{"id":117841,"date":"2015-10-07T00:00:00","date_gmt":"2015-10-07T00:00:00","guid":{"rendered":""},"modified":"2015-10-07T00:00:00","modified_gmt":"2015-10-07T00:00:00","slug":"embryo-development-in-vitro-in-cattele-role-of-oviduct-cells-oviductal-fluid-and-extracellular-vesicles-desarrollo-embrionario-in-vitro-en-bovino-funcion-de-las-celulas-del-oviducto-fluido-oviductal","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-veterinarias\/embryo-development-in-vitro-in-cattele-role-of-oviduct-cells-oviductal-fluid-and-extracellular-vesicles-desarrollo-embrionario-in-vitro-en-bovino-funcion-de-las-celulas-del-oviducto-fluido-oviductal\/","title":{"rendered":"Embryo development in vitro in cattele role of oviduct cells oviductal fluid and extracellular vesicles\/\/desarrollo embrionario in vitro en bovino funci\u00f3n de las c\u00e9lulas del oviducto fluido oviductal y ves\u00edculas extracelulares"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ricaurte Lopera V\u00e1squez <\/strong><\/h2>\n<p>El conocimiento del oviducto bovino y del desarrollo embrionario preimplantacional han permitido la consolidaci\u00f3n de t\u00e9cnicas como la producci\u00f3n in vitro de embriones, que alimitar las condiciones fisiol\u00f3gicas, se ha convertido en una valiosa herramienta en esquemas de mejora gen\u00e9tica y en investigaci\u00f3n. Sin embargo, a\u00fan presenta desventajas como la bajacantidad y calidad de los embriones obtenidos.El uso de c\u00e9lulas epiteliales oviductales (boec) en sistemas de cultivo in vitro ha permitido descubrir sustancias embriotr\u00f3ficas y mecanismos de comunicaci\u00f3n materno-embrionario. A su vez, tanto el fluido oviductal (of), como componente principal del ambiente oviductal, como las ves\u00edculas extracelulares (ev), como secreciones de las c\u00e9lulas som\u00e1ticas y que act\u00faan como mediadoras del transporte intercelular, pueden desempe\u00f1ar un importante rol reproductivo a nivel preimplantacional. Por lo tanto, el objetivo de la tesis fue evaluar el efectode las boec, el of y las ev sobre el desarrollo y la calidad de los embriones bovinos producidos in vitro. En un primer experimento se evalu\u00f3 el efecto de distintos sistemas decultivo con boec y con sus medios condicionados y se estandariz\u00f3 un protocolo para la obtenci\u00f3n y caracterizaci\u00f3n de las ev. En un segundo experimento se evidenci\u00f3 que la suplementaci\u00f3n con of a bajas concentraciones durante el cultivo in vitro incrementan eldesarrollo y la calidad de los blastocistos, y modifican su patrones de expresi\u00f3n g\u00e9nica. En untercer experimento se caracterizaron las ev presentes en el of y se demostr\u00f3 que en particularlas provenientes del istmo incrementan la calidad, y modifican la expresi\u00f3n g\u00e9nica de losblastocistos. Los resultados obtenidos evidencian una asociaci\u00f3n esencial entre el ambienteoviductal (c\u00e9lulas-fluido-ev) y el embri\u00f3n en desarrollo, confirmando un di\u00e1logo materno-embrionario en la especie bovina.Palabras clave:in vitro, desarrollo embrionario, calidad embrionaria, c\u00e9lulas epiteliales deloviducto, fluido oviductal, ves\u00edculas extracelulares, bovino.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Embryo development in vitro in cattele role of oviduct cells oviductal fluid and extracellular vesicles\/\/desarrollo embrionario in vitro en bovino funci\u00f3n de las c\u00e9lulas del oviducto fluido oviductal y ves\u00edculas extracelulares<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Embryo development in vitro in cattele role of oviduct cells oviductal fluid and extracellular vesicles\/\/desarrollo embrionario in vitro en bovino funci\u00f3n de las c\u00e9lulas del oviducto fluido oviductal y ves\u00edculas extracelulares <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ricaurte Lopera V\u00e1squez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 10\/07\/2015<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Miguel \u00e1ngel Ram\u00edrez De Paz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: pedro Luis Lorenzo gonzalez <\/li>\n<li>bel\u00e9n Pintado sanJuan benito (vocal)<\/li>\n<li>Jos\u00e9 s. Vicente ant\u00f3n (vocal)<\/li>\n<li>Manuel Aviles sanchez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ricaurte Lopera V\u00e1squez El conocimiento del oviducto bovino y del desarrollo embrionario preimplantacional han permitido la consolidaci\u00f3n [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[515,986],"tags":[9140,198018,129207,197657,148749,231990],"class_list":["post-117841","post","type-post","status-publish","format-standard","hentry","category-ciencias-veterinarias","category-complutense-de-madrid","tag-belen-pintado-sanjuan-benito","tag-jose-s-vicente-anton","tag-manuel-aviles-sanchez","tag-miguel-angel-ramirez-de-paz","tag-pedro-luis-lorenzo-gonzalez","tag-ricaurte-lopera-vasquez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/117841","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=117841"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/117841\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=117841"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=117841"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=117841"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}