{"id":111865,"date":"2018-03-11T10:38:33","date_gmt":"2018-03-11T10:38:33","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/el-gen-7-de-alfacoronavirus-1-contrarresta-a-la-respuesta-huesped-y-modula-la-virulencia-del-virus\/"},"modified":"2018-03-11T10:38:33","modified_gmt":"2018-03-11T10:38:33","slug":"el-gen-7-de-alfacoronavirus-1-contrarresta-a-la-respuesta-huesped-y-modula-la-virulencia-del-virus","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-molecular\/el-gen-7-de-alfacoronavirus-1-contrarresta-a-la-respuesta-huesped-y-modula-la-virulencia-del-virus\/","title":{"rendered":"\u00abel gen 7 de alfacoronavirus 1 contrarresta a la respuesta hu\u00e9sped y modula la virulencia del virus\u00bb."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jazmina Libertad Gonz\u00e1lez Cruz <\/strong><\/h2>\n<p>Transmissible gastroenteritis virus (tgev) genome contains three accessory genes: 3a, 3b and 7. Gene 7 is only present in members of the species alphacoronavirus 1, and encodes a hydrophobic protein of 78 aa. To study gene 7 function, a recombinant tgev virus lacking gene 7 was engineered (rtgev-\u00c2\u00bf7). Both the deletion mutant and the parental (rtgev-wt) viruses showed the same growth and viral rna synthesis kinetics in tissue culture. Nevertheless, cells infected with rtgev-\u00c2\u00bf7 virus showed an increased cytopathic effect caused by an enhanced apoptosis mediated by caspase activation. analysis of macromolecular synthesis showed that rtgev-\u00c2\u00bf7 virus infection led to host translational shut-off and increased cellular rna degradation compared with rtgev-wt infection. An increase of eukaryotic translation initiation factor 2 (eif2\u00c2\u00bf) phosphorylation and an enhanced nuclease activity were observed in rtgev-\u00c2\u00bf7 virus infected cells. These results suggested that the removal of gene 7 promoted an intensified dsrna-activated host antiviral response. a conserved sequence motif that potentially mediates binding to protein phosphatase 1 catalytic subunit (pp1c), a key regulator of the cell antiviral defenses, was identified in protein 7 by bioinformatic analysis. In fact, pull-down assays demonstrated the interaction between pp1 and tgev protein 7, but not a protein 7 mutant lacking pp1c binding motif. Furthermore, eif2\u00c2\u00bf was also present in this complex, strongly suggesting a regulation of eif2\u00c2\u00bf phosphorylation by protein 7. Moreover, the interaction between protein 7 and pp1 was required for eif2a dephosphotylation and inhibition of cell rna degradation. These data indicated that tgev protein 7 counteracted host antiviral response by its association with pp1c. the analysis of the gene expression profiling in rtgev-wt and rtgev-\u00c2\u00bf7 infected cells using microarrays, showed that the genes differentially expressed were involved in response to virus and inflammation. These results were confirmed by rt-qpcr using specific taqman assays, suggesting that the absence of protein 7 during tgev infection led to enhanced levels of pro-inflammatory cytokines such as ifn\u00edY, tnf, rantes, ccl2 and ccl4. inoculation of newborn piglets with rtgev-\u00c2\u00bf7 and rtgev-wt viruses showed that rtgev-\u00c2\u00bf7 virus led to an accelerated growth kinetics and pathology compared with the parental virus. Furthermore, the increased pathology caused by the rtgev-\u00c2\u00bf7 infection could be due to and exacerbate macrophage activation, as a higher macrophage resumen en ingl\u00e9s 2 infiltration in lung was observed in the animals infected with the deletion mutant virus compared with those infected with the wild type. overall, the results indicated that gene 7 counteracted host cell defenses, and modified tgev virulence increasing tgev survival. Therefore, the acquisition of gene 7 by the tgev genome most likely has provided a selective advantage to the virus.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>\u00abel gen 7 de alfacoronavirus 1 contrarresta a la respuesta hu\u00e9sped y modula la virulencia del virus\u00bb.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 \u00abel gen 7 de alfacoronavirus 1 contrarresta a la respuesta hu\u00e9sped y modula la virulencia del virus\u00bb. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jazmina Libertad Gonz\u00e1lez Cruz <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 25\/11\/2011<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Luis EnJuan es Sanchez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda no Esteban rodriguez <\/li>\n<li>Jos\u00e9 Antonio Melero fondevilla (vocal)<\/li>\n<li>Francisco Javier Ortego alonso (vocal)<\/li>\n<li>isidoro Gonz\u00e1lez mart\u00ednez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jazmina Libertad Gonz\u00e1lez Cruz Transmissible gastroenteritis virus (tgev) genome contains three accessory genes: 3a, 3b and 7. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483],"tags":[191678,222755,222754,14396,5594,23995],"class_list":["post-111865","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","tag-francisco-javier-ortego-alonso","tag-isidoro-gonzalez-Martinez","tag-jazmina-libertad-gonzalez-cruz","tag-jose-antonio-melero-fondevilla","tag-luis-enjuan-es-sanchez","tag-maria-no-esteban-rodriguez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/111865","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=111865"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/111865\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=111865"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=111865"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=111865"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}