{"id":117609,"date":"2015-12-06T00:00:00","date_gmt":"2015-12-06T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-migracion-de-neuronas-pax6-en-el-talamo-del-pollo-y-del-raton\/"},"modified":"2015-12-06T00:00:00","modified_gmt":"2015-12-06T00:00:00","slug":"estudio-de-migracion-de-neuronas-pax6-en-el-talamo-del-pollo-y-del-raton","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/neurociencias\/estudio-de-migracion-de-neuronas-pax6-en-el-talamo-del-pollo-y-del-raton\/","title":{"rendered":"Estudio de migraci\u00f3n de neuronas pax6+ en el t\u00e1lamo del pollo y del rat\u00f3n"},"content":{"rendered":"

Tesis doctoral de Jos\u00e9 Manuel Domenech Hita <\/strong><\/h2>\n

Estudio de migraci\u00f3n de neuronas pax6+ en el t\u00e1lamo del pollo y del rat\u00f3n objetivo general el objetivo general de esta tesis es la caracterizaci\u00f3n de la migraci\u00f3n protagonizada por un conjunto de neuronas pax6+ en el t\u00e1lamo del pollo y del rat\u00f3n y su an\u00e1lisis comparativo. materiales y m\u00e9todos se utilizaron embriones de pollo (hh28 – hh38) para analizar mediante t\u00e9cnicas de inmunohistoqu\u00edmica y de hibridaci\u00f3n in situ con sondas de mrna del gen pax6 en secciones de vibratomo. Tambi\u00e9n se llevaron a cabo marcajes mediante bda y posterior cultivo organot\u00edpico. Las preparaciones fueron escaneadas y analizadas. Las im\u00e1genes de secciones de cerebros de rat\u00f3n procesadas mediante hibridaci\u00f3n in situ para el gen pax6 de estadios embrionarios y postnatales fueron obtenidas del atlas de desarrollo del rat\u00f3n (allen brain atlas). resultados y conclusiones las observaciones obtenidas confirman la existencia de una migraci\u00f3n neuronal tard\u00eda que se origina en la regi\u00f3n retrohabenular, en el techo posterior del pros\u00f3mero 2. las c\u00e9lulas migratorias pax6+ comienzan a visualizarse claramente en la regi\u00f3n retrohabenular en el estadio hh31, terminando la migraci\u00f3n aparentemente en hh37. Se desplazan desde su origen laterorrostralmente en la zona fronteriza entre la regi\u00f3n habenular y el t\u00e1lamo propiamente dicho. En el caso del pollo, este avance es m\u00e1s marcado y alcanza, a trav\u00e9s del n\u00facleo superficial microcelular, regiones cercanas a la frontera intertal\u00e1mica, incorpor\u00e1ndose escasas c\u00e9lulas de esta migraci\u00f3n a la regi\u00f3n habenular lateral. En rat\u00f3n, en cambio, la migraci\u00f3n penetra m\u00e1s importantemente en la regi\u00f3n habenular lateral donde acaba integr\u00e1ndose en el subn\u00facleo parvocelular de la divisi\u00f3n lateral del n\u00facleo habenular lateral y a penas se observan otras c\u00e9lulas fuera de esa zona habenular. en el pollo se observa un segundo origen celular pax6+ a continuaci\u00f3n del \u00e1rea retrohabenular, ya en la zona pretectal precomisural. Desde este origen existe una peque\u00f1a migraci\u00f3n que alcanza aparentemente las inmediaciones del n\u00facleo parvocelular de la comisura posterior. el resultado de los marcajes con bda in vitro en el estadio hh31, de producci\u00f3n inicial de estas c\u00e9lulas, corrobor\u00f3 la existencia de un movimiento migratorio desde este origen en la regi\u00f3n retrohabenular hacia la zona de destino descrita mediante hibridaci\u00f3n in situ de pax6, tanto en el caso de la migraci\u00f3n tal\u00e1mica como la pretectal. desde el punto de vista de la anatom\u00eda comparada, se concluye que los fen\u00f3menos retrohabenulares migratorios observados en el pollo y en el rat\u00f3n coinciden en su cronolog\u00eda embriol\u00f3gica, ya que aparecen en estadios comparables y en ambos casos figura como diana la regi\u00f3n habenular lateral. Si bien en el pollo aparecen c\u00e9lulas que parecen invadir zonas no habenulares cercanas cabe preguntarse si no cabr\u00eda incluir dichas regiones en la zona habenular. En el caso del rat\u00f3n las c\u00e9lulas migradas componen una subpoblaci\u00f3n menor del \u00e1rea habenular lateral que forma el subn\u00facleo parvocelular de la divisi\u00f3n lateral del n\u00facleo habenular lateral. study of the migration of pax6+ neurons in the chick and mouse thalamus general aim the overall objective of this thesis is to characterize the migration of a population of pax6+ neurons in the chick and mouse thalamus and its comparative analysis. methods chick embryos (hh28 – hh38) were used to analyze by immunohistochemistry and in situ hybridization with pax6 mrna probes in vibratome sections. Labelling with bda were also carried out and subsequent organotypic culture. The preparations were scanned and analyzed. The images of mouse brain sections processed by in situ hybridization for the pax6 gene in embryonic and postnatal stages were obtained from the atlas of mouse development (allen brain atlas). results and conclusions the observations confirm the existence of a late neuronal migration originated in the retrohabenular region, in the posterior roof of prosomere 2. migratory pax6+ cells become clearly visible in the retrohabenular region at hh31, finishing the migration apparently at hh37. They progress from their origin latero-rostrally through the habenular-thalamic border. This advance is more pronounced in the chick, reaching through the superficial microcellular nucleus regions near the intrathalamic border, joining few of these migrating cells the lateral habenular region. In contrast, the migration in mouse penetrates mainly in the lateral habenular region where it integrates the parvocellular subnucleus of the lateral division of the lateral habenular nucleus and scarce cells are found out of the habenular region. a second pax6+ cellular origin is found in chick, adjacent to the retrohabenular area, in the precommisural pretectal region. From this origin a small migration takes place apparently to the surroundings of the parvocellular nucleus of the posterior commisure. the results of the in vitro bda labelling at hh31, initial production stage of these cells, confirmed the existence of a migratory movement from this origin in the retrohabenular region to the target area described by pax6 in situ hybridization, for both thalamic and pretectal migrations. from a comparative anatomical point of view, the retrohabenular migratory phenomena observed in chick and mouse concur in their embryological chronology, since they appear at comparable stages and the target is the lateral habenular region in both cases. While in chick there appear cells invading nearby non habenular regions, one might wonder whether these regions should be included in the habenular zone. In the case of mouse, the migrated cells shape a minor subpopulation of the lateral habenular area that forms the parvocellular part of the lateral division of the lateral habenular nucleus.<\/p>\n

 <\/p>\n

Datos acad\u00e9micos de la tesis doctoral \u00abEstudio de migraci\u00f3n de neuronas pax6+ en el t\u00e1lamo del pollo y del rat\u00f3n<\/strong>\u00ab<\/h3>\n
    \n
  • T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de migraci\u00f3n de neuronas pax6+ en el t\u00e1lamo del pollo y del rat\u00f3n <\/li>\n
  • Autor:<\/strong>\u00a0 Jos\u00e9 Manuel Domenech Hita <\/li>\n
  • Universidad:<\/strong>\u00a0 Murcia<\/li>\n
  • Fecha de lectura de la tesis:<\/strong>\u00a0 12\/06\/2015<\/li>\n<\/ul>\n

     <\/p>\n

    Direcci\u00f3n y tribunal<\/h3>\n
      \n
    • Director de la tesis<\/strong>\n
        \n
      • Luis Puelles Lopez<\/li>\n<\/ul>\n<\/li>\n
      • Tribunal<\/strong>\n
          \n
        • Presidente del tribunal: margarita Martinez de la torre fox <\/li>\n
        • Mar\u00eda del pilar Aroca tejedor (vocal)<\/li>\n
        • carmen D\u00edaz delgado (vocal)<\/li>\n
        • diego Echevarria aza (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          Tesis doctoral de Jos\u00e9 Manuel Domenech Hita Estudio de migraci\u00f3n de neuronas pax6+ en el t\u00e1lamo del pollo y del […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[4983,8235,4275],"tags":[55900,212938,231661,1823,216433,135379],"class_list":["post-117609","post","type-post","status-publish","format-standard","hentry","category-embriologia-animal","category-murcia","category-neurociencias","tag-carmen-diaz-delgado","tag-diego-echevarria-aza","tag-jose-manuel-domenech-hita","tag-luis-puelles-lopez","tag-margarita-Martinez-de-la-torre-fox","tag-maria-del-pilar-aroca-tejedor"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/117609","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=117609"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/117609\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=117609"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=117609"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=117609"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}