{"id":117844,"date":"2015-10-07T00:00:00","date_gmt":"2015-10-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/efecto-protector-del-te-blanco-frente-al-estres-oxidativo-agudo-en-ratas-mecanismos-geneticos-y-moleculares\/"},"modified":"2015-10-07T00:00:00","modified_gmt":"2015-10-07T00:00:00","slug":"efecto-protector-del-te-blanco-frente-al-estres-oxidativo-agudo-en-ratas-mecanismos-geneticos-y-moleculares","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/fisiologia-animal\/efecto-protector-del-te-blanco-frente-al-estres-oxidativo-agudo-en-ratas-mecanismos-geneticos-y-moleculares\/","title":{"rendered":"Efecto protector del t\u00e9 blanco frente al estr\u00e9s oxidativo agudo en ratas. mecanismos gen\u00e9ticos y moleculares"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Crist\u00f3bal Espinosa Ruiz <\/strong><\/h2>\n<p>Antecedentes: las propiedades beneficiosas del t\u00e9 (camelia sinensis) son ampliamente conocidas. El t\u00e9 posee una gran capacidad antioxidante debida  la presencia de polifenoles denominados catequinas. Estas catequinas poseen diversas propiedades que le permiten ejercer ciertos efectos de protecci\u00f3n contra agentes oxidantes m\u00e1s all\u00e1 de su capacidad antioxidante. Existen pocos estudios realizados a largo plazo y que, adem\u00e1s, sean realizados con dosis similares a las que se ingieren normalmente en la sociedad. el factor de trascripci\u00f3n nrf2, se encuentra en el citoplasma unido a un represor (keap1) del cual se separa en condiciones de estr\u00e9s oxidativo y penetra en el n\u00facleo activando una serie de genes de respuesta antioxidante (respuesta are) entre los que se incluyen diversas enzimas antioxidantes y detoxificantes. Existen estudios que sugieren que algunas catequinas podr\u00edan activar esta respuesta. la adriamicina es un antibi\u00f3tico de la familia de las antraciclinas, aislado de streptomices peucetis hace m\u00e1s de 50 a\u00f1os. Posee la capacidad de detener la divisi\u00f3n celular, y por ello es utilizado contra una gran variedad de canceres y tumores. Tambi\u00e9n tiene una gran capacidad de generar radicales libres in vivo. Los pacientes tratados con esta sustancia sufren da\u00f1os en todos los \u00f3rganos, en especial en el coraz\u00f3n debido a que esta mol\u00e9cula tiene gran afinidad con la cardiolipina. Se han realizados numerosos estudios para intentar neutralizar los efectos secundarios de este f\u00e1rmaco, con el fin de aumentar la supervivencia de los pacientes e incrementar la calidad del tratamiento.  objetivos: por ello se pretende estudiar los efectos de la ingesta de t\u00e9, as\u00ed como la capacidad que posee el mismo para proteger a los tejidos de una situaci\u00f3n de estr\u00e9s oxidativo agudo.  materiales y m\u00e9todo: para ello, realizaremos primero un estudio en ratas de la raza sprague dawley en jaulas metab\u00f3licas que analice las posibles interferencias con la dieta de una ingesta continuada de t\u00e9 blanco.  posteriormente, comenzaremos con un tratamiento con diferentes dosis de t\u00e9 blanco (0, 15 y 45 mg de extracto de t\u00e9 blanco\/kg de peso corporal al d\u00eda que equivalen a 0, 4 y 12 mg de polifenoles \/kg de peso corporal al d\u00eda) en el agua de bebida. Tras 12 meses de tratamiento se administran a todas  las ratas (excepto a la mitad de los animales control a los cuales se les inyecta soluci\u00f3n salina) una inyecci\u00f3n intraperitoneal de adriamicina (10mg \/kg de peso corporal). Tras 48 horas se anestesian los animales se sacrifican obteni\u00e9ndose  muestras de sangre y tejidos (h\u00edgado, coraz\u00f3n, cerebro y ri\u00f1\u00f3n). Se obtuvo la fracci\u00f3n microsomal de algunos de ellos (h\u00edgado, coraz\u00f3n y cerebro) y el plasma. Se realizaron diferentes an\u00e1lisis: como la medici\u00f3n de la actividad antioxidante, los grupos carbonilo e hidroper\u00f3xidos. Adem\u00e1s, se realiz\u00f3 un an\u00e1lisis de expresi\u00f3n g\u00e9nica en h\u00edgado y ri\u00f1\u00f3n de genes relacionados con el nrf2 y enzimas detoxificantes y antioxidantes. Se midieron tambi\u00e9n la actividad de las enzimas antioxidantes. Se realizaron tambi\u00e9n an\u00e1lisis en los perfiles de \u00e1cidos grasos del coraz\u00f3n y el h\u00edgado.  resultados y discusi\u00f3n: el t\u00e9 no interfiere en la eficiencia alimentaria de las ratas estudiadas, solo se encontr\u00f3 una ligera disminuci\u00f3n en la absorci\u00f3n del fe.  por otro lado, los resultados indicaron que la ingesta a largo plazo de t\u00e9 blanco protegi\u00f3 contra los efectos oxidantes de la adriamicina en todos los tejidos y \u00f3rganos analizados activando adem\u00e1s la repuesta are v\u00eda nrf2 en el h\u00edgado y no siendo necesaria la activaci\u00f3n de la misma en el ri\u00f1\u00f3n. Estudios adicionales son necesarios para comprender todos los mecanismos implicados en la respuesta frente a un agente como la adriamicina. No se encontraron diferencias en los perfiles de \u00e1cidos grasos.    background: the beneficial properties of tea (camellia sinensis) are widely known. The tea has a high antioxidant activity due the presence of polyphenols called catechins. catechins have various properties that allow you to exert some protective effects against oxidizing agents beyond their antioxidant capacity. There are few long-term studies and also are made with doses similar to those normally consumed in society. the transcription factor nrf2 is located in the cytoplasm bound to a repressor (keap1). This union is separated in conditions of oxidative stress and nrf2 enters the core by activating a series of antioxidant response genes (are) between those included various antioxidant and detoxifying enzymes. Studies suggest that some catechins could activate this response. adriamycin is an antibiotic of the anthracycline family, isolated from streptomyces peucetis over 50 years. It has the ability to stop cell division and therefore is used against a variety of cancers and tumors. It also has a great ability to generate free radicals in vivo. Patients treated with this substance suffer damage in all organs, especially the heart because adriamycin has great affinity with cardiolipin. Numerous studies have been undertaken to try to counteract the side effects of this drug, in order to increase patient survival and increase quality of treatment.  objectives: the aim of the present thesis is to study the different effects of white tea intake, and the ability to possess the same to protect tissues from a situation of acute oxidative stress.  matherials and method: with this purpose, we studied sprague dawley in metabolic cages to analyze the potential interference with an ongoing dietary intake of white tea. later, we began a treatment with different doses of white tea (0, 15 and 45 mg of extract of white \/ kg body weight tea a day, equivalent to 0, 4 and 12 mg of polyphenols \/ kg of body weight per day) in the drinking water. After 12 months of treatment all rats was administered (except to half of control animals which are injected with saline) with an intraperitoneal injection of adriamycin (10mg \/ kg body weight). After 48 hours the animals are anesthetized and sacrificed, obtaining samples of blood and tissues (liver, heart, brain and kidney). The microsomal fraction of some organs (liver, heart and brain) and plasma was obtained. Different analyzes were realized: as antioxidant activity, hydroperoxides and carbonyl groups. Furthermore, gene expression in liver and kidney related nrf2 and detoxifying enzymes and antioxidant genes was measured. The activity of antioxidant enzymes was also analyzed. The fatty acid profiles of the heart and liver were measured.  results and discussion: tea not interferes with the feed efficiency of the rats studied; we only found a slight decrease in the fe absorption. moreover, the results indicated that the long term intake of white tea protected against oxidative effects of adriamycin in all tissues and organs analyzed further activating are via nrf2 response in the liver and not activating it being necessary kidney. Additional studies are needed to understand all the mechanisms involved in the response to an agent such as adriamycin. No differences in fatty acid profiles were found.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Efecto protector del t\u00e9 blanco frente al estr\u00e9s oxidativo agudo en ratas. mecanismos gen\u00e9ticos y moleculares<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Efecto protector del t\u00e9 blanco frente al estr\u00e9s oxidativo agudo en ratas. mecanismos gen\u00e9ticos y moleculares <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Crist\u00f3bal Espinosa Ruiz <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 10\/07\/2015<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 \u00e1ngel L\u00f3pez Jim\u00e9nez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: salvador Zamora navarro <\/li>\n<li>Jos\u00e9 enrique Campillo \u00e1lvarez (vocal)<\/li>\n<li>M\u00aa pilar Almajano pablos (vocal)<\/li>\n<li>Juli\u00e1n Castillo sanchez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Crist\u00f3bal Espinosa Ruiz Antecedentes: las propiedades beneficiosas del t\u00e9 (camelia sinensis) son ampliamente conocidas. El t\u00e9 posee [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[34275,675,8235],"tags":[231993,119370,1743,124291,206510,4252],"class_list":["post-117844","post","type-post","status-publish","format-standard","hentry","category-antioxidantes-en-los-alimentos","category-fisiologia-animal","category-murcia","tag-cristobal-espinosa-ruiz","tag-jose-angel-lopez-jimenez","tag-jose-enrique-campillo-alvarez","tag-julian-castillo-sanchez","tag-ma-pilar-almajano-pablos","tag-salvador-zamora-navarro"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/117844","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=117844"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/117844\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=117844"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=117844"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=117844"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}