{"id":11524,"date":"2018-03-09T08:56:56","date_gmt":"2018-03-09T08:56:56","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/expresion-y-caracterizacion-de-la-protea%c2%adna-gp5-del-virus-del-sa%c2%adndrome-reproductor-y-respiratorio-porcino\/"},"modified":"2018-03-09T08:56:56","modified_gmt":"2018-03-09T08:56:56","slug":"expresion-y-caracterizacion-de-la-protea%c2%adna-gp5-del-virus-del-sa%c2%adndrome-reproductor-y-respiratorio-porcino","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/expresion-y-caracterizacion-de-la-protea%c2%adna-gp5-del-virus-del-sa%c2%adndrome-reproductor-y-respiratorio-porcino\/","title":{"rendered":"Expresi\u00f3n y caracterizaci\u00f3n de la prote\u00edna gp5 del virus del s\u00edndrome reproductor y respiratorio porcino."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Aurora Fern\u00e1ndez Garc\u00eda <\/strong><\/h2>\n<p>La tesis doctoral  se ha dirigido a la expresi\u00f3n de la prote\u00edna gp5 del virus del s\u00edndrome reproductor y respiratorio porcino (vsrrp) en diferentes sistemas y su caracterizaci\u00f3n. La prote\u00edna gp5 est\u00e1 codificada por la orf5 del genoma del vsrrp, es la glicoprote\u00edna mayoritaria de la envoltura del viri\u00f3n y posee ep\u00edtopos antig\u00e9nicos implicados en la inducci\u00f3n de anticuerpos que neuralizan la infecci\u00f3n por el vsrrp, por lo que es de gran inter\u00e9s para el desarrollo de posibles vacunas frente a la enfermedad.  el primer objetivo de esta tesis se centr\u00f3 en la producci\u00f3n de la prote\u00edna gp5 en un sistema eucariota que asegurar las modificaciones postraduccionales de la misma. Para ello se utiliz\u00f3 como vector de expresi\u00f3n un virus de la enfermedad de aujeszky (adv) recombinante de fenotipo tk negativo y ge negativo, de similares caracter\u00edsticas a las vacunas atenuadas utilizadas en la actualidad en las campa\u00f1as de control de la enfermedad. As\u00ed se insert\u00f3 mediante recombinaci\u00f3n hom\u00f3loga la orf5 de una cepa espa\u00f1ola del vsrrp bajo el control del promotor de la prote\u00edna temprana gg de adv, en el lugar del gen que codifica la ge del adv, y se obtuvo el virus prv7. La expresi\u00f3n de la prote\u00edna se pudo determinar por inmunoflurescencia indirecta en c\u00e9lulas pk 15 infectadas con el virus. Los resultados obtenidos sugieren que el virus prv7 podr\u00eda ser utilizado como un posible producto vacunal bivalente frente a las dos enfermedades.  aun as\u00ed la expresi\u00f3n de la prote\u00edna gp5 obtenida en sistemas eucariotas es poco eficiente y esto podr\u00eda relacionarse, al menos en parte, con la capacidad de inducir apoptosis de la citada prote\u00edna dlevsrrp. As\u00ed se abord\u00f3 el siguiente objetivo de la tesis doctoral con el fin de estudiar las regiones de la prote\u00edna gp5 implicadas en la inducci\u00f3n de apoptosis y obtener mutantes que carecieran de las mismas y no fueran letales para las c\u00e9lulas para mejorar la eficiencia de la expresi\u00f3n. Par<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Expresi\u00f3n y caracterizaci\u00f3n de la prote\u00edna gp5 del virus del s\u00edndrome reproductor y respiratorio porcino.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Expresi\u00f3n y caracterizaci\u00f3n de la prote\u00edna gp5 del virus del s\u00edndrome reproductor y respiratorio porcino. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Aurora Fern\u00e1ndez Garc\u00eda <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 22\/06\/2001<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Castro Arganda Jos\u00e9 M.<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: lucas Dom\u00ednguez rodr\u00edguez <\/li>\n<li>enrique Espu\u00f1a mas\u00f3 (vocal)<\/li>\n<li>Francisco Sobrino castello (vocal)<\/li>\n<li>ignacio Casal \u00e1vlarez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Aurora Fern\u00e1ndez Garc\u00eda La tesis doctoral se ha dirigido a la expresi\u00f3n de la prote\u00edna gp5 del [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35,280,5590],"tags":[37872,4492,37873,14395,37874,2658],"class_list":["post-11524","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","category-virologia","category-virologia-animal","tag-aurora-fernandez-garcia","tag-castro-arganda-jose-m","tag-enrique-espuna-maso","tag-francisco-sobrino-castello","tag-ignacio-casal-avlarez","tag-lucas-dominguez-rodriguez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/11524","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=11524"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/11524\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=11524"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=11524"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=11524"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}