{"id":116844,"date":"2018-03-11T10:46:08","date_gmt":"2018-03-11T10:46:08","guid":{"rendered":""},"modified":"2018-03-11T10:46:08","modified_gmt":"2018-03-11T10:46:08","slug":"evaluation-and-optimization-of-immunomagnetic-methods-for-the-purification-and-selection-of-cmv-specific-cytotoxic-t-lymphocytes-from-g-csf-primed-peripheral-blood-mononuclear-cells-for-adoptive-immun","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/inmunologia\/evaluation-and-optimization-of-immunomagnetic-methods-for-the-purification-and-selection-of-cmv-specific-cytotoxic-t-lymphocytes-from-g-csf-primed-peripheral-blood-mononuclear-cells-for-adoptive-immun\/","title":{"rendered":"Evaluation and optimization of immunomagnetic methods for the purification and selection of cmv-specific cytotoxic t lymphocytes from g-csf primed peripheral blood mononuclear cells for adoptive immunotherapy"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Lorea Beloki Bes <\/strong><\/h2>\n<p>Adoptive transfer of cytomegalovirus (cmv)-specific t cells has shown promising results in preventing pathological effects caused by opportunistic cmv infection in patients following allogeneic hematopoietic stem cell transplantation. The majority of studies have used steady-state leukapheresis for cmv-reactive product manufacture, a collection obtained prior to or weeks after granulocyte-colony stimulating factor (g-csf) mobilization, but the procurement of this additional sample is often not available in the unrelated donor setting. If the cellular product for adoptive immunotherapy could be generated from the same g-csf mobilized collection, the problems associated with the additional harvest could be overcome. However, the tolerogenic effects induced by g-csf on t cells could affect the anti-viral in vivo functionality of cmv-specific t cells obtained from g-csf mobilized donor samples if they were infused into the patient.  in this thesis it has been investigated the use of g-csf mobilized samples for the manufacture of cmv-specific t cells using two direct selection methods. First, cmv-specific t cells were isolated based on activation-dependent cd137 expression, which demonstrated a high specificity for cmv, the secretion of cytotoxic effector molecules, proliferation in response to the antigen and lysis of cmv-loaded target cells, showing that they shared the same characteristics as cmv-specific t cells obtained from non-mobilized cells. On the other hand, cmv-specific cd8+ t cells were isolated using mhc-multimers and showed that products obtained from g-csf mobilized samples were able to respond to the antigen by expressing activation markers and secreting pro-inflammatory cytokines and lytic molecules, but the in vitro proliferative capacity was impaired.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Evaluation and optimization of immunomagnetic methods for the purification and selection of cmv-specific cytotoxic t lymphocytes from g-csf primed peripheral blood mononuclear cells for adoptive immunotherapy<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Evaluation and optimization of immunomagnetic methods for the purification and selection of cmv-specific cytotoxic t lymphocytes from g-csf primed peripheral blood mononuclear cells for adoptive immunotherapy <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Lorea Beloki Bes <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/12\/2014<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>arostegui Olavarria L\u00f3pez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  jos\u00e9 Lasarte sagastibelza <\/li>\n<li>Jos\u00e9 alejandro Madrigal fern\u00e1ndez (vocal)<\/li>\n<li>mercedes Rodriguez calvillo (vocal)<\/li>\n<li>aurelio Barricarte gurrea (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Lorea Beloki Bes Adoptive transfer of cytomegalovirus (cmv)-specific t cells has shown promising results in preventing pathological [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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