{"id":113262,"date":"2012-01-10T00:00:00","date_gmt":"2012-01-10T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-electrofisiologico-de-la-selectividad-del-transportador-de-nucleosidos-hcnt2-y-de-las-propiedades-electrogenicas-de-los-transportadores-de-acidos-biliares-oatp1b1-y-oatp1b3\/"},"modified":"2012-01-10T00:00:00","modified_gmt":"2012-01-10T00:00:00","slug":"estudio-electrofisiologico-de-la-selectividad-del-transportador-de-nucleosidos-hcnt2-y-de-las-propiedades-electrogenicas-de-los-transportadores-de-acidos-biliares-oatp1b1-y-oatp1b3","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/fisiologia-humana\/estudio-electrofisiologico-de-la-selectividad-del-transportador-de-nucleosidos-hcnt2-y-de-las-propiedades-electrogenicas-de-los-transportadores-de-acidos-biliares-oatp1b1-y-oatp1b3\/","title":{"rendered":"Estudio electrofisiol\u00f3gico de la selectividad del transportador de nucle\u00f3sidos hcnt2 y de las propiedades electrog\u00e9nicas de los transportadores de \u00e1cidos biliares oatp1b1 y oatp1b3"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Carlos Sancho Mateo <\/strong><\/h2>\n<p>University of navarra faculty of sciences electrophysiological study of the nucleoside transporter hcnt2  selectivity and of the electrogenic properties of the bile acid transporters oatp1b1 and oatp1b3 carlos sancho mateo 2012 \tthe human concentrative nucleoside transporter 2 (hcnt2), is a na+ dependent concentrative nucleoside cotransporter selective for purine nucleosides and urdine that can also transport nucleoside derivatives used in antiviral and anticancer therapies. In the present work, we have expressed hcnt2 in xenopus laevis oocytes, and used the two-electrodes voltage-clamp technique to study its interaction with a series of uridine and adenosine derivatives used in therapeutic treatments and synthesized in our university. The comparison of the kinetic parameters of the derivatives transported by hcnt2 with those corresponding to the transport by hcnt3 and hcnt1 demonstrates that hcnt2 is the most restrictive transporter among the human isoforms. It is also most restrictive that rat cnt2. The differences on the structural requirements of rat cnt2 and human cnt2 have implication on the experimental model used for the studies about the interaction of the transporters with drugs. \tusing the same technique, we have also studied the electrogenic properties of the human organic anion transporter polypeptides type 1 and type 3, oatp1b1 and oatp1b3. We demonstrate for the first time that these transporters are electrogenic showing two types of currents: a current associated to the transport of the substrates and a na+ current uncoupled to the substrate transport (na+ leak current). These properties will allow the study of the functional mechanism of these transporters using electrophysiological methods.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio electrofisiol\u00f3gico de la selectividad del transportador de nucle\u00f3sidos hcnt2 y de las propiedades electrog\u00e9nicas de los transportadores de \u00e1cidos biliares oatp1b1 y oatp1b3<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio electrofisiol\u00f3gico de la selectividad del transportador de nucle\u00f3sidos hcnt2 y de las propiedades electrog\u00e9nicas de los transportadores de \u00e1cidos biliares oatp1b1 y oatp1b3 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Carlos Sancho Mateo <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/10\/2012<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda Del Pilar Lostao Crespo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: cecilio Gimenez martin <\/li>\n<li>mar\u00ed\u00a7al Pastor anglada (vocal)<\/li>\n<li>oscar Briz s\u00e1nchez (vocal)<\/li>\n<li>Ana Barber c\u00e1rcamo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carlos Sancho Mateo University of navarra faculty of sciences electrophysiological study of the nucleoside transporter hcnt2 selectivity [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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