{"id":114892,"date":"2018-03-11T10:43:10","date_gmt":"2018-03-11T10:43:10","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-del-efecto-del-extracto-de-humulus-lupulus-l-sobre-un-modelo-de-osteoporosis-en-ratas-sprague-dawley\/"},"modified":"2018-03-11T10:43:10","modified_gmt":"2018-03-11T10:43:10","slug":"estudio-del-efecto-del-extracto-de-humulus-lupulus-l-sobre-un-modelo-de-osteoporosis-en-ratas-sprague-dawley","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/patologia-experimental\/estudio-del-efecto-del-extracto-de-humulus-lupulus-l-sobre-un-modelo-de-osteoporosis-en-ratas-sprague-dawley\/","title":{"rendered":"Estudio del efecto del extracto de humulus lupulus l. sobre un modelo de osteoporosis en ratas sprague-dawley."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ester Mostazo Mendoza <\/strong><\/h2>\n<p>Estudio del efecto del extracto de humulus lupulus l. Sobre un modelo de osteoporosis en ratas sprague-dawley. resumen. la osteoporosis es una patolog\u00eda caracterizada por la p\u00e9rdida progresiva de masa \u00f3sea debido a un desequilibrio en el remodelado \u00f3seo que provoca que las trab\u00e9culas sean m\u00e1s finas, est\u00e9n menos conectadas y se originen microfracturas, y a largo o corto plazo, puedan provocar una fractura del hueso (gonz\u00e1lez, 2004).  los flavonoides son compuestos fen\u00f3licos muy abundantes en la naturaleza con capacidad antioxidante. Algunos de ellos poseen una estructura qu\u00edmica muy similar a los estr\u00f3genos, y son capaces de unirse a sus receptores, activ\u00e1ndolos, por lo que pueden ejercer una funci\u00f3n an\u00e1loga  (milligan, 2000; schaefer, 2003). Por este y otros motivos, se est\u00e1 considerando su inclusi\u00f3n como suplemento alimentario.  el objetivo de nuestro estudio ha sido analizar el efecto de los flavonoides contenidos en el extracto de humulus lupulus l. Sobre un modelo de osteoporosis secundaria a ovariectom\u00eda en ratas sprague-dawley a trav\u00e9s de diferentes estudios: del peso de los animales, bioqu\u00edmico general y de la fosfatasa alcalina \u00f3sea, estudio de la distribuci\u00f3n de la masa \u00f3sea y cuantificaci\u00f3n de la densidad mineral mediante microtomograf\u00eda computerizada, estudio anatomo-patol\u00f3gico del grosor de las trab\u00e9culas \u00f3seas y del espesor del epitelio luminal del endometrio uterino a trav\u00e9s del microscopio \u00f3ptico, y por \u00faltimo, el estudio ion\u00f3mico de los componentes qu\u00edmicos del hueso mediante espectrofotometr\u00eda. para ello, utilizamos 40 ratas sprague-dawley distribuidas en cuatro grupos (n=10): i: control; ii: control ovariectom\u00eda; iii: ovariectom\u00eda + ehl d1 (0,032 g\/kg) y iv: ovariectom\u00eda + ehl d2 (0,32 g\/kg). La ovariectom\u00eda realizada a los grupos ii, iii y iv fue bilateral y tras cuatro meses de tratamiento, los animales fueron sacrificados para extraer los huesos f\u00e9mur y tibia y los \u00fateros para su posterior an\u00e1lisis. Durante el experimento se pes\u00f3 a los animales peri\u00f3dicamente y se les extrajo sangre para realizar el estudio bioqu\u00edmico en tres tiempos: antes de ser tratadas, dos meses despu\u00e9s de la intervenci\u00f3n y al sacrificio. al final del estudio los animales tratados presentaron un retraso del aumento de peso comparado con el grupo intervenido sin tratar. En el estudio bioqu\u00edmico, los niveles s\u00e9ricos de calcio y f\u00f3sforo se mantuvieron estables en todos los grupos experimentales y los niveles de fosfatasa alcalina total y \u00f3sea descendieron en todos los grupos. sin embargo, nuestros resultados m\u00e1s relevantes fueron observados a nivel \u00f3seo. As\u00ed, tanto el grosor como el volumen de las trab\u00e9culas de los huesos de los animales tratados con ehl fueron mayores que en los animales ovariectomizados no tratados (constatado mediante histomorfometr\u00eda y microtomograf\u00eda computerizada). Estos animales, adem\u00e1s, sufrieron menor p\u00e9rdida de los minerales que conforman la matriz \u00f3sea que los controles operados, como confirm\u00f3 el estudio ion\u00f3mico. Al estudiar el epitelio endometrial uterino, observamos que el de los animales tratados con ehl mostraba proliferaci\u00f3n y presencia de gl\u00e1ndulas similar a la normalidad (control no operado), mientras que en los animales operados no tratados, dicho epitelio presentaba signos claros de atrofia. por todo ello, concluimos que la ingesta de ehl ha sido efectiva en el tratamiento de la osteoporosis provocada por deprivaci\u00f3n hormonal en nuestro estudio, siendo necesario profundizar m\u00e1s en el conocimiento de este agente en futuros estudios que permitan clarificar su mecanismo de acci\u00f3n y seguir avanzando en su posible uso en cl\u00ednica humana.    study of humulus lupulus l effects on an osteoporosis model in sprague-dawley rats. abstract. osteoporosis is a disease characterized by progressive mass bone loss due to an imbalance on bone remodeling that causes the trabeculae to be thinner, less connected and microfractures to be generated which, in short or longer term, will inflict a bone fracture (gonz\u00e1lez, 2004). flavonoids are phenolic compounds very abundant in nature with antioxidant capacity. The inclusion of flavonoids in the intake as a food supplement is being considered. Some flavonoids have a chemical structure very similar to estrogens, and they are able to bind to their receptors, activating them, so they can play an analogous role (milligan, 2000; schaefer, 2003). at the end of our study, treated animals showed delayed weight gain compared to untreated intervention group. In the biochemical study, serum levels of calcium and phosphorus remained stable in all experimental groups and total alkaline phosphatase levels and bone decreased in all groups. the purpose of our study was to analyze the effect of the flavonoid content in the humulus lupulus l. (Ehl) extract on a model of osteoporosis secondary to ovariectomy in sprague-dawley rats through different studies: weight of the animals, general biochemistry and bone alkaline phosphatase studies, study of the bone mass distribution and quantification of the mineral density via computerized microtomography, anatomopathologic study of the thickness of the bone trabeculae and the luminal epithelium thickness in the uterine endometrium through the optic microscope and, finally, the ion measurement study of the chemical compounds of the bone via spectrophotometry. to do this, we used 40 sprague-dawley rats, distributed in four groups (n=10): i: control; ii: ovariectomy control; iii: ovariectomy + ehl d1 (0,032 g\/kg) y iv: ovariectomy + ehl d2 (0,32 g\/kg). The ovariectomy done to groups ii, iii y iv was bilateral and after four months being treated they were sacrificed to extract each animal&apos;s femur, tibia and uterus for subsequent analysis. During the experiment the animals were weighed periodically and blood was extracted for biochemical analysis on three occasions: before treatment, two months after the intervention and at the time of sacrifice.  the most relevant results in our study were observed at the bone level. Therefore, both trabeculae thickness and volume in the bones of the animals treated with ehl were higher than in the ovariectomized untreated animals (determined via histomorphometry and microtomography). These animals, besides, suffered from a lower loss of the minerals compounding the bone matrix than the surgically operated control ones, confirmed by the ion measurement study. By studying the endometrial uterine epithelium, we realized that the one in the animals treated with ehl showed proliferation and presence of glands similar to the normal one (non-operated control), while in untreated operated animals, this epithelium showed clear signs of atrophy. therefore, we conclude that the intake of ehl was effective in the treatment of osteoporosis caused by hormonal deprivation in our study, being necessary to go deeper into the knowledge of this agent in future studies which clarify its mechanism of action and further progress in its use in clinical practice.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio del efecto del extracto de humulus lupulus l. sobre un modelo de osteoporosis en ratas sprague-dawley.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio del efecto del extracto de humulus lupulus l. sobre un modelo de osteoporosis en ratas sprague-dawley. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ester Mostazo Mendoza <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 22\/11\/2013<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Vicente Vicente Ortega<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 joaqu\u00edn Cer\u00f3n madrigal <\/li>\n<li>Fernando Pascual carpe (vocal)<\/li>\n<li>Emilio Jos\u00e9 Salmer\u00f3n mart\u00ednez (vocal)<\/li>\n<li>gerardo G\u00f3mez moreno (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ester Mostazo Mendoza Estudio del efecto del extracto de humulus lupulus l. Sobre un modelo de osteoporosis [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[34275,8235,1323],"tags":[175421,227541,156706,58829,65957,8486],"class_list":["post-114892","post","type-post","status-publish","format-standard","hentry","category-antioxidantes-en-los-alimentos","category-murcia","category-patologia-experimental","tag-emilio-jose-salmeron-Martinez","tag-ester-mostazo-mendoza","tag-fernando-pascual-carpe","tag-gerardo-gomez-moreno","tag-jose-joaquin-ceron-madrigal","tag-vicente-vicente-ortega"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/114892","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=114892"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/114892\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=114892"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=114892"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=114892"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}