{"id":113928,"date":"2013-08-03T00:00:00","date_gmt":"2013-08-03T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/inmovilizacion-de-tirosinasa-sobre-esteres-cinamicos-de-carbohidratos-fotoentrecruzados-caracterizacion-optimizacion-y-aplicacion-a-la-obtencion-de-o-difenoles\/"},"modified":"2013-08-03T00:00:00","modified_gmt":"2013-08-03T00:00:00","slug":"inmovilizacion-de-tirosinasa-sobre-esteres-cinamicos-de-carbohidratos-fotoentrecruzados-caracterizacion-optimizacion-y-aplicacion-a-la-obtencion-de-o-difenoles","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/inmovilizacion-de-tirosinasa-sobre-esteres-cinamicos-de-carbohidratos-fotoentrecruzados-caracterizacion-optimizacion-y-aplicacion-a-la-obtencion-de-o-difenoles\/","title":{"rendered":"Inmovilizaci\u00f3n de tirosinasa sobre \u00e9steres cin\u00e1micos de carbohidratos fotoentrecruzados. caracterizaci\u00f3n, optimizaci\u00f3n y aplicaci\u00f3n  a la obtenci\u00f3n de o-difenoles."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Mar\u00eda  Elisa Marin Zamora <\/strong><\/h2>\n<p>Resumen se ha desarrollado un nuevo m\u00e9todo de inmovilizaci\u00f3n de tirosinasa de champi\u00f1\u00f3n, basado en la adsorci\u00f3n de esta enzima a un \u00e9ster cin\u00e1mico de un carbohidrato fotoentrecruzado, con muy buenos resultados. Se han obtenido inmovilizados de tirosinasa directamente a partir de extractos de champi\u00f1\u00f3n. Se han estudiado los par\u00e1metros que afectan a la inmovilizaci\u00f3n (ph, tiempo y concentraci\u00f3n de enzima), las caracter\u00edsticas de tirosinasa inmovilizada (cantidad de enzima inmovilizada, actividad enzim\u00e1tica, ph y temperatura \u00f3ptimos de reacci\u00f3n, estabilidad t\u00e9rmica, operacional y al almacenado), las propiedades cin\u00e9ticas (constante de michaelis, velocidad m\u00e1xima y constante catal\u00edtica) de tirosinasa inmovilizada sobre distintos soportes al actuar sobre diversos sustratos y la estereoespecificidad de tirosinasa inmovilizada sobre un soporte quiral y otro no quiral respecto a los is\u00f3meros l y d y rac\u00e9micos. Los conocimientos adquiridos se han aplicado con \u00e9xito a la producci\u00f3n de diversos o-difenoles como l-dopa, a partir de sus correspondientes monofenoles. palabras clave: tirosinasa, inmovilizaci\u00f3n, enzima inmovilizada, extracci\u00f3n de tirosinasa, propiedades catal\u00edticas, \u00e9steres cin\u00e1micos, carbohidratos, o-difenols.  abstract a new mushroom tyrosinase immobilization method consisting of tyrosinase adsorption on a photocrosslinked cinnamic carbohydrate ester has been developed with very good results. Immobilized tyrosinase was obtained directly from a mushroom extract. We studied the parameters affecting the immobilization process (ph, time and enzyme concentration), the properties of immobilized tyrosinase (quantity of immobilized enzyme, enzymatic activity, optimum ph and reaction temperature; thermal, operational and storage stability), kinetic properties (michaelis constant, maximum steady-state rate and catalytic constant) of tyrosinase immobilized on several supports and acting over several substrates, and tyrosinase stereospecificity immobilized on a chiral and a nonchiral support acting on l and d isomers and racemics. The knowledge acquired was used to successfully produce several o-diphenols, such as l-dopa, from the corresponding monophenols. key words: tyrosinase, immobilization, immobilized enzyme, tyrosinase extraction, catalytic properties, cinnamic esters, carbohydrates, o-diphenols.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Inmovilizaci\u00f3n de tirosinasa sobre \u00e9steres cin\u00e1micos de carbohidratos fotoentrecruzados. caracterizaci\u00f3n, optimizaci\u00f3n y aplicaci\u00f3n  a la obtenci\u00f3n de o-difenoles.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Inmovilizaci\u00f3n de tirosinasa sobre \u00e9steres cin\u00e1micos de carbohidratos fotoentrecruzados. caracterizaci\u00f3n, optimizaci\u00f3n y aplicaci\u00f3n  a la obtenci\u00f3n de o-difenoles. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Mar\u00eda  Elisa Marin Zamora <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 08\/03\/2013<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Pedro Antonio Garcia Ruiz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: fructuoso Barba valiente <\/li>\n<li>ram\u00f3n Var\u00f3n castellanos (vocal)<\/li>\n<li>manuela Garcia moreno (vocal)<\/li>\n<li>Antonio Guirado moya (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mar\u00eda Elisa Marin Zamora Resumen se ha desarrollado un nuevo m\u00e9todo de inmovilizaci\u00f3n de tirosinasa de champi\u00f1\u00f3n, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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