{"id":111719,"date":"2011-07-11T00:00:00","date_gmt":"2011-07-11T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/characterization-of-ul1-a-member-of-the-human-cytomegalovirus-rl11-gene-family\/"},"modified":"2011-07-11T00:00:00","modified_gmt":"2011-07-11T00:00:00","slug":"characterization-of-ul1-a-member-of-the-human-cytomegalovirus-rl11-gene-family","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/virologia\/characterization-of-ul1-a-member-of-the-human-cytomegalovirus-rl11-gene-family\/","title":{"rendered":"Characterization of ul1, a member of the human cytomegalovirus rl11 gene family"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Medya Shikhagaie <\/strong><\/h2>\n<p>We have investigated the previously uncharacterized human cytomegalovirus (hcmv) ul1 open  reading frame (orf), a member of the lately evolving hcmv rl11 gene family. Ul1 is hcmv specific;  absence of ul1 in chimpanzee cmv (ccmv) and sequence analysis studies suggests that ul1 may have  originated by duplication of an ancestor gene from the rl11-trl-cluster (trl11, trl12 and trl13).  sequence similarity searches against human immunoglobulin (ig) containing proteins revealed that hcmv  pul1 shows significant similarity to the cellular carcinoembrionic antigen related (cea) protein family n-  terminal ig domain, which is responsible for cea ligand recognition. Northern blot analysis revealed that  ul1 is transcribed during the late phase of the hcmv replication cycle, both in fibroblast-adapted and  endotheliotropic strains of hcmv. We characterized the protein encoded by an ha-tagged ul1 in the  ad169-derived hb5 background. Ul1 is expressed as a 224 amino acid type i transmembrane glycoprotein  which becomes detectable not earlier than 48 h to 72 h post infection. In infected human fibroblasts, pul1  co-localized at the cytoplasmic site of virion assembly and secondary envelopment together with tgn-46,  a marker for the trans-golgi network, and viral structural proteins including envelope glycoprotein b and  tegument phosphoprotein pp28. Furthermore, analysis of highly purified ad169 ul1-ha epitope tagged  virions revealed that pul1 is a novel constituent of the hcmv envelope. Importantly, deletion of ul1 in  hcmv tb40\/e resulted in a reduced growth in a cell type-specific manner, suggesting that pul1 may be  implicated in regulating hcmv cell tropism.  palabras clave de la tesis: cytomegalovirus, virion, cell tropism, glycoprotein, gene expression<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Characterization of ul1, a member of the human cytomegalovirus rl11 gene family<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Characterization of ul1, a member of the human cytomegalovirus rl11 gene family <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Medya Shikhagaie <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pompeu fabra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 07\/11\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Miguel Lopez Botet Arbona<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: pablo Engel rocamora <\/li>\n<li>Alberto Fraile ramos (vocal)<\/li>\n<li>  (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Medya Shikhagaie We have investigated the previously uncharacterized human cytomegalovirus (hcmv) ul1 open reading frame (orf), a [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[18712,280],"tags":[222516,222515,6524,34391],"class_list":["post-111719","post","type-post","status-publish","format-standard","hentry","category-pompeu-fabra","category-virologia","tag-alberto-fraile-ramos","tag-medya-shikhagaie","tag-miguel-lopez-botet-arbona","tag-pablo-engel-rocamora"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/111719","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=111719"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/111719\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=111719"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=111719"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=111719"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}