologia","tag-eric-james-murphy","tag-gwendolyn-barcelo-coblijn","tag-john-e-halver","tag-maria-laura-martin","tag-richard-n-kolesnick","tag-rodrigo-freire-martins-de-almeida"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/111574","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=111574"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/111574\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=111574"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=111574"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=111574"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}