{"id":114004,"date":"2018-03-11T10:41:48","date_gmt":"2018-03-11T10:41:48","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-del-gen-atc1-de-candida-parapsilosis-clonacion-estudio-fenota%c2%adpico-e-interaccion-con-el-sistema-inmunitario\/"},"modified":"2018-03-11T10:41:48","modified_gmt":"2018-03-11T10:41:48","slug":"caracterizacion-del-gen-atc1-de-candida-parapsilosis-clonacion-estudio-fenota%c2%adpico-e-interaccion-con-el-sistema-inmunitario","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/microbiologia-clinica\/caracterizacion-del-gen-atc1-de-candida-parapsilosis-clonacion-estudio-fenota%c2%adpico-e-interaccion-con-el-sistema-inmunitario\/","title":{"rendered":"Caracterizaci\u00f3n del gen atc1 de candida parapsilosis: clonaci\u00f3n, estudio fenot\u00edpico e interacci\u00f3n con el sistema inmunitario"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ruth Sanchez-fresneda Pinto <\/strong><\/h2>\n<p>Candida parapsilosis is the second most frequently isolated pathogenic yeast in blood from southamerica, asia and europe. The enzymes involved in the metabolism of trehalose, have been proposed as an antifungal target because this disaccharide acts against various types of stress in fungi but it is not present in mammals. here, we have cloned the gene encoding acid trehalase (atc1) in c. Parapsilosis and performed the phenotypic analysis of the null mutants. We studied the interaction of the obtained strains with the immune system. The homozygous mutant is unable to grow on trehalose and displays greater resistance to thermal, saline and oxidative stress in vitro. The atc1 gene disruption increased nitric oxide, tnf-? And il-10 cytokine production by human macrophages, and decreased cell survival to macrophage killing. These results are consistent with those obtained in vivo, where mutants also showed lower invasiveness in murine model and reduced virulence in galleria mellonella.  keywords: candida parapsilosis, atc1 gene, virulence, cytokine, trehalose.  candida parapsilosis es la segunda levadura pat\u00f3gena aislada en hemocultivos de hispanoam\u00e9rica latina, asia y europa. Las enzimas implicadas en el metabolismo de  trehalosa pueden servir como dianas antifungicas, ya que dicho disac\u00e1rido act\u00faa frente a varios tipos de estr\u00e9s en hongos y adem\u00e1s no se encuentra  presente en mam\u00edferos. hemos clonado el gen que codifica para la trehalasa \u00e1cida de c. Parapsilosis, realizando un an\u00e1lisis fenot\u00edpico del mutante nulo y de su interacci\u00f3n con el sistema inmunitario. El mutante homocig\u00f3tico es incapaz de crecer con trehalosa y presenta elevada resistencia al estr\u00e9s t\u00e9rmico, salino y oxidativo in vitro. La interrupci\u00f3n del gen atc1 incrementa los niveles de oxido n\u00edtrico y la producci\u00f3n de las citoquinas; tnf-? Y il-10, mientras reduce la supervivencia celular a lisis en macr\u00f3fagos humanos. Estos resultados coinciden con los obtenidos in vivo, donde los mutantes presentan una menor invasividad en modelo de rat\u00f3n y p\u00e9rdida de virulencia en galleria mellonella. palabras clave: candida parapsilosis, gen atc1, virulencia, citoquinas, trehalosa.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n del gen atc1 de candida parapsilosis: clonaci\u00f3n, estudio fenot\u00edpico e interacci\u00f3n con el sistema inmunitario<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n del gen atc1 de candida parapsilosis: clonaci\u00f3n, estudio fenot\u00edpico e interacci\u00f3n con el sistema inmunitario <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ruth Sanchez-fresneda Pinto <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 22\/03\/2013<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Eulogio Valent\u00edn G\u00f3mez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco Solano mu\u00f1oz <\/li>\n<li>emilia Matallana redondo (vocal)<\/li>\n<li>oscar Zaragoza hernandez (vocal)<\/li>\n<li>Mar\u00eda francisca Colom valiente (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ruth Sanchez-fresneda Pinto Candida parapsilosis is the second most frequently isolated pathogenic yeast in blood from southamerica, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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