{"id":114200,"date":"2018-03-11T10:42:05","date_gmt":"2018-03-11T10:42:05","guid":{"rendered":""},"modified":"2018-03-11T10:42:05","modified_gmt":"2018-03-11T10:42:05","slug":"identificacion-y-caracterizacion-genetica-de-la-carbapenemasa-oxa-58-en-aislamientos-cla%c2%adnicos-de-acinetobacter-baumannii-resistentes-a-imipenem-procedentes-de-hospitales-de-cochabamba-bolivia","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bacteriologia\/identificacion-y-caracterizacion-genetica-de-la-carbapenemasa-oxa-58-en-aislamientos-cla%c2%adnicos-de-acinetobacter-baumannii-resistentes-a-imipenem-procedentes-de-hospitales-de-cochabamba-bolivia\/","title":{"rendered":"Identificaci\u00f3n y caracterizaci\u00f3n gen\u00e9tica de la carbapenemasa oxa-58 en aislamientos cl\u00ednicos de acinetobacter baumannii resistentes a imipenem procedentes de hospitales de cochabamba, bolivia"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elena Fernandez Colon <\/strong><\/h2>\n<p>La emergencia de a. Baumannii como importante pat\u00f3geno nosocomial ha coincidido con el incremento de cepas resistentes a m\u00faltiples clases de antibi\u00f3ticos, incluyendo la \u00faltima alternativa terape\u00fatica, los carbapenems. En la \u00faltima d\u00e9cada el aislamiento de este tipo de cepas hab\u00eda sido espor\u00e1dico, pero actualmente en algunos pa\u00edses europeos aparecen cepas end\u00e9micas. Este estudio se llev\u00f3 a cabo en hospitales de la ciudad de cochabamba, bolivia,  pa\u00eds del que existe escasa informaci\u00f3n sobre este tema. El estudio incluye 46 aislamientos en los que se determin\u00f3 la sensibilidad antibi\u00f3tica mediante la t\u00e9cnica disco-placa y concentraci\u00f3n m\u00ednima inhibitoria (cmi). La relaci\u00f3n clonal se llev\u00f3 a cabo mediante pcr-fingerprinting y electroforesis en campos pulsados (pfge) con apa i. La detecci\u00f3n de carbapenemasas se realiz\u00f3 mediante t\u00e9cnicas fenot\u00edpicas y genot\u00edpicas. Las fenot\u00edpicas fueron: test de hodge, hodge+znso4, test de doble disco (ddst) y tambi\u00e9n se estudiaron betalactamasas de amplio espectro. Las genot\u00edpicas fueron mediante multiplex pcr para la detecci\u00f3n de genes blaoxa-23, blaoxa-40, blaoxa-51, blaoxa-58 y blaoxa-143. Se llev\u00f3 a cabo la secuenciaci\u00f3n de los genes blaoxa-69 y blaoxa-58.Tambi\u00e9n se analiz\u00f3 la presencia de estructuras gen\u00e9ticas m\u00f3viles: integrones de clase 1, secuencias de inserci\u00f3n isaba  1, 2 y 3, y pl\u00e1smidos..  Mediante el aislamiento de adn plasm\u00eddico e hibridaci\u00f3n con una sonda espec\u00edfica para el gen blaoxa-58, se detect\u00f3 la presencia de esta enzima dentro del pl\u00e1smido pab-58, de 40 kb. Adem\u00e1s, se realizaron experimentos adicionales para identificar m\u00faltiples mecanismos de resistencia en el clon a, genes que confieren resistencia a aminoglus\u00f3idos, quinolonas, a cefalosporinas y si conten\u00edan la bomba de expulsi\u00f3n ade-abc. Los resultados muestran un clon a multirresistente, \u00fanicamente sensible a colistina, portador de integrones, del pl\u00e1smido pab-58, y de las carbapenemasas oxa-58 y oxa-69, tambi\u00e9n del gen blaadc, de enzimas inactivantes de aminoglic\u00f3sidos, mutaciones en genes gyra y parc.  Adem\u00e1s, presentaban la bomba ade-abc sobreexpresada y secuencias de inserci\u00f3n isaba1 e isaba3 en los extremos de los genes blaadc y blaoxa-58, respectivamente, implicadas en la sobreexpresi\u00f3n de dichos genes. Todo esto indica que el clon a presenta una multitud de mecanismos de resistencia, algunos localizados en estructuras gen\u00e9ticas m\u00f3viles, y que su diseminaci\u00f3n puede ocasionar un grave problema a nivel hospitalario y de salud p\u00fablica en la ciudad de cochabamba, bolivia.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Identificaci\u00f3n y caracterizaci\u00f3n gen\u00e9tica de la carbapenemasa oxa-58 en aislamientos cl\u00ednicos de acinetobacter baumannii resistentes a imipenem procedentes de hospitales de cochabamba, bolivia<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Identificaci\u00f3n y caracterizaci\u00f3n gen\u00e9tica de la carbapenemasa oxa-58 en aislamientos cl\u00ednicos de acinetobacter baumannii resistentes a imipenem procedentes de hospitales de cochabamba, bolivia <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elena Fernandez Colon <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/05\/2013<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Elena Sevillano Pe\u00f1a<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ramon Cisterna cancer <\/li>\n<li>sara Marti marti (vocal)<\/li>\n<li>Francisco Javier Castillo garcia (vocal)<\/li>\n<li>raul Ortiz de lejarazu leonardo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elena Fernandez Colon La emergencia de a. Baumannii como importante pat\u00f3geno nosocomial ha coincidido con el incremento [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[2193,1418,12909],"tags":[226478,138100,189255,5148,4947,149887],"class_list":["post-114200","post","type-post","status-publish","format-standard","hentry","category-antibioticos","category-bacteriologia","category-pais-vasco-euskal-herriko-unibertsitatea","tag-elena-fernandez-colon","tag-elena-sevillano-pena","tag-francisco-javier-castillo-garcia","tag-ramon-cisterna-cancer","tag-raul-ortiz-de-lejarazu-leonardo","tag-sara-marti-marti"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/114200","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=114200"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/114200\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=114200"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=114200"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=114200"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}