{"id":113508,"date":"2012-03-12T00:00:00","date_gmt":"2012-03-12T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/recognition-of-cng-repetitive-rnas-associated-with-human-diseases-by-rna-silencing-suppressor-p19-as-studied-by-x-ray-crystallography\/"},"modified":"2012-03-12T00:00:00","modified_gmt":"2012-03-12T00:00:00","slug":"recognition-of-cng-repetitive-rnas-associated-with-human-diseases-by-rna-silencing-suppressor-p19-as-studied-by-x-ray-crystallography","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/recognition-of-cng-repetitive-rnas-associated-with-human-diseases-by-rna-silencing-suppressor-p19-as-studied-by-x-ray-crystallography\/","title":{"rendered":"Recognition of cng-repetitive rnas associated with human diseases by rna silencing suppressor p19 as studied by x-ray crystallography"},"content":{"rendered":"

Tesis doctoral de Jevgenia Tamjar — <\/strong><\/h2>\n

Expanded tracts of cng-triplet repetitive sequences in human genome cause many diseases. We think that repeats cause diseases through rna silencing pathway (rnai) due to formation of the double helical rnas, containing mismatches at each third position. To be recognized by rnai machinery, such rnas have to be structurally similar to the canonic a-rna helix. To test if all cng-repetitive rnas form a-rna double helices that can be easily recognized by an a-rna binding protein and explore structural behavior of these double-helices upon variation of mismatches, strand-alignments and cng-repeat regularity, multiple crystal structure determinations have been carried out. Various cng-repetitive rnas, designed to contain all possible mismatches na\u00c2\u00bfnb in double-helical structure, were studied by x-ray crystallography in complex with rna-silencing suppressor p19 that is known to bind rna duplexes of a 19 base pairs length in a sequence unspecific way. Comparative structural analysis of the p19\/rna complexes elucidated the important role of the cng-triplet repetition in the rna sequences for the double helical structure stability. In turn, the `structural\u00c2\u00bf adaptability of the cng-repetitive rnas seems more dependable on mismatch pairs. The second part of the work deals with the attempt to find and introduce mutations into p19 that would allow it to recognize one of the cng-triplets in cng-repetitive rna duplex. For the first time, i) the structural behavior of various cng-repetitive rnas was studied upon complex-formation with an a-rna binding protein and ii) rna sequence specific mutations of p19 were structurally described.<\/p>\n

 <\/p>\n

Datos acad\u00e9micos de la tesis doctoral \u00abRecognition of cng-repetitive rnas associated with human diseases by rna silencing suppressor p19 as studied by x-ray crystallography<\/strong>\u00ab<\/h3>\n
    \n
  • T\u00edtulo de la tesis:<\/strong>\u00a0 Recognition of cng-repetitive rnas associated with human diseases by rna silencing suppressor p19 as studied by x-ray crystallography <\/li>\n
  • Autor:<\/strong>\u00a0 Jevgenia Tamjar — <\/li>\n
  • Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n
  • Fecha de lectura de la tesis:<\/strong>\u00a0 03\/12\/2012<\/li>\n<\/ul>\n

     <\/p>\n

    Direcci\u00f3n y tribunal<\/h3>\n
      \n
    • Director de la tesis<\/strong>\n
        \n
      • Lioudmila Malinina<\/li>\n<\/ul>\n<\/li>\n
      • Tribunal<\/strong>\n
          \n
        • Presidente del tribunal: felix Mar\u00eda Go\u00f1i urcelay <\/li>\n
        • miquel Coll capella (vocal)<\/li>\n
        • Juan Antonio Subirana torrent (vocal)<\/li>\n
        • armando Albert de la cruz (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          Tesis doctoral de Jevgenia Tamjar — Expanded tracts of cng-triplet repetitive sequences in human genome cause many diseases. We think […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[115,29842,12909],"tags":[14264,12081,225376,117,97165,26322],"class_list":["post-113508","post","type-post","status-publish","format-standard","hentry","category-bioquimica-molecular","category-espectroscopia-de-rayos-x","category-pais-vasco-euskal-herriko-unibertsitatea","tag-armando-albert-de-la-cruz","tag-felix-maria-goni-urcelay","tag-jevgenia-tamjar","tag-juan-antonio-subirana-torrent","tag-lioudmila-malinina","tag-miquel-coll-capella"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/113508","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=113508"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/113508\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=113508"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=113508"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=113508"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}