{"id":12732,"date":"2018-03-09T08:58:42","date_gmt":"2018-03-09T08:58:42","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-de-colinesterasa-en-sangre-entera-de-diferentes-especies-animales-estudios-de-validacion-anala%c2%adtica-y-cla%c2%adnica\/"},"modified":"2018-03-09T08:58:42","modified_gmt":"2018-03-09T08:58:42","slug":"analisis-de-colinesterasa-en-sangre-entera-de-diferentes-especies-animales-estudios-de-validacion-anala%c2%adtica-y-cla%c2%adnica","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-agrarias\/analisis-de-colinesterasa-en-sangre-entera-de-diferentes-especies-animales-estudios-de-validacion-anala%c2%adtica-y-cla%c2%adnica\/","title":{"rendered":"An\u00e1lisis de colinesterasa en sangre entera de diferentes especies animales: estudios de validaci\u00f3n anal\u00edtica y cl\u00ednica"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Fernando Tecles Vicente <\/strong><\/h2>\n<p>El objetivo de este trabajo consisti\u00f3 en la validaci\u00f3n del an\u00e1lisis de la actividad colinesterasa en sangre entera de diferentes especies animales, y en el estudio de la utilidad cl\u00ednica de esta determinaci\u00f3n para detectar la exposici\u00f3n de los animales dom\u00e9sticos a insecticidas organofosforados y carbamatos.  se trat\u00f3 de estudiar la influencia de algunas de las posibles fuentes de variaci\u00f3n sobre la determinaci\u00f3n espectrofotom\u00e9trica de clonesterasa en sangre entera. Un incremento en la temperatura de reacci\u00f3n proporcion\u00f3 una mayor actividad colinesterasa. Se obtuvo una mayor actividad enzim\u00e1tica conforme aument\u00f3 el ph y la concentraci\u00f3n de sustrato utilizada, excepto con una concentraci\u00f3n de atci de 10&#215;10-3m, con la que se observ\u00f3 un descenso de actividad; sin embargo, la hidr\u00f3lisis no enzim\u00e1tica del sustrato se elev\u00f3 r\u00e1pidamente si el ph o la concentraci\u00f3n de sustrato exced\u00edan de 7.5 y 1&#215;10-3 m, respectivamente. Se recomend\u00f3 el empleo de 4 a 8 ciclos de lectura de absorbancia de entre 30 y 90 segundos cada uno, ya que el uso de 3 ciclos, o una duraci\u00f3n de 15 segundos, provoc\u00f3 un descenso de actividad. la heparina result\u00f3 como el anticoagulante de elecci\u00f3n para la obtenci\u00f3n de muestras de sangre destinadas a la determinaci\u00f3n de colinesterasa. Todos los reactivos (los crom\u00f3foros 2-pds y dtnb y los sustratos acetil, butiril y propioniltiocolina) pueden ser conservados en la congelaci\u00f3n por un tiempo de hasta tres meses. Las muestras de sangre entera pueden ser congeladas y descongeladas de forma sucesiva sin sufrir p\u00e9rdida de actividad.  la caracterizaci\u00f3n de la actividad colinesterasa de la snagre fue estudiada mediante el empleo de los sustratos acetil, butiril y propinoniltiocolina para la determianci\u00f3n de colinesterasa en sangre entera de 10 animales adultos de las especies canina, felina, equin, bovina, ovina, caprina y procina. En rumiantes y cerdo, la acetilticolina proporcion\u00f3 la mayor actividad,<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis de colinesterasa en sangre entera de diferentes especies animales: estudios de validaci\u00f3n anal\u00edtica y cl\u00ednica<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis de colinesterasa en sangre entera de diferentes especies animales: estudios de validaci\u00f3n anal\u00edtica y cl\u00ednica <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Fernando Tecles Vicente <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 14\/09\/2001<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>C\u00e1ndido Guti\u00e9rrez Panizo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Luis Benedito castellote <\/li>\n<li>pedro Luis Tornel osorio (vocal)<\/li>\n<li>dolores Ferrando Mar\u00eda (vocal)<\/li>\n<li> Das candeias guilhermino marinho lucia Mar\u00eda (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Fernando Tecles Vicente El objetivo de este trabajo consisti\u00f3 en la validaci\u00f3n del an\u00e1lisis de la actividad [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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