{"id":113034,"date":"2018-03-11T10:40:19","date_gmt":"2018-03-11T10:40:19","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/nuevos-materiales-eutectoides-en-el-sistema-fosfato-tricalcico-silicato-dicalcico-para-regeneracion-osea\/"},"modified":"2018-03-11T10:40:19","modified_gmt":"2018-03-11T10:40:19","slug":"nuevos-materiales-eutectoides-en-el-sistema-fosfato-tricalcico-silicato-dicalcico-para-regeneracion-osea","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/traumatologia\/nuevos-materiales-eutectoides-en-el-sistema-fosfato-tricalcico-silicato-dicalcico-para-regeneracion-osea\/","title":{"rendered":"Nuevos materiales eutectoides en el sistema fosfato tric\u00e1lcico-silicato dic\u00e1lcico para regeneraci\u00f3n \u00f3sea."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Francesca Cragnolini <\/strong><\/h2>\n<p>Adult human bone marrow mesenchymal stem cells (ahmscs) are multipotent stromal cells capable of osteogenic differentiation. Three eutectoid materials in the system dicalcium silicate-tricalcium phosphate were investigated as scaffolds for bone bioengineering: their ability to support cell growth and ahmsc osteogenic differentiation with or without osteogenic supplements. When biomaterials are placed in culture medium an apatitic layer forms on their surfaces, ahmscs are therefore able to adhere, but only ec2 strongly increments ahmsc proliferation. Ec1 and ec2 augment the expression of osteoblastic lineage typical genes extracellular matrix proteins, such as osteocalcin and osteopontin, are detected from 21th day of incubation with the three materials. At 21 days, mineralization occurs specially in samples incubated with ec2 with osteogenic medium. Moreover, the decrease of cd105 antigen expression in cells seeded on ec2 suggests differentiation. Ec2 seems to be the best candidate for further investigations concerning its application in bone tissue engineering.  key words: ahmscs, bone bioengineering, ceramic biomaterials, dicalcium silicate-tricalcium phosphate, osteoinduction.   las c\u00e9lulas mesenchymales adultas de medula \u00f3sea (ahmscs) son c\u00e9lulas multipotentes capaces de diferenciaci\u00f3n osteogenica. Tres materiales eutectiodes en el sistema dicalcio silicato-tricalcio fosfato se estudiaron como matrices para la bioingenier\u00eda del hueso: su habilidad para suportar el crecimiento celular y el diferenciamiento osteogenico de las ahmscs, con o sin suplementos osteogenicos. Cuando los biomateriales son puestos en medio de crecimiento, una capa de apatita se forma en su superficie, esta favorece la adhesi\u00f3n de las ahmscs, pero solo ec2 aument\u00f3 la proliferaci\u00f3n significativamente. Ec1 y ec2 aumentan la expresi\u00f3n de genes osteoblasticos. Las prote\u00ednas de la matriz extracelular, como osteopontina y osteonectina, se detectaron a partir del d\u00eda 21 para los tres biomateriales. A 21 d\u00edas hubo mineralizaci\u00f3n especialmente con el ec2 en medio osteogenico. La disminuci\u00f3n del anf\u00edgeno cd105 en las c\u00e9lulas sembradas con ec2 sugiere diferenciaci\u00f3n. Ec2 es el mejor candidato para ulteriores estudios de ingenier\u00eda tisular.   palabras clave: ahmscs, ingenier\u00eda tisular, biomateriales ceramicos, dicalcio silicato-tricalcio fosfato, osteoinducci\u00f3n.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Nuevos materiales eutectoides en el sistema fosfato tric\u00e1lcico-silicato dic\u00e1lcico para regeneraci\u00f3n \u00f3sea.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Nuevos materiales eutectoides en el sistema fosfato tric\u00e1lcico-silicato dic\u00e1lcico para regeneraci\u00f3n \u00f3sea. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Francesca Cragnolini <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/07\/2012<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Piedad N. De Aza Moya<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: vicente Vicente ortega <\/li>\n<li>pablo Andr\u00e9s Velasquez castillo (vocal)<\/li>\n<li>Fernando L\u00f3pez prats (vocal)<\/li>\n<li>Miguel \u00e1ngel Rodr\u00edguez barbero (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Francesca Cragnolini Adult human bone marrow mesenchymal stem cells (ahmscs) are multipotent stromal cells capable of osteogenic [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[17349,8235,254],"tags":[40642,224636,146969,224638,224637,8486],"class_list":["post-113034","post","type-post","status-publish","format-standard","hentry","category-biotecnologia","category-murcia","category-traumatologia","tag-fernando-lopez-prats","tag-francesca-cragnolini","tag-miguel-angel-rodriguez-barbero","tag-pablo-andres-velasquez-castillo","tag-piedad-n-de-aza-moya","tag-vicente-vicente-ortega"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/113034","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=113034"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/113034\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=113034"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=113034"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=113034"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}