{"id":115849,"date":"2018-03-11T10:44:36","date_gmt":"2018-03-11T10:44:36","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-la-biocompatibilidad-celular-de-distintos-materiales-utilizados-en-los-recubrimientos-pulpares\/"},"modified":"2018-03-11T10:44:36","modified_gmt":"2018-03-11T10:44:36","slug":"estudio-de-la-biocompatibilidad-celular-de-distintos-materiales-utilizados-en-los-recubrimientos-pulpares","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/estomatologia-y-ortodoncia\/estudio-de-la-biocompatibilidad-celular-de-distintos-materiales-utilizados-en-los-recubrimientos-pulpares\/","title":{"rendered":"Estudio de la biocompatibilidad celular de distintos materiales utilizados en los recubrimientos pulpares"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Diego Saura Mi\u00f1ano <\/strong><\/h2>\n<p>Estudio de biocompatibilidad celular de distintos materiales utilizados en los recubrimientos pulpares   la protecci\u00f3n pulpar indirecta, o recubrimiento pulpar indirecto, es el tratamiento de caries profundas, mediante el cual evitamos a prop\u00f3sito la entrada en la c\u00e1mara pulpar, para colocar una base cavitaria y favorecer los mecanismos biol\u00f3gicos de curaci\u00f3n.  est\u00e1 indicado en casos de caries profundas asintom\u00e1ticas o con pulpitis reversible, sin signos ni s\u00edntomas de inflamaci\u00f3n irreversible, dolor o infecci\u00f3n.  para este procedimiento utilizamos diversos materiales, como son los ion\u00f3meros de vidrio, el hidr\u00f3xido de calcio o la hidroxiapatita. Estos materiales deben cumplir con los requisitos cl\u00ednicos necesarios para poder ser ubicados en el fondo de la cavidad y estimular la curaci\u00f3n pulpar y la formaci\u00f3n de un puente dentinario entre la restauraci\u00f3n y la pulpa.   adem\u00e1s deben ser biocompatibles, cuando se eval\u00faan mediante tests directos e indirectos, sobre animales vivos y cultivos celulares respectivamente.   objetivos  el objetivo de este trabajo ha sido evaluar y comparar varios materiales de uso cl\u00ednico que podr\u00edan utilizarse en protecci\u00f3n pulpar indirecta en dentici\u00f3n temporal, mediante un ensayo de biocompatibilidad, as\u00ed como analizar los cambios morfol\u00f3gicos que dichos materiales causan sobre las c\u00e9lulas, y de este modo, atendiendo a su propiedad de biocompatibilidad, determinar cu\u00e1l podr\u00eda ser el m\u00e1s indicado.  material y m\u00e9todo  se realiz\u00f3 un ensayo de toxicidad mediante cultivos celulares de la l\u00ednea celular l929. Las c\u00e9lulas fueron expuestas durante 24 horas a diluciones seriadas de extractos de cuatro cementos de uso en protecci\u00f3n pulpar indirecta: ion\u00f3meros de vidrio foto y autopolimerizable (vitrebond plus y ketac bond), hidroxiapatita (fluoroseal) e hidr\u00f3xido de calcio fotopolimerizable (calcimol lc).  se evalu\u00f3 el efecto causado de forma cualitativamente mediante microscopio \u00f3ptico, y cuantitativamente mediante un an\u00e1lisis estad\u00edstico.  resultados  el an\u00e1lisis de los cultivos celulares mostr\u00f3 que a m\u00e1xima concentraci\u00f3n todos los materiales eran t\u00f3xicos, pero que vitrebond plus es el menos biocompatible de los materiales para el resto de las concentraciones, y ketac bond el m\u00e1s biocompatible (p&lt;0,05), siendo los otros dos materiales bastante biocompatibles, y similares entre s\u00ed.  tambi\u00e9n se hall\u00f3 que la optimizaci\u00f3n del ph de los materiales puede influir mucho sobre su biocompatibilidad.   biocompatibility essay on cell cultures of various pulp capping materials   indirect pulp capping is the treatment of deep carious lesions that involves the removal of all the infected dentin, as the affected dentin is left in place, in order to avoid pulp exposure. A pulp liner is placed in the floor of the cavity to stimulate the natural healing and repair procedures.  it is indicated in deep carious lesions without symptoms of degeneration, pain or infection.  several materials can be used for this procedure as glass ionomers, calcium hydroxide or hidroxyapatite. These materials have to accomplish the clinical needs to be placed in the cavity not to interrupt the pulp healing procedures, and stimulate the dentin bridge formation.  besides, the materials have to be biocompatible, what is evaluated by direct and indirect tests, on animals or cell cultures.  objectives  the aim of this study was to evaluate and compare different pulp capping materials for primary teeth, by a cytotoxicity essay, and analyse morphologic changes when observing with optical microscopy, and so determine which one is the least toxic, and so on, the most accurate for indirect pulp treatment.  material and methods  a cytotoxicity essay with l929 cell cultures was made. Cells were exposed for 24 hours to serial dilutions of four cavity liners: auto and light curing glass ionomers (ketac bond and vitrebond plus), hydroxiapatite (fluoroseal) and light curing calcium hydroxide (calcimol lc).  the effect of the materials on the cells was evaluated with optical microscope, and analysed statistically.  results  at the highest concentration, all the materials were toxic, being vitrebond plus the less biocompatible at all the concentrations, and ketac bond the most biocompatible (p&lt;0,05). Fluoroseal and calcimol lc showed similar results.  it was also found, that the optimization of the ph of the materials might have influence in their biocompatibility.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio de la biocompatibilidad celular de distintos materiales utilizados en los recubrimientos pulpares<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de la biocompatibilidad celular de distintos materiales utilizados en los recubrimientos pulpares <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Diego Saura Mi\u00f1ano <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 20\/06\/2014<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Olga Cortes Lillo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  ramon Boj quesada <\/li>\n<li>montserrat Catala pizarro (vocal)<\/li>\n<li>paola Beltri orta (vocal)<\/li>\n<li>Mar\u00eda  antonia Alcaina lorente (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Diego Saura Mi\u00f1ano Estudio de biocompatibilidad celular de distintos materiales utilizados en los recubrimientos pulpares la protecci\u00f3n 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