{"id":12253,"date":"2018-03-09T08:58:01","date_gmt":"2018-03-09T08:58:01","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estructura-procesamiento-proteolitico-y-activacion-de-la-proteina-f-del-virus-respiratorio-sincitial-humano\/"},"modified":"2018-03-09T08:58:01","modified_gmt":"2018-03-09T08:58:01","slug":"estructura-procesamiento-proteolitico-y-activacion-de-la-proteina-f-del-virus-respiratorio-sincitial-humano","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/estructura-procesamiento-proteolitico-y-activacion-de-la-proteina-f-del-virus-respiratorio-sincitial-humano\/","title":{"rendered":"Estructura, procesamiento proteolitico y activacion de la proteina f del virus respiratorio sincitial humano"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Luis Gonzalez Reyes <\/strong><\/h2>\n<p>La glicoproteina f del virus respiratorio sincitial humano fusiona las membranas viral y celular. La proteina f y un mutante al que se han eliminado la region transmembrana y citoplasmatica (f tm-) se expresaron a partir de vaccinias recombinantes y se purificaron por cromatografia de inmunoafinidad. mediante el analisis del comportamiento en sds-page, reactividad frente a anticuerpos monoclonales, susceptibilidad a digestion con tripsina, comportamiento en ultracentrifugacion en gradientes de sacarosa y microscopia electronica, se determino que las proteinas f y f tm- tienen una estructura similar, por lo que el ectodominio de la proteina se pliega independientemente.  mediante microscopia electronica se identificaron dos conformaciones de la proteina que, probablemente, correspondan a las formas pre- y post-activas. estudios de inmunomicroscop\u00eda electronica de la proteina f dieron informaci\u00f3n de la localizaci\u00f3n en la estructura tridimensional de los sitios antig\u00e9nicos de la proteina.  la caracterizaci\u00f3n de la forma sin procesar (fo), parcialmente procesada (f a1-109) y totalmente procesada (f1+f2) de las proteinas f tm- y f mediante reactividad frente a sueros especificos, secuenciacion n-terminal y espectrometria de masas, demostro que la proteina f sufre un doble procesamiento proteol\u00edtico durante su maduraci\u00f3n (tras el residuo 109, sitio i y el residuo 136, sitio ii). El corte en ambos sitios es independiente, como se determin\u00f3 mediante mutagenesis dirigida, pero posiblemente primero se produzca en el sitio i. Adem\u00e1s, por ensayos de inmunoprecipitacion con un suero especifico de las formas fo y fa1-109, se determin\u00f3 que f1+f2, y f0 y\/o f a1-109 pueden cooligomerizar en el trimero de proteina f tm-y que el peptido entre los dos sitos de procesamiento no se encuentra en la proteina madura.  por ultimo, preparaciones de proteina f tm-procesadas en el sitio ii aparecieron con la conformacion post-activa, lo que sugiere que el<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estructura, procesamiento proteolitico y activacion de la proteina f del virus respiratorio sincitial humano<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estructura, procesamiento proteolitico y activacion de la proteina f del virus respiratorio sincitial humano <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Luis Gonzalez Reyes <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/07\/2001<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Antonio Melero Fondevilla<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 gregorio Gavilanes franco <\/li>\n<li>Luis Menendez arias (vocal)<\/li>\n<li>Jos\u00e9 Lopez carrascosa (vocal)<\/li>\n<li>mauricio Garcia mateu (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Luis Gonzalez Reyes La glicoproteina f del virus respiratorio sincitial humano fusiona las membranas viral y celular. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483,36606,160,115,35,3178,17,280,15363],"tags":[14396,5832,21426,40100,40101,5595],"class_list":["post-12253","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","category-biologia-molecular-de-microorganismos","category-bioquimica","category-bioquimica-molecular","category-ciencias-de-la-vida","category-proteinas","category-quimica","category-virologia","category-virus-respiratorios","tag-jose-antonio-melero-fondevilla","tag-jose-gregorio-gavilanes-franco","tag-jose-lopez-carrascosa","tag-luis-gonzalez-reyes","tag-luis-menendez-arias","tag-mauricio-garcia-mateu"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/12253","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=12253"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/12253\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=12253"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=12253"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=12253"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}