{"id":112676,"date":"2012-08-05T00:00:00","date_gmt":"2012-08-05T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-y-caracterizacion-de-las-celulas-madre-de-la-membrana-amniotica-una-nueva-fuente-para-terapia-celular-e-inmunomodulacion\/"},"modified":"2012-08-05T00:00:00","modified_gmt":"2012-08-05T00:00:00","slug":"aislamiento-y-caracterizacion-de-las-celulas-madre-de-la-membrana-amniotica-una-nueva-fuente-para-terapia-celular-e-inmunomodulacion","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/aislamiento-y-caracterizacion-de-las-celulas-madre-de-la-membrana-amniotica-una-nueva-fuente-para-terapia-celular-e-inmunomodulacion\/","title":{"rendered":"Aislamiento y caracterizaci\u00f3n de las c\u00e9lulas madre de la membrana amni\u00f3tica. una nueva fuente para terapia celular e inmunomodulaci\u00f3n"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Carmen Luisa Garcia De Insausti <\/strong><\/h2>\n<p>Se realiz\u00f3 el aislamiento y caracterizaci\u00f3n de las c\u00e9lulas de 20 membranas amni\u00f3ticas.  De cada ma se obtuvo un promedio de 123\u00c2\u00b112&#215;106 c\u00e9lulas epiteliales (haec) y 5.3&#215;106 c\u00e9lulas mesenquimales (hamsc). Las heac expresaron ssea (1, 3 y 4), y tra (1-60 y 1-81). Las hamsc expresaron cd73, cd90, cd105 y no cd34, cd45, cd14 ni cd19. Ambas fueron negativas para hla dr y positivas para transcriptos de nanog, oct-4 y sox-2.  En los cultivos primarios las haec mostraron menor capacidad de auto-renovaci\u00f3n y proliferaci\u00f3n que las hamsc, y en los sub-cultivos desarrollaron transici\u00f3n epitelio-mes\u00e9nquima. Todas las haec mostraron potencialidad para diferenciarse en c\u00e9lulas de las tres capas germinales (neuroectod\u00e9rmicas, hepatoc\u00edticas y cardiomioc\u00edticas), algunas para diferenciaci\u00f3n osteog\u00e9nica y adipog\u00e9nica, y ninguna para condrog\u00e9nica. Todas las hamsc se diferenciaron hacia l\u00edneas osteog\u00e9nicas, adipog\u00e9nicas y condrog\u00e9nicas.    en conclusi\u00f3n, la ma representa un reservorio de c\u00e9lulas madre pluripotenciales y multipotenciales inmunol\u00f3gicamente privilegiadas.       abstract:  in this study we determine the quantity and quality of amnion cells after isolation and culture from 20 placentas. Following our protocol, primary yields were 123 \u00c2\u00b112 x 106 haecs and 5.3&#215;106 hamscs. Flow cytometric characterization showed expression of ssea (1, 3 and 4) and tra (1-60 and 1-81) in haecs. Hamsc expressed cd73, cd90, cd105 and none cd45, cd34, cd14, cd19. Both cells types were negative for hla-dr and exhibited nanog, oct-4 and sox-2 transcripts. In primary cultures haecs showed slower proliferation and less clonogenic capacity than hamscs, in sub-cultures they exhibited epithelial mesenchymal transition. All haecs samples were induced to differentiate into neuroectodermal, hepatic and cardiomiocitic cells, while osteogenic y adipogenic capacity varied between them. All hamscs samples exhibited osteogenic, adipogenic and chondrogenic differentiation capacity.   in conclusion, term amnion may be a useful source of pluripotent and multipotent stem cells that possess a degree of immune privilege.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento y caracterizaci\u00f3n de las c\u00e9lulas madre de la membrana amni\u00f3tica. una nueva fuente para terapia celular e inmunomodulaci\u00f3n<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento y caracterizaci\u00f3n de las c\u00e9lulas madre de la membrana amni\u00f3tica. una nueva fuente para terapia celular e inmunomodulaci\u00f3n <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Carmen Luisa Garcia De Insausti <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 08\/05\/2012<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Mar\u00eda Moraleda Jim\u00e9nez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: salvador Mart\u00ednez p\u00e9rez <\/li>\n<li>augusto Silva gonzalez (vocal)<\/li>\n<li> Majado mart\u00ednez m. juliana (vocal)<\/li>\n<li>damian Garc\u00eda olmo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carmen Luisa Garcia De Insausti Se realiz\u00f3 el aislamiento y caracterizaci\u00f3n de las c\u00e9lulas de 20 membranas [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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