{"id":112882,"date":"2018-03-11T10:40:04","date_gmt":"2018-03-11T10:40:04","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/effects-of-hypoxia-on-mammary-stem-cells-and-breast-cancer-initiating-cells\/"},"modified":"2018-03-11T10:40:04","modified_gmt":"2018-03-11T10:40:04","slug":"effects-of-hypoxia-on-mammary-stem-cells-and-breast-cancer-initiating-cells","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/effects-of-hypoxia-on-mammary-stem-cells-and-breast-cancer-initiating-cells\/","title":{"rendered":"Effects of hypoxia on mammary stem cells and breast cancer initiating cells"},"content":{"rendered":"

Tesis doctoral de Oihana Iriondo Martinez De Zuazo <\/strong><\/h2>\n

El c\u00e1ncer de mama es el tipo de tumor m\u00e1s frecuente en mujeres, tanto en t\u00e9rminos de incidencia como de mortalidad. Por ello, el estudio en profundidad de la enfermedad es de suma importancia, ya que facilitar\u00e1 el desarrollo de terapias m\u00e1s eficaces e individualizadas. Las c\u00e9lulas madre adultas, caracterizadas por la capacidad de perpetuarse por autoregeneraci\u00f3n y generar los diferentes tipos celulares del tejido, son esenciales para el mantenimiento de la homeostasis tisular en varios \u00f3rganos. Adem\u00e1s, las c\u00e9lulas madre del c\u00e1ncer (cmc) pueden jugar un papel crucial en la iniciaci\u00f3n y el desarrollo de tumores. Tanto en tejido normal como en tejido canceroso, las c\u00e9lulas madre residen en microambientes complejos. En estos nichos, varios factores, incluyendo la reducida disponibilidad de ox\u00edgeno (hipoxia), pueden influenciar las propiedades de la c\u00e9lulas madre. Sin embargo, a\u00fan no se conoce c\u00f3mo influye la hipoxia sobre las propiedades de las c\u00e9lulas madre de la mama, ni en la mama sana, ni en los tumores mamarios.Con el objetivo de responder a esta pregunta, analizamos el efecto de la hipoxia sobre las c\u00e9lulas madre, tanto en c\u00e9lulas primarias del epitelio mamario como en l\u00edneas celulares de c\u00e1ncer de mama, en ambos casos, la hipoxia aument\u00f3 la proporci\u00f3n de c\u00e9lulas madre, definidas por la expresi\u00f3n combinada de marcadores de superficie celular, actividad elevada de aldh1 o mayor capacidad de formar mamoesferas. Descubrimos que los factores de la v\u00eda de se\u00f1alizaci\u00f3n de la hipoxia que regulan el incremento en cmc difieren seg\u00fan el fenotipo de c\u00e9lula madre analizado. En l\u00edneas celulares er\u00c2\u00bf-negativos, la hipoxia aument\u00f3 el porcentaje de c\u00e9lulas cd44+cd24-\/low por medio de un proceso de desdiferenciaci\u00f3n que, siendo independiente de hif, involucraba a phd3. Por el contrario, en c\u00e9lulas er\u00c2\u00bf positivas, hif1\u00c2\u00bf era necesario para incrementar el contenido de cmc en respuesta a hipoxia. Adem\u00e1s, en c\u00e9lulas er\u00c2\u00bf-positivas, mostramos que el estr\u00f3geno aumenta la expresi\u00f3n y actividad transcripcional de hif1\u00c2\u00bf de forma significativa, mientras que la hipoxia reduce la expresi\u00f3n y actividad transcripcional de er\u00c2\u00bf. En conclusi\u00f3n, estos hallazgos sugieren que el microambiente hip\u00f3xico promueve un fenotipo menos diferenciado en c\u00e9lulas epiteliales de la mama y en c\u00e9lulas de c\u00e1ncer de mama.<\/p>\n

 <\/p>\n

Datos acad\u00e9micos de la tesis doctoral \u00abEffects of hypoxia on mammary stem cells and breast cancer initiating cells<\/strong>\u00ab<\/h3>\n
    \n
  • T\u00edtulo de la tesis:<\/strong>\u00a0 Effects of hypoxia on mammary stem cells and breast cancer initiating cells <\/li>\n
  • Autor:<\/strong>\u00a0 Oihana Iriondo Martinez De Zuazo <\/li>\n
  • Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n
  • Fecha de lectura de la tesis:<\/strong>\u00a0 26\/06\/2012<\/li>\n<\/ul>\n

     <\/p>\n

    Direcci\u00f3n y tribunal<\/h3>\n
      \n
    • Director de la tesis<\/strong>\n
        \n
      • Mar\u00eda Del Mar Vivanco Ruiz<\/li>\n<\/ul>\n<\/li>\n
      • Tribunal<\/strong>\n
          \n
        • Presidente del tribunal: javier Le\u00f3n serrano <\/li>\n
        • f\u00c2\u00ba Javier Oliver pozo (vocal)<\/li>\n
        • spiros Linardopoulos — (vocal)<\/li>\n
        • Fernando Jos\u00e9 Unda rodriguez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          Tesis doctoral de Oihana Iriondo Martinez De Zuazo El c\u00e1ncer de mama es el tipo de tumor m\u00e1s frecuente en […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35,9,12909],"tags":[145496,143066,14452,196199,224398,224399],"class_list":["post-112882","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","category-ciencias-medicas","category-pais-vasco-euskal-herriko-unibertsitatea","tag-fao-javier-oliver-pozo","tag-fernando-jose-unda-rodriguez","tag-javier-leon-serrano","tag-maria-del-mar-vivanco-ruiz","tag-oihana-iriondo-Martinez-de-zuazo","tag-spiros-linardopoulos"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/112882","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=112882"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/112882\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=112882"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=112882"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=112882"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}