{"id":114585,"date":"2018-03-11T10:42:42","date_gmt":"2018-03-11T10:42:42","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/efecto-del-17alpha-ethinilestradiol-sobre-el-sistema-inmunitario-y-reproductor-de-la-dorada-sparus-aurata-l-caracterizacion-funcional-del-receptor-de-estrogenos-asociado-a-protea%c2%adna-g\/"},"modified":"2018-03-11T10:42:42","modified_gmt":"2018-03-11T10:42:42","slug":"efecto-del-17alpha-ethinilestradiol-sobre-el-sistema-inmunitario-y-reproductor-de-la-dorada-sparus-aurata-l-caracterizacion-funcional-del-receptor-de-estrogenos-asociado-a-protea%c2%adna-g","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/inmunologia\/efecto-del-17alpha-ethinilestradiol-sobre-el-sistema-inmunitario-y-reproductor-de-la-dorada-sparus-aurata-l-caracterizacion-funcional-del-receptor-de-estrogenos-asociado-a-protea%c2%adna-g\/","title":{"rendered":"Efecto del 17alpha-ethinilestradiol sobre el sistema inmunitario y reproductor de la dorada (sparus aurata l.) caracterizaci\u00f3n funcional del receptor de estr\u00f3genos asociado a prote\u00edna g"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Isabel Cabas S\u00e1nchez <\/strong><\/h2>\n<p>&quot;efecto del 17?-Etinilestradiol sobre el sistema inmunitario y reproductor de la dorada (sparus aurata l.). Caracterizaci\u00f3n funcional del receptor de estr\u00f3genos asociado a prote\u00edna g&quot; d\u00aa. Isabel cabas s\u00e1nchez directores: d\u00aa alfonsa garc\u00eda ayala, d. Victoriano mulero m\u00e9ndez y d. Jos\u00e9 meseguer pe\u00f1alver. objetivos: 1.\tEvaluar la capacidad del ee2 de provocar una respuesta estrog\u00e9nica en vivo en ejemplares macho de dorada 2.\tEvaluar la capacidad del ee2 de alterar la fisiolog\u00eda y la respuesta inmunitaria local de la g\u00f3nada  3.\tEvaluar la habilidad del ee2 de alterar la respuesta inmunitaria sist\u00e9mica, analizando actividades inmunitarias de leucocitos de ri\u00f1\u00f3n cef\u00e1lico, el perfil de expresi\u00f3n de macr\u00f3fagos y la capacidad en vivo de responder a un reto inmunol\u00f3gico.  4.\tRealizar una caracterizaci\u00f3n funcional del receptor de estr\u00f3genos asociado a prote\u00edna g tanto in vitro como in vivo. metodolog\u00eda:  se llevo realiz\u00f3 una experimentaci\u00f3n in vivo e in vitro. Se usaron ejemplares sanos de dorada (sparus aurata l.), Especie hermafrodita prot\u00e1ndrica y de puesta estacional, mantenidos en el centro oceanogr\u00e1fico de murcia (ieo, mazarr\u00f3n, murcia).  \tla experimentaci\u00f3n in vivo se llevo a cabo exponiendo a los ejemplares a ee2 o g1 a diferentes dosis y tiempos. En algunos casos, la exposici\u00f3n se simultane\u00f3 con una inmunizaci\u00f3n programada. A continuaci\u00f3n, se realizaron, entre otros, an\u00e1lisis de supervivencia, de calidad del esperma, histol\u00f3gicos, determinaci\u00f3n de hormonas e igm en suero, de expresi\u00f3n g\u00e9nica y de citometr\u00eda de flujo.  \tla experimentaci\u00f3n in vitro se desarrollo, principalmente, tratando con leucocitos de ri\u00f1\u00f3n cef\u00e1lico con ee2 o g1 (para la activaci\u00f3n espec\u00edfica de la se\u00f1alizaci\u00f3n de gper para su caracterizaci\u00f3n funcional) a diferentes dosis y tiempos y analizando actividades inmunitarias como fagocitosis y explosi\u00f3n respiratoria y el perfil de expresi\u00f3n g\u00e9nica.  conclusiones:  1.\tLa exposici\u00f3n in vivo a ee2 promueve una respuesta estrog\u00e9nica evidente caracterizada por un descenso en el igs, alteraci\u00f3n en los niveles s\u00e9ricos de e2, t y 11kt y una inducci\u00f3n en la expresi\u00f3n hep\u00e1tica de vtg. Estos efectos son m\u00e1s evidentes cuando el ee2 se administra en la dieta que cuando se administra en el agua del ba\u00f1o. Adem\u00e1s, estos efectos dependen, ligeramente, del estado de desarrollo de los ejemplares.   2.\tEl ee2 incrementa la sensibilidad a estr\u00f3genos en g\u00f3nada y en ri\u00f1\u00f3n cef\u00e1lico ya que aumenta la expresi\u00f3n del re?.  3.\tLa exposici\u00f3n in vivo a ee2 interrumpe la espermatog\u00e9nesis e induce en el test\u00edculo la morfolog\u00eda caracter\u00edstica de la etapa de post-puesta. Aunque los t\u00fabulos semin\u00edferos contin\u00faan llenos de espermatozoides se observa una reducci\u00f3n en el volumen y motilidad de los espermatozoides. Adem\u00e1s, el ee2  genera un proceso pro-inflamatorio en la g\u00f3nada, promoviendo una infiltraci\u00f3n masiva de leucocitos y un incremento en la expresi\u00f3n de citoquinas y mol\u00e9culas implicadas en el reconocimiento y presentaci\u00f3n de ant\u00edgenos.   4.\tEl ee2 disminuye la capacidad de los ejemplares de responder a un est\u00edmulo inmunol\u00f3gico  in vivo ya que inhibe la producci\u00f3n de citoquinas pro-inflamatorias tras una inmunizaci\u00f3n aunque no se comporta como inmunosupresor. Adem\u00e1s, el ee2 inhibe in vitro las actividades inmunitarias de leucocitos de ri\u00f1\u00f3n cef\u00e1lico y dirige hacia un fenotipo anti-inflamatorio en macr\u00f3fagos.  5.\tEl gper se expresa en tejidos reproductores e inmunitarios de dorada y su expresi\u00f3n no es modulada por su activaci\u00f3n.  6.\tLos granulocitos acid\u00f3filos son las c\u00e9lulas del ri\u00f1\u00f3n cef\u00e1lico que tienen un mayor nivel de expresi\u00f3n de gper.  \u00e9stos expresan un gper funcional cuya activaci\u00f3n in vitro dirige, de manera general, hacia un fenotipo anti-inflamatorio. Dichos efectos son regulados, en parte, por la activaci\u00f3n de la ruta de se\u00f1alizaci\u00f3n ampc\/pka\/creb.   7.\tLa activaci\u00f3n de gper no promueve una respuesta estrog\u00e9nica aunque provoca, en general, un efecto anti-inflamatorio y modula ligeramente la respuesta inmunitaria adaptativa.    &quot;effect of 17?-Ethinylestradiol on immune and reproductive system of the gilthead seabream (sparus aurata l.). Functional characterization of the g protein-coupled estrogen receptor&quot; d\u00aa. Isabel cabas s\u00e1nchez directores: d\u00aa alfonsa garc\u00eda ayala, d. Victoriano mulero m\u00e9ndez y d. Jos\u00e9 meseguer pe\u00f1alver. objetives: 1.\tTo evaluate the ability of ee2 to provoke an estrogenic response in vivo in male gilthead seabream.   2.\tTo evaluate the ability of ee2 to alter the physiology and the local immune response of the gonad.  3.\tTo evaluate the ability of ee2 to alter the systemic immune response, analysing immune activities of head kidney leukocytes, the expression profile of macrophages and the in vivo capabilities to respond to an immune challenge.  4.\tTo perform a functional characterization of the g protein-coupled estrogen receptor, gper, both in vitro and in vivo. methodology:  in vivo and in vitro experimentation was carried out. For this, healthy specimens of gilthead seabream (sparus aurata l.) Were bred and kept at the centro oceanogr\u00e1fico de murcia (ieo, mazarr\u00f3n, murcia).  in vivo experiments were carried out by exposing the specimens to ee2 or g1 at different doses and times. In some cases, the exposure was simultaneous to a scheduled immunization. Afterwards, analysis of survival, sperm quality, histological, determination of hormones and igm in serum, gene expression and flow cytometry were performed. in general, for the in vitro experiments, leukocytes from head kidney (bone marrow equivalent) were treated with several doses of ee2 and g1 (to specifically activate gper signalling) at different time points, and analyzed immune activities such as phagocytosis and respiratory burst and gene expression profile. conclusions:  1.\tExposure to ee2 in vivo promotes an evident estrogenic response characterized by decreased gsi, altered serum levels of e2, t and 11kt, and induced hepatic expression of vtg. These effects are more evident when ee2 is administered in the diet than when is administered in bath water. In addition, its effects slightly depend on the development stage of the specimens.   2.\tEe2 increases the sensitivity to estrogens in the gonad and the head kidney by increasing the expression of the gene coding for er?.   3.\tExposure to ee2 in vivo disrupts spermatogenesis and induces a characteristic morphology of the post-spawning in the testis. However, the seminiferous tubules were filled with sperm, causing a reduction in the volume and sperm motility. Moreover, ee2 also generates a pro-inflammatory process in the gonad, promoting a massive infiltration of leukocytes and an increased expression of the genes encoding cytokines and molecules involved in antigen recognition and presentation.  4.\tEe2 decreases the ability of specimens to respond to an immune stimulus in vivo by inhibiting the production of pro-inflammatory cytokines after immunization but does not behave as an immunosuppressor. Moreover, ee2 inhibits in vitro the immune activities of head kidney leukocytes and direct primary macrophages towards an anti-inflammatory phenotype.  5.\tGper is expressed in reproductive and immune tissues in gilthead seabream and its expression is not modulated by its activation.   6.\tAcidophilic granulocytes are the head kidney cells with a higher level of expression of gper. They express a functional gper whose activation in vitro leads, in general, to an anti-inflammatory phenotype. These effects are regulated, in part, by activation of the camp\/pka\/creb signalling pathway.   7.\tGper activation in vivo does not promote an estrogenic response, although in general, provokes an anti-inflammatory effect and slightly modulates the adaptive immune response.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Efecto del 17alpha-ethinilestradiol sobre el sistema inmunitario y reproductor de la dorada (sparus aurata l.) caracterizaci\u00f3n funcional del receptor de estr\u00f3genos asociado a prote\u00edna g<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Efecto del 17alpha-ethinilestradiol sobre el sistema inmunitario y reproductor de la dorada (sparus aurata l.) caracterizaci\u00f3n funcional del receptor de estr\u00f3genos asociado a prote\u00edna g <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Isabel Cabas S\u00e1nchez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/07\/2013<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Alfonsa Garcia Ayala<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Antonio Bernabe salazar <\/li>\n<li>nerea Roher armentia (vocal)<\/li>\n<li>Mar\u00eda  Luisa Cayuela fuentes (vocal)<\/li>\n<li>Jes\u00fas Lamas fern\u00e1ndez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Isabel Cabas S\u00e1nchez &quot;efecto del 17?-Etinilestradiol sobre el sistema inmunitario y reproductor de la dorada (sparus aurata [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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