{"id":116223,"date":"2018-03-11T10:45:12","date_gmt":"2018-03-11T10:45:12","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/genoarquitectura-comparada-del-mesencefalo-alar-en-el-raton-y-el-pollo\/"},"modified":"2018-03-11T10:45:12","modified_gmt":"2018-03-11T10:45:12","slug":"genoarquitectura-comparada-del-mesencefalo-alar-en-el-raton-y-el-pollo","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/desarrollo-animal\/genoarquitectura-comparada-del-mesencefalo-alar-en-el-raton-y-el-pollo\/","title":{"rendered":"Genoarquitectura comparada del mesenc\u00e9falo alar en el rat\u00f3n y el pollo."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Abdelmalik Ayad <\/strong><\/h2>\n<p>Resumen objetivo general: caracterizar genoarquitectonicamente de modo comparado la placa alar mesencef\u00e1lica del rat\u00f3n y el pollo durante el desarrollo y la vida postnatal temprana. materiales y m\u00e9todos: se utilizaron ratones de la cepa swiss albino. Se analizaron embriones de e10.5, e11.5, e12.5, e14.5, e16.5 y e18.5, as\u00ed como algunos estadios postnatales y el adulto (p0, p10-14, p25 y p56). En el estudio de aves se utilizaron embriones de pollo (hh30-hh45) y algunos estadios postnatales (p1, p2, p6). Para llevar a cabo el an\u00e1lisis pretendido se realizaron hibridaciones in situ con m\u00faltiples sondas de rnam en embriones in toto, o en secciones de vibratomo y de criostato. El material procesado fue escaneado utilizando el software image scope. Finalmente los datos fueron seleccionados para su interpretaci\u00f3n y an\u00e1lisis. resultados: como resultado del an\u00e1lisis genoartiquect\u00f3nico se caracteriz\u00f3 molecularmente los l\u00edmites rostral y caudal del mesenc\u00e9falo de rat\u00f3n y pollo durante el desarrollo y algunos estadios postnatales. Adem\u00e1s de los ya conocidos marcadores pax6, tcf7l2 y fgf8, otros factores de transcripci\u00f3n tales como lim1, lhx2, foxp1, tcfap2b, dbx1, esrrb y foxb1 resultaron \u00fatiles para identificar estos l\u00edmites. Adem\u00e1s estos datos genoarquitect\u00f3nicos han contribuido a clarificar los l\u00edmites entre los dominios alares anteroposteriores y los subdominios dorsoventrales de m1 y m2 (mesomeros 1 and 2). Se ha llevado a cabo un detallado estudio genoarquitect\u00f3nico comparado de los col\u00edculos superior e inferior (torus semicircular) del pollo y el rat\u00f3n, lo cual ha permitido confirmar y en su caso postular homolog\u00edas de sus componentes estructurales (capas, n\u00facleos, poblaciones neuronales) entre ambas especies. Otras regiones comprendidas en este estudio son el gris tectal y el \u00e1rea pre\u00edstmica. conclusiones: los datos obtenidos en este estudio permiten disponer de marcadores adicionales para localizar con precisi\u00f3n los l\u00edmites del mesenc\u00e9falo, as\u00ed como tambi\u00e9n identificar las regiones y subregiones alares que componen los mes\u00f3meros 1 y 2. Los abundantes datos moleculares obtenidos contribuir\u00e1n a futuros estudios de los dominios alares mesencef\u00e1licos, cuya finalidad sea comprender los fen\u00f3menos causales de su proceso de laminaci\u00f3n o especificaci\u00f3n celular diferencial. Finalmente, se aporta la definici\u00f3n de un nuevo territorio histogen\u00e9tico radial intercalado entre los col\u00edculos superior e inferior, el \u00e1rea intercollicular. Por otra parte el detallado an\u00e1lisis genoarquitect\u00f3nico comparado del col\u00edculo inferior\/torus semicircular entre pollo y rat\u00f3n ha permitido postular posibles homolog\u00edas entre ambas especies.   summary  general aim: characterize comparatively the genoarchitectonic structure of the mesencephalic alar plate of the mouse and chick during development and early postnatal life. methods: the study used mice wild albino swiss strain. We analyzed embryos at e10.5, e11.5, e12.5, e14.5, e16.5 and e18.5; as well as selected postnatal stages and the adult (p0, p10-14, p25 y p56). To carry out the intended analysis multiple in situ hybridizations with marn probes were performed on whole embryos, in vibratome sections and cryostat sections. In selected cases an immunohistochemical reaction served as counterstaining of the hybridization. The processed material was scanned using image scope software. Finally, specific data were selected for further interpretation and analysis. results: using our genoarchitectonic approach we characterized molecularly the rostral and caudal midbrain limits during all stages of development and some postnatal stages. Besides the known relevant markers pax6, tcf7l2 and fgf8, other patterns of transcription factors such as lim1, lhx2, foxp1, tcfap2b, dbx1, foxb1 and esrrb were useful to identify these limits. Furthermore, these genoarchitectonic data helped to clarify the boundaries between the various anteroposterior alar domains and their respective dorsoventral subdomains across m1 and m2 (mesomeres 1 and 2). Finally, the analysis allowed obtaining data on the selective expression of genes in the different strata that make up the layers of the superior and inferior colliculi. Other studied regions were the tectal gray, the intercollicular area and the preisthmic area. conclusions: the data obtained in this study identified additional markers that accurately locate the boundaries of the midbrain, and served to identify genoarchitectonically distinct regions that make up the alar plate of mesomere 1. The novel intercollicular area was identified as a full radial histogenetic zone. Finally, abundant molecular data were generated that will contribute to future causal developmental studies of the superior and inferior colliculi, as regards the process of layering or cell specification. Moreover, detailed postulates of homology were proposed between the structural components of the mammalian inferior colliculus and the avian torus semicircularis.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Genoarquitectura comparada del mesenc\u00e9falo alar en el rat\u00f3n y el pollo.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Genoarquitectura comparada del mesenc\u00e9falo alar en el rat\u00f3n y el pollo. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Abdelmalik Ayad <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 28\/07\/2014<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Luis Puelles Lopez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: salvador Mart\u00ednez p\u00e9rez <\/li>\n<li>carmen D\u00edaz delgado (vocal)<\/li>\n<li>matias Hidalgo s\u00e1nchez (vocal)<\/li>\n<li>eduardo De puelles Martinez de la torre (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Abdelmalik Ayad Resumen objetivo general: caracterizar genoarquitectonicamente de modo comparado la placa alar mesencef\u00e1lica del rat\u00f3n y [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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