{"id":114534,"date":"2018-03-11T10:42:36","date_gmt":"2018-03-11T10:42:36","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/cultivos-celulares-de-nicotiana-tabacum-l-cv-by-2-como-sistema-modelo-en-el-estudio-de-la-adaptacion-al-estres-salino\/"},"modified":"2018-03-11T10:42:36","modified_gmt":"2018-03-11T10:42:36","slug":"cultivos-celulares-de-nicotiana-tabacum-l-cv-by-2-como-sistema-modelo-en-el-estudio-de-la-adaptacion-al-estres-salino","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/fisiologia-vegetal\/cultivos-celulares-de-nicotiana-tabacum-l-cv-by-2-como-sistema-modelo-en-el-estudio-de-la-adaptacion-al-estres-salino\/","title":{"rendered":"Cultivos celulares de nicotiana tabacum l. cv. by-2 como sistema modelo en el estudio de la adaptaci\u00f3n al estr\u00e9s salino"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jes\u00fas Garcia De La Garma Garcia <\/strong><\/h2>\n<p>Resumen  el objetivo general de esta tesis doctoral es ampliar los conocimientos sobre los mecanismos que permiten la adaptaci\u00f3n de los cultivos celulares de nicotiana tabacum cv. By-2 al estr\u00e9s abi\u00f3tico, concretamente estr\u00e9s salino. Para ello, hemos establecido como objetivos espec\u00edficos los siguientes:  1.\tEstudiar diferentes mecanismos fisiol\u00f3gicos, bioqu\u00edmicos y celulares de adaptaci\u00f3n a salinidad. 2.\tEstudiar el papel que desempe\u00f1an las giberelinas (gas) en el proceso de adaptaci\u00f3n a salinidad. 3.\tEstudiar la implicaci\u00f3n de las prote\u00ednas ricas en arabinogalactanos (agps) y extensinas (exts) en el proceso de adaptaci\u00f3n a salinidad.  el desarrollo de estos objetivos se ha llevado a cabo mediante la utilizaci\u00f3n de t\u00e9cnicas bioqu\u00edmicas, celulares y de biolog\u00eda molecular que nos han permitido obtener una visi\u00f3n m\u00e1s integrada de los mecanismos implicados en la adaptaci\u00f3n de las c\u00e9lulas vegetales al estr\u00e9s salino mediante la regulaci\u00f3n hormonal de los cambios en la estructura y composici\u00f3n de la pared celular. De entre las t\u00e9cnicas empleadas podemos destacar la microscop\u00eda \u00f3ptica, electr\u00f3nica y confocal, la transcript\u00f3mica, prote\u00f3mica e ion\u00f3mica y la inmunolocalizaci\u00f3n mediante el uso de anticuerpos monoclonales.  como conclusiones del trabajo podemos indicar que:  1.\tLas c\u00e9lulas de tabaco by-2 adaptadas a altas concentraciones salinas mostraron importantes modificaciones en la estructura subcelular. En funci\u00f3n de la fase de crecimiento en la que se encontraban, estas c\u00e9lulas presentaron un tama\u00f1o de dos a cuatro veces menor que las c\u00e9lulas crecidas en condiciones control. 2.\tLas c\u00e9lulas de tabaco by-2 adaptadas a altas concentraciones salinas mostraron importantes diferencias ultraestructurales al ser comparadas con las c\u00e9lulas crecidas en condiciones control. Estas diferencias se debieron principalmente a las modificaciones inducidas por la muerte celular observada en las c\u00e9lulas adaptadas. 3.\tEl proceso de adaptaci\u00f3n a altas concentraciones salinas produce importantes modificaciones en el proteoma y transcriptoma de las c\u00e9lulas de tabaco by-2. 4.\tLa adaptaci\u00f3n a altas concentraciones salinas induce la compartimentaci\u00f3n de na+ y el tr\u00e1fico de ves\u00edculas en c\u00e9lulas de tabaco by-2. 5.\tEl metabolismo de la prolina y del aba est\u00e1n implicados en el proceso de adaptaci\u00f3n a altas concentraciones salinas en c\u00e9lulas de tabaco by-2. 6.\tLa producci\u00f3n de ros en la mitocondria puede actuar como modulador en el proceso de adaptaci\u00f3n a altas concentraciones salinas en c\u00e9lulas de tabaco by-2. 7.\tEl sistema de endomembranas, ret\u00edculo endoplasm\u00e1tico y aparato de golgi son modificados en el proceso de adaptaci\u00f3n a salinidad en c\u00e9lulas de tabaco by-2. 8.\tLos cultivos celulares de tabaco by-2 adaptados a salinidad contienen una menor concentraci\u00f3n de giberelinas bioactivas y son sensibles a prohexadiona. 9.\tLa expresi\u00f3n de los genes de s\u00edntesis y degradaci\u00f3n de gas est\u00e1 regulada de forma diferencial en las c\u00e9lulas de tabaco by-2 adaptadas a salinidad. 10.\tLas c\u00e9lulas de tabaco by-2 adaptadas a salinidad acumulan agps en el medio extracelular, posiblemente debido a una mayor tasa de recambio en la membrana plasm\u00e1tica.  11.\tLas c\u00e9lulas de tabaco by-2 adaptadas a salinidad muestran una distribuci\u00f3n diferencial de agps en el citoplasma, membrana plasm\u00e1tica y tonoplasto en comparaci\u00f3n con las c\u00e9lulas control. 12.\tEl reactivo de yariv induce la muerte celular en las c\u00e9lulas de tabaco by-2 control, pero no en las c\u00e9lulas adaptadas a salinidad. 13.\tLas c\u00e9lulas de tabaco by-2 adaptadas a salinidad acumulan extensinas en el medio extracelular, lo que podr\u00eda estar relacionado con una respuesta de defensa frente al estr\u00e9s.                                           summary  the aim of this thesis is to enhance our knowledge about the mechanisms of the cell line nicotiana tabacum by-2 to the stress conditions, in particular to high salinity stress. We have proposed the following specific objectives:  1.\tStudy different physiological, biochemical and cellular mechanisms for adaptation to high salinity. 2.\t Analyze the putative changes in the gas metabolism under the salinity stress conditions and how these changes may affect the plant cells adaptation.  3.\tStudy the involvement of arabinogalactans proteins (agps) and extensins (exts) in the adaptation process to high salinity.  the development of these objectives has been carried out using biochemical, cellular and molecular biology tools. These methods have allowed us to obtain a more integrated view of the mechanisms involved in the adaptation of plant cells to salt stress by regulating hormone changes in the structure and composition of the cell wall. We have been successfully used light, electronic and laser confocal microscopy, transcriptomic, proteomic and ionomic techniques and specific monoclonal antibodies.  we suggest the following conclusions:  1.\tTobacco by-2 cells adapted to high salinity showed significant changes in their subcellular structure. The cellular volume was 2-4 fold smaller in adapted cells.  2.\tTobacco by-2 cells adapted to high salinity showed relevant ultrastructural differences compared with cells grown in control conditions. These differences were mainly due to the modifications induced by cell death observed in adapted cells. 3.\tThe adaptation process to high salinity produces significant changes in the proteome and transcriptome of tobacco by-2 cells. 4.\tAdaptation to high salinity induces the na+ compartimentation and vesicle trafficking in tobacco by-2 cells. 5.\tProline metabolism and aba are involved in the process of adaptation to high salinity in tobacco by-2 cells. 6.\tRos production in mitochondria can act as a modulator in the adaptation process to high salinity in tobacco by-2 cells. 7.\tEndomembrane system, endoplasmic reticulum and golgi apparatus are modified in the process of adaptation to salinity in tobacco by-2 cells. 8.\tCell cultures of by-2 adapted to high salinity contain a lower concentration of bioactive gibberellins and are sensitive to prohexadione compared to control cells. 9.\tThe gas gene expression of synthesis and degradation is differentially regulated in tobacco by-2 cells adapted to high salinity. 10.\tTobacco by-2 cells adapted to high salinity accumulate agps in the extracellular medium, possibly due to higher turnover rate in the plasma membrane. 11.\tTobacco by-2 cells adapted to high salinity exhibit differential agps distribution in the cytoplasm, plasma membrane and tonoplast, compared to control cells. 12.\tYariv reagent induces cell death in tobacco by-2 control cells, but not in cells adapted to high salinity.                                 13.\tTobacco by-2 cells adapted to high salinity accumulate extensins in the extracellular medium, which could be related to a defense response to stress.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Cultivos celulares de nicotiana tabacum l. cv. by-2 como sistema modelo en el estudio de la adaptaci\u00f3n al estr\u00e9s salino<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Cultivos celulares de nicotiana tabacum l. cv. by-2 como sistema modelo en el estudio de la adaptaci\u00f3n al estr\u00e9s salino <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jes\u00fas Garcia De La Garma Garcia <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 18\/07\/2013<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Nieves Fern\u00e1ndez Garc\u00eda<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda del carmen Bolar\u00edn jim\u00e9nez <\/li>\n<li>monica Venegas caleron (vocal)<\/li>\n<li>Jos\u00e9 Antonio  del Rio conesa (vocal)<\/li>\n<li>Jos\u00e9 Luis Casas mart\u00ednez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jes\u00fas Garcia De La Garma Garcia Resumen el objetivo general de esta tesis doctoral es ampliar los [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[479,8235],"tags":[226988,16495,19386,167651,226989,9535],"class_list":["post-114534","post","type-post","status-publish","format-standard","hentry","category-fisiologia-vegetal","category-murcia","tag-jesus-garcia-de-la-garma-garcia","tag-jose-antonio-del-rio-conesa","tag-jose-luis-casas-Martinez","tag-maria-del-carmen-bolarin-jimenez","tag-monica-venegas-caleron","tag-nieves-fernandez-garcia"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/114534","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=114534"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/114534\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=114534"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=114534"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=114534"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}