{"id":117892,"date":"2018-03-11T10:47:38","date_gmt":"2018-03-11T10:47:38","guid":{"rendered":""},"modified":"2018-03-11T10:47:38","modified_gmt":"2018-03-11T10:47:38","slug":"la-caracterizacion-funcional-de-un-mutante-de-insercion-de-tomate-solanum-lycopersicum-identifica-un-factor-de-transcripcion-myb-implicado-en-el-cierre-estomatico-y-transporte-de-na-en-condiciones","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-agrarias\/la-caracterizacion-funcional-de-un-mutante-de-insercion-de-tomate-solanum-lycopersicum-identifica-un-factor-de-transcripcion-myb-implicado-en-el-cierre-estomatico-y-transporte-de-na-en-condiciones\/","title":{"rendered":"La caracterizacion funcional de un mutante de insercion de tomate (solanum lycopersicum) identifica un factor de transcripcion myb implicado en el cierre estomatico y transporte de na+ en condiciones salinas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Juan  Francisco Campos Serrano <\/strong><\/h2>\n<p>A pesar de la relevancia econ\u00f3mica del tomate, los mecanismos que gobiernan la respuesta a la salinidad en esta especie hort\u00edcola no est\u00e1n bien caracterizados y, por consiguiente, la identificaci\u00f3n de genes implicados en la tolerancia del tomate a la salinidad es un objetivo prioritario. Dentro del programa de mutag\u00e9nesis insercional que se est\u00e1 desarrollando entre tres grupos de investigaci\u00f3n (ibmcp-Valencia, universidad almer\u00eda y cebas), se ha generado una colecci\u00f3n de l\u00edneas de tomate (solanum lycopersicum) a partir del cv moneymaker). Con el fin de identificar genes implicados en la tolerancia del tomate a la salinidad, se procedi\u00f3 a la evaluaci\u00f3n de diferentes l\u00edneas de esta colecci\u00f3n y tras la identificaci\u00f3n de algunos mutantes de inter\u00e9s, este trabajo se ha centrado en la caracterizaci\u00f3n del mutante ars1 (altered response to salt stress 1), que muestra sensibilidad al estr\u00e9s salino a lo largo del ciclo vegetativo y se manifiesta en una reducci\u00f3n del rendimiento en fruto del mutante, respecto al cv moneymaker (wt). La caracterizaci\u00f3n molecular por el grupo de la ual ha permitido clonar el gen anulado en el mutante, un factor de transcripci\u00f3n myb de tipo r1 (ars1), siendo el primer gen de este tipo identificado en tomate. En este trabajo se ha demostrado que el mutante ars1 est\u00e1 afectado en el estr\u00e9s i\u00f3nico inducido por la salinidad, reflejado en una mayor acumulaci\u00f3n de na+ en las hojas. Sin embargo, el mayor transporte de na+ a la parte a\u00e9rea del mutante parece ser una consecuencia de su mayor p\u00e9rdida de agua v\u00eda transpiraci\u00f3n, ya que el cierre estom\u00e1tico es mayor en el mutante ars1 que en wt en condiciones de salinidad. Con objeto de comprobar si el principal efecto provocado por la anulaci\u00f3n del gen ars1 era la mayor p\u00e9rdida de agua en situaciones de estr\u00e9s, se estudi\u00f3 la respuesta fisiol\u00f3gica del wt y mutante a la deshidrataci\u00f3n. Los resultados corroboran una mayor deshidrataci\u00f3n del mutante frente al estr\u00e9s debida a su menor capacidad para reducir la conductancia estom\u00e1tica y transpiraci\u00f3n. Adem\u00e1s, la desregulaci\u00f3n del cierre estom\u00e1tico del mutante ars1 en condiciones de estr\u00e9s parece ser dependiente de aba, ya que el mutante muestra mayor apertura estom\u00e1tica cuando se aplica aba ex\u00f3genamente. Finalmente, la caracterizaci\u00f3n funcional de l\u00edneas de sobreexpresi\u00f3n y silenciamiento del gen ars1 demuestran el papel de este gen en la regulaci\u00f3n de la p\u00e9rdida de agua v\u00eda transpiraci\u00f3n en condiciones de estr\u00e9s.  despite the economic relevance of the tomato, the mechanisms that govern the response to salinity in this horticultural species are not well characterized and therefore, the identification of genes involved in the tolerance of tomato to salinity is a priority. In the program of insertional mutagenesis developed by three research groups (ibmcp-Valencia, almeria university and cebas) it has been generated a collection of lines of tomato (solanum lycopersicum) from cv moneymaker. In order to identify genes involved in the tolerance of tomato to salinity, we proceeded to the evaluation of different lines of this collection and after identifying some mutants of interest, this work has focused on the characterization of mutant ars1 (altered response to salt stress 1), showing sensitivity to salt stress along the growth cycle and manifests itself in reduced fruit yield, compared with to cv moneymaker (wt). The molecular characterization of the ual group has allowed cloning the knock-out gene, a myb transcription factor type r1 (ars1), in mutant, being the first gene of this type identified in tomato. This work has shown that the ars1 mutant is affected in ionic stress induced by salinity reflected in a greater accumulation of na+ in the leaves. However, the increased transport of na+ to the shoot of the mutant seems to be a consequence of their higher water loss via transpiration, since due to stomatal closure is greater in the ars1 mutant than in wt in saline conditions. In order to check if the main effect caused by the knock-out of ars1 gene was the higher water loss under stress, the physiological response of wt and mutant to dehydration was studied. The results support higher dehydration in mutant under stress due to their lower reduced ability to reduce stomatal conductance and transpiration. In addition, deregulation of ars1 mutant stomatal closure under stress appears to be aba-dependent, as the mutant shows greater stomatal aperture when exogenously applied aba. Finally, the functional characterization of overexpression and gene silencing lines demonstrate the role of this gene in regulating water loss via transpiration under stress.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>La caracterizacion funcional de un mutante de insercion de tomate (solanum lycopersicum) identifica un factor de transcripcion myb implicado en el cierre estomatico y transporte de na+ en condiciones salinas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 La caracterizacion funcional de un mutante de insercion de tomate (solanum lycopersicum) identifica un factor de transcripcion myb implicado en el cierre estomatico y transporte de na+ en condiciones salinas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Juan  Francisco Campos Serrano <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 15\/07\/2015<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda Del Carmen Bolar\u00edn Jim\u00e9nez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: manuel Acosta echeverria <\/li>\n<li>nieves Fern\u00e1ndez Garc\u00eda (vocal)<\/li>\n<li>  (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Juan Francisco Campos Serrano A pesar de la relevancia econ\u00f3mica del tomate, los mecanismos que gobiernan la 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