{"id":113093,"date":"2018-03-11T10:40:24","date_gmt":"2018-03-11T10:40:24","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/muestreo-y-analisis-del-transcriptoma-de-melon-cucumis-melo-l\/"},"modified":"2018-03-11T10:40:24","modified_gmt":"2018-03-11T10:40:24","slug":"muestreo-y-analisis-del-transcriptoma-de-melon-cucumis-melo-l","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/virus-de-las-plantas\/muestreo-y-analisis-del-transcriptoma-de-melon-cucumis-melo-l\/","title":{"rendered":"Muestreo y an\u00e1lisis del transcriptoma de mel\u00f3n (cucumis melo l.)"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Daniel Gonzalez Ibeas <\/strong><\/h2>\n<p>El mel\u00f3n es uno de los cultivos m\u00e1s importantes del mundo. Las infecciones por virus son una de las principales causas de p\u00e9rdidas en la producci\u00f3n, mayoritariamente las causadas por virus de rna. El muestreo de los transcritos de rna (transcriptoma) que se generan a partir del genoma proporciona un abordaje funcional al contenido gen\u00e9tico de una especie. En esta tesis se aporta un an\u00e1lisis de dos porciones del transcriptoma de mel\u00f3n: la que corresponde a transcritos que codifican prote\u00ednas mediante un microarray de dna, y la que corresponde a peque\u00f1os rnas no codificantes mediante secuenciaci\u00f3n masiva. Se ha analizado el transcriptoma en respuesta a la infecci\u00f3n por el virus del mosaico de la sand\u00eda en un genotipo de mel\u00f3n resistente y otro susceptible al virus. Adem\u00e1s, se ha optimizado un m\u00e9todo de s\u00edntesis de cdna para hibridaciones en microarray. Se ha optado por presentar la tesis en modo de compendio de tres publicaciones.  palabras clave: mel\u00f3n, genotecas, cdna, microarray, pirosecuenciaci\u00f3n, ests, peque\u00f1os rnas,  virus, transcript\u00f3mica  abstract.  melon is one of the most important horticultural crops worldwide. Plant viral diseases cause serious economic losses by reducing yield and quality. Sampling of the complete set of cellular rna transcripts (transcriptome) has been used as an effective method for dissecting genetic content of many species and for analyzing several biological processes. In this thesis, two parts of the melon transcriptome have been analyzed: protein-coding transcripts by means of a dna microarray, and small rnas by means of pyrosequencing. Transcriptomic changes in response to watermelon mosaic virus infection have been monitored in a resistant and a susceptible melon genotype. In addition, a method for cdna synthesis for microarray hybridizations has been optimized. Results are presented as a compilation of three publications.  key words: melon, cdna libraries, cdna, microarray, pyrosequencing, ests, small rnas, virus, transcriptomic<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Muestreo y an\u00e1lisis del transcriptoma de mel\u00f3n (cucumis melo l.)<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Muestreo y an\u00e1lisis del transcriptoma de mel\u00f3n (cucumis melo l.) <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Daniel Gonzalez Ibeas <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 23\/07\/2012<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Ver\u00f3nica Truniger Rietmann<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: victoriano Valpuesta fernandez <\/li>\n<li>Mar\u00eda  belen Pico sirvent (vocal)<\/li>\n<li>Francisco borja Flores pardo (vocal)<\/li>\n<li>Miguel angel Botella mesa (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Daniel Gonzalez Ibeas El mel\u00f3n es uno de los cultivos m\u00e1s importantes del mundo. Las infecciones por [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[37372,10792,45144,8235,5219],"tags":[224732,43513,69401,7879,223757,2344],"class_list":["post-113093","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular-de-plantas","category-fitopatologia","category-genetica-molecular-de-plantas","category-murcia","category-virus-de-las-plantas","tag-daniel-gonzalez-ibeas","tag-francisco-borja-flores-pardo","tag-maria-belen-pico-sirvent","tag-miguel-angel-botella-mesa","tag-veronica-truniger-rietmann","tag-victoriano-valpuesta-fernandez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/113093","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=113093"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/113093\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=113093"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=113093"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=113093"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}