{"id":113017,"date":"2012-12-07T00:00:00","date_gmt":"2012-12-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-cinetica-de-la-catalisis-la-inactivacion-suicida-y-la-inhibicion-irreversible-de-tirosinasa\/"},"modified":"2012-12-07T00:00:00","modified_gmt":"2012-12-07T00:00:00","slug":"caracterizacion-cinetica-de-la-catalisis-la-inactivacion-suicida-y-la-inhibicion-irreversible-de-tirosinasa","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/caracterizacion-cinetica-de-la-catalisis-la-inactivacion-suicida-y-la-inhibicion-irreversible-de-tirosinasa\/","title":{"rendered":"Caracterizaci\u00f3n cin\u00e9tica de la cat\u00e1lisis, la inactivaci\u00f3n suicida y la inhibici\u00f3n irreversible de tirosinasa."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jos\u00e9 Luis Mu\u00f1oz Mu\u00f1oz <\/strong><\/h2>\n<p>En esta tesis doctoral se ha profundizado en el mecanismo cin\u00e9tico de actuaci\u00f3n de tirosinasa sobre distintas parejas monofenol\/o-difenol. A partir de los resultados obtenidos, se puso de manifiesto que la afinidad de la enzima por oxigeno es mayor en la ruta favorecida que en la ruta lenta. se ha demostrando cin\u00e9ticamente que desoxitirosinasa existe en dos formas: relajada y tensa (mayor distancia entre los \u00e1tomos de cobre). Gracias a este estudio, se han determinado dos pkas cr\u00edticos en tirosinasa. en relaci\u00f3n a la caracterizaci\u00f3n de sustratos de tirosinasa, se han desarrollado una serie de m\u00e9todos de medida que han permitido caracterizar una gran variedad de o-difenoles, trifenoles, flavonoides y compuestos no fen\u00f3licos como aminas arom\u00e1ticas y o-aminofenoles. por otra parte, se han realizado estudios cin\u00e9ticos sobre el proceso de inactivaci\u00f3n suicida que sufre tirosinasa cuando act\u00faa sobre sus sustratos proponiendo un mecanismo estructural que explica los resultados obtenidos.  palabras clave: bioqu\u00edmica (230200), bioqu\u00edmica molecular (230221), enzimolog\u00eda (230209).   in this doctoral thesis, we have depth on the kinetic mechanism of action of tyrosinase acting on different pairs of monophenol\/o-diphenol substrates. The results obtained revealed that the affinity of the enzyme for oxygen is greater in the kinetically preferred pathway than in the slow pathway. it has been demonstrated kinetically that deoxytyrosinase exists in two forms: relaxed and tense (greater distance between copper atoms). Thanks to this study, we have identified two critical pkas in tyrosinase. relating to the characterization of tyrosinase substrates, we have developed a series of measurement methods that allow the characterization of a variety of o-diphenols, triphenols, flavonoids and non-phenolic compounds such as aromatic amines and o-aminophenols. furthermore, kinetic studies have been conducted on the suicide inactivation process that tyrosinase suffers when acting on their substrates and a structural mechanism that explain the obtained results has been proposed.  keywords: biochemistry (230200), molecular biochemistry (230221), enzymology (230209).<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n cin\u00e9tica de la cat\u00e1lisis, la inactivaci\u00f3n suicida y la inhibici\u00f3n irreversible de tirosinasa.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n cin\u00e9tica de la cat\u00e1lisis, la inactivaci\u00f3n suicida y la inhibici\u00f3n irreversible de tirosinasa. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jos\u00e9 Luis Mu\u00f1oz Mu\u00f1oz <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 12\/07\/2012<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Francisco Garcia Canovas<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: manuel Acosta echeverria <\/li>\n<li>Francisco Garc\u00eda sevilla (vocal)<\/li>\n<li>Mar\u00eda Jos\u00e9 Garc\u00eda meseguer (vocal)<\/li>\n<li>manuela Garcia moreno (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jos\u00e9 Luis Mu\u00f1oz Mu\u00f1oz En esta tesis doctoral se ha profundizado en el mecanismo cin\u00e9tico de actuaci\u00f3n [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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