{"id":113784,"date":"2013-01-02T00:00:00","date_gmt":"2013-01-02T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-del-origen-desarrollo-y-capacidad-de-diferenciacion-de-las-celulas-precursoras-de-piel-humana-adulta\/"},"modified":"2013-01-02T00:00:00","modified_gmt":"2013-01-02T00:00:00","slug":"analisis-del-origen-desarrollo-y-capacidad-de-diferenciacion-de-las-celulas-precursoras-de-piel-humana-adulta","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica\/analisis-del-origen-desarrollo-y-capacidad-de-diferenciacion-de-las-celulas-precursoras-de-piel-humana-adulta\/","title":{"rendered":"An\u00e1lisis del origen, desarrollo y capacidad de diferenciaci\u00f3n de las c\u00e9lulas precursoras de piel humana adulta"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Adrian Perez San Vicente <\/strong><\/h2>\n<p>Resident neural precursor cells (npcs) have been reported for a number of adult tissues. Understanding their physiological function or, alternatively, their activation after tissue damage or in vitro manipulation remains an unsolved issue. Skin-derived precursor cells (skps) were first described as multipotent, neural crest-derived dermal cells of uncertain physiological function that generated neural and mesodermal progenies. Later it was postulated that hair follicle-associated sox2 dermal stem cells give rise to skps. Here we investigated the origin of human dermal npcs in the adult. By following an unbiased, comprehensive approach of cell surface marker screening, cell separation, transcriptomic characterisation and in vivo fate analyses, we found that sox2+ dermal stem\/precursor cells of human trunk skin can be traced back to the schwann cell and pericyte lineages, which seem to be developmentally interrelated. Moreover, neural differentiation of dermal stem\/precursor cells was restricted (>100-fold enrichment) to the schwann-like cells. We postulate a model by which neural competence of connective tissue stem\/precursor cells is dependent on sox gene expression levels. We further hypothesize that sox2+ resident precursors arise by dedifferentiation of schwann cells at peripheral nerve endings of multiple organs.En los \u00faltimos a\u00f1os se han publicado numerosos art\u00edculos que describen c\u00e9lulas precursoras neurales residentes (npc) en varios tejidos adultos no neurales. En la actualidad, se desconoce el rol fisiol\u00f3gico de estas c\u00e9lulas y si sus propiedades reflejan propiedades intr\u00ednsecas in situ o, alternativamente, se han adquirido en cultivo. Las c\u00e9lulas precursoras de la piel (skps) se describieron por primera vez como c\u00e9lulas d\u00e9rmicas multipotentes derivadas de la cresta neural de funci\u00f3n desconocida. Posteriormente, se estableci\u00f3 una relaci\u00f3n directa entre las c\u00e9lulas troncales d\u00e9rmicas sox2 positivas (residentes en la papila d\u00e9rmica del fol\u00edculo piloso) y las skps. El trabajo experimental presentado en esta tesis ha investigado el origen de las npcs dermales humanas en el adulto. Tras el an\u00e1lisis no sesgado de identificaci\u00f3n de linajes celulares determinando la expresi\u00f3n de 242 prote\u00ednas de superficie, fraccionamiento celular (cell sorting), caracterizaci\u00f3n transcript\u00f3mica y an\u00e1lisis de migraci\u00f3n in vivo, se ha demostrado que las c\u00e9lulas troncales\/precursoras sox2+ presentes en piel humana del tronco est\u00e1n asociadas a los linajes de las c\u00e9lulas de schwann y los pericitos, linajes que podr\u00edan estar interrelacionados. Adem\u00e1s, la subpoblaci\u00f3n asociada al linaje de schwann es la \u00fanica responsable de la capacidad de diferenciaci\u00f3n a linaje neural mostrada por las skps (incremento >100 veces). Proponemos un modelo unificado por el que la desdiferenciaci\u00f3n de las c\u00e9lulas de schwann en las terminaciones nerviosas perif\u00e9ricas, provocada por da\u00f1o tisular o manipulaci\u00f3n de las mismas, induce la competencia neural de precursores residentes preexistentes en el tejido conectivo de m\u00faltiples \u00f3rganos.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis del origen, desarrollo y capacidad de diferenciaci\u00f3n de las c\u00e9lulas precursoras de piel humana adulta<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis del origen, desarrollo y capacidad de diferenciaci\u00f3n de las c\u00e9lulas precursoras de piel humana adulta <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Adrian Perez San Vicente <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/02\/2013<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Ander Izeta Permisan<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Carlos Vicario abejon <\/li>\n<li>Jos\u00e9 Manuel Garcia verdugo (vocal)<\/li>\n<li>ashwin Woodhoo &#8212; (vocal)<\/li>\n<li>angel Raya chamorro (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Adrian Perez San Vicente Resident neural precursor cells (npcs) have been reported for a number of adult [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[849,160,452,12909],"tags":[225820,182009,27476,225821,40436,16726],"class_list":["post-113784","post","type-post","status-publish","format-standard","hentry","category-biologia-celular","category-bioquimica","category-embriologia-humana","category-pais-vasco-euskal-herriko-unibertsitatea","tag-adrian-perez-san-vicente","tag-ander-izeta-permisan","tag-angel-raya-chamorro","tag-ashwin-woodhoo","tag-carlos-vicario-abejon","tag-jose-manuel-garcia-verdugo"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/113784","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=113784"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/113784\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=113784"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=113784"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=113784"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}