{"id":117503,"date":"2018-03-11T10:47:03","date_gmt":"2018-03-11T10:47:03","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-molecular-proteomico-y-filogenetico-de-la-zona-pelucida-de-ovocitos-de-coneja-oryctolagus-cuniculus-y-gata-felis-catus\/"},"modified":"2018-03-11T10:47:03","modified_gmt":"2018-03-11T10:47:03","slug":"analisis-molecular-proteomico-y-filogenetico-de-la-zona-pelucida-de-ovocitos-de-coneja-oryctolagus-cuniculus-y-gata-felis-catus","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/analisis-molecular-proteomico-y-filogenetico-de-la-zona-pelucida-de-ovocitos-de-coneja-oryctolagus-cuniculus-y-gata-felis-catus\/","title":{"rendered":"An\u00e1lisis molecular, prote\u00f3mico y filogen\u00e9tico de la zona pel\u00facida de ovocitos de coneja (oryctolagus cuniculus) y gata (felis catus)"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Nelida Irene Stetson <\/strong><\/h2>\n<p>1.\tResumen  el oocito de mam\u00edfero est\u00e1 rodeado por una matriz denominada zona pel\u00facida (zp). Esta envoltura participa en procesos tales como la inducci\u00f3n de la reacci\u00f3n acros\u00f3mica, la uni\u00f3n de espermatozoides y tambi\u00e9n puede estar implicada en la especiaci\u00f3n. Estudios recientes han demostrado que la matriz de zp de ovocitos en varias especies se compone de cuatro glicoprote\u00ednas, denominadas zp1, zp2, zp3 y zp4, en lugar de las tres descritas en el rat\u00f3n, cerdo y vaca. En la gata (felis catus), esta matriz se compone de al menos tres glicoprote\u00ednas denominadas zp2, zp3 y zp4. Sin embargo, la presencia de cuatro  prote\u00ednas en varios mam\u00edferos, sugiere que necesita una reevaluaci\u00f3n de la zp en la gata. Adem\u00e1s, en esta tesis, se realizaron investigaciones para determinar la expresi\u00f3n de una cuarta glicoprote\u00edna en la zp de conejo (oryctolagus cuniculis). Los objetivos de esta investigaci\u00f3n fueron analizar la composici\u00f3n de prote\u00ednas de zp gato por medio de an\u00e1lisis prote\u00f3mico y en el conejo se amplific\u00f3 zp1 mediante la reacci\u00f3n en cadena de la polimerasa (rt-pcr). En la zp aislada de ovarios y ovocitos de gato, se detectaron varios p\u00e9ptidos correspondientes a cuatro prote\u00ednas, teniendo una cobertura del 33,17%, 71,50%, 50,23% y 49,64% para zp1, zp2, zp3 y zp4, respectivamente. Por otra parte, se confirm\u00f3 por an\u00e1lisis molecular la expresi\u00f3n de los cuatro genes de zp a partir de arn total aislado de ovarios de gato, las zps de gato fueron parcialmente amplificadas por rt-pcr. En el conejo la zp1 incluye un marco de lectura abierto de 1825 nucle\u00f3tidos que codifican un polip\u00e9ptido de 608 residuos de amino\u00e1cidos. La secuencia de amino\u00e1cidos deducida de zp1 de conejo mostr\u00f3 una alta identidad con otras especies: 70% de identidad con zp1 humana y caballo y 67% de identidad con la zp1 de rat\u00f3n y rata. A nivel prote\u00f3mico, fueron detectados por espectrometr\u00eda de masas p\u00e9ptidos correspondientes a las cuatro prote\u00ednas zp. Adem\u00e1s, mediante un an\u00e1lisis filogen\u00e9tico molecular de zp1 mostr\u00f3 que este gen presenta una pseudogenizaci\u00f3n de por lo menos cuatro veces durante la evoluci\u00f3n de los mam\u00edferos. Los datos presentados en esta tesis proporcionan evidencia, por primera vez, que la zp de conejo y la zp de gato se compone de cuatro glicoprote\u00ednas.                     2. Summary the mammalian oocyte is surrounded by a matrix called the zona pellucida (zp). This envelope participates in processes such as acrosome reaction induction, sperm binding, and may be involved in speciation. Recent studies have shown that the zp matrix of oocytes in several species is composed of four glycoproteins, designated zp1, zp2, zp3 and zp4, rather than the three described in mouse, pig and cow. In cat (felis catus), this matrix is composed of at least three glycoproteins called zp2, zp3 and zp4. However, the presence of a fourth protein in several mammals, meaning that a reevaluation of cat zp is needed. Additionally, in this thesis, investigations were carried out to unveil a fourth glycoprotein in the rabbit (oryctolagus cuniculis) zp. The objectives of this research was to analyse of the protein composition of cat zp by means of proteomic analysis and rabbit zp1 was amplified by reverse transcribed polymerase chain reaction (rt-pcr). Using zp from ovaries and oocytes of cat, several peptides corresponding to four proteins were detected, yielding a coverage of 33.17%, 71.50%, 50.23% and 49.64% for zp1, zp2, zp3 and zp4, respectively. Moreover, the expression of four genes was confirmed by molecular analysis. Using total rna isolated from cat ovaries, the complementary deoxyribonucleic acids (cdnas) encoding cat zps were partially amplified by rt-pcr. In the rabbit the zp1 cdna contains an open reading frame of 1825 nucleotides encoding a polypeptide of 608 amino acid residues. The deduced amino acid sequence of rabbit zp1 showed high identity with other species: 70% identity with human and horse zp1, and 67% identity with mouse and rat zp1. At the proteomic level, peptides corresponding to the four proteins were detected by mass spectrometry. In addition, a molecular phylogenetic analysis of zp1 showed that pseudogenization of this gene has occurred at least four times during the evolution of mammals. The data presented in this thesis provide evidence, for the first time, that the rabbit and cat zp is composed of four glycoproteins.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis molecular, prote\u00f3mico y filogen\u00e9tico de la zona pel\u00facida de ovocitos de coneja (oryctolagus cuniculus) y gata (felis catus)<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis molecular, prote\u00f3mico y filogen\u00e9tico de la zona pel\u00facida de ovocitos de coneja (oryctolagus cuniculus) y gata (felis catus) <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Nelida Irene Stetson <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 22\/05\/2015<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda Jos\u00e9 Izquierdo Rico<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Luis Miguel Pastor garcia <\/li>\n<li>pablo Bermejo \u00e1lvarez (vocal)<\/li>\n<li>Mar\u00eda Arias alvarez (vocal)<\/li>\n<li>Emilio G\u00f3mez s\u00e1nchez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Nelida Irene Stetson 1. Resumen el oocito de mam\u00edfero est\u00e1 rodeado por una matriz denominada zona pel\u00facida [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[849,36483,8235],"tags":[160239,27432,197729,196084,231495,205696],"class_list":["post-117503","post","type-post","status-publish","format-standard","hentry","category-biologia-celular","category-biologia-molecular","category-murcia","tag-emilio-gomez-sanchez","tag-luis-miguel-pastor-garcia","tag-maria-arias-alvarez","tag-maria-jose-izquierdo-rico","tag-nelida-irene-stetson","tag-pablo-bermejo-alvarez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/117503","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=117503"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/117503\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=117503"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=117503"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=117503"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}