{"id":114813,"date":"2018-03-11T10:42:56","date_gmt":"2018-03-11T10:42:56","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/optimization-of-cell-microencapsulation-in-terms-of-biosafety-biomimesis-and-applicability-in-therapeutic-targets-of-central-nervous-system\/"},"modified":"2018-03-11T10:42:56","modified_gmt":"2018-03-11T10:42:56","slug":"optimization-of-cell-microencapsulation-in-terms-of-biosafety-biomimesis-and-applicability-in-therapeutic-targets-of-central-nervous-system","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/evaluacion-de-farmacos\/optimization-of-cell-microencapsulation-in-terms-of-biosafety-biomimesis-and-applicability-in-therapeutic-targets-of-central-nervous-system\/","title":{"rendered":"Optimization of cell microencapsulation in terms of biosafety, biomimesis and applicability in therapeutic targets of central nervous system"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Edorta Santos Vizcaino <\/strong><\/h2>\n<p>On the basis of the results obtained in the experimental studies of thisdissertation, the following conclusions were derived:1. The incorporation of the psfgnestgl into cells resulted in an accurate toolfor holding a tight control over microencapsulated cells. More importantly,this method provided meaningful information about the functionality ofthe enclosed cells at desired time points and in a non-invasive way,avoiding the necessity to retrieve the implant. This would help todetermine the precise location of the therapeutic biosystems in vivo and tomonitor encapsulated cells in real-time.2. Encapsulated cells transduced with the tgl triple-fusion reporter genesuccessfully responded to gcv administration suffering apoptosis andleading to implant inactivation in vivo.3. The present work provided meaningful insight regarding gcv-mediatedinactivation process in encapsulated cells bearing tgl triple-fusionreporter gene through its fused hsv-tk suicide gene.4. The inclusion of inactivation gene vectors in the genome of encapsulatedcells may represent a valuable strategy to overcome some of the biosafetyconcerns in the field of cell microencapsulation. In this work, tgl triplefusionreporter gene demonstrated satisfactory results in regulating therelease of therapeutic factors, and it is proposed as a starting point towork in this direction.5. The present study covered a comprehensive technological development toachieve an 80% size reduction of classic apa microcapsules and obtainhighly monodisperse 100 \u00c2\u00bfm particles while preserving cell viability andfunction. In addition, this work reinforced the suitableness of apa capsuleseven at this particular size.6. The final stage of this thesis entailed the optimization of intraocularadministration of 100 \u00c2\u00bfm apas and proved the correct adaptation of theimplant to the vitreous humor environment. This opens the doors tofurther investigations, where the effectiveness of this technology might betested in a retinopathy model.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Optimization of cell microencapsulation in terms of biosafety, biomimesis and applicability in therapeutic targets of central nervous system<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Optimization of cell microencapsulation in terms of biosafety, biomimesis and applicability in therapeutic targets of central nervous system <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Edorta Santos Vizcaino <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 31\/10\/2013<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Gorka Orive Arroyo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: alfonso Jes\u00fas Dominguez gil hurle <\/li>\n<li>josep Samitier &#8212; (vocal)<\/li>\n<li>M\u00aaangeles Solinis aspiazu (vocal)<\/li>\n<li>james Kirkpatrick chales (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Edorta Santos Vizcaino On the basis of the results obtained in the experimental studies of thisdissertation, the [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[2445,12909,3304],"tags":[3222,227424,76788,227426,227425,177809],"class_list":["post-114813","post","type-post","status-publish","format-standard","hentry","category-evaluacion-de-farmacos","category-pais-vasco-euskal-herriko-unibertsitatea","category-preparacion-de-farmacos","tag-alfonso-jesus-dominguez-gil-hurle","tag-edorta-santos-vizcaino","tag-gorka-orive-arroyo","tag-james-kirkpatrick-chales","tag-josep-samitier","tag-maangeles-solinis-aspiazu"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/114813","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=114813"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/114813\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=114813"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=114813"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=114813"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}