{"id":134017,"date":"1997-01-01T00:00:00","date_gmt":"1997-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/genes-y-enzimas-implicados-en-los-ultimos-pasos-de-la-biosintesis-de-cefalosporina-c\/"},"modified":"1997-01-01T00:00:00","modified_gmt":"1997-01-01T00:00:00","slug":"genes-y-enzimas-implicados-en-los-ultimos-pasos-de-la-biosintesis-de-cefalosporina-c","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/genes-y-enzimas-implicados-en-los-ultimos-pasos-de-la-biosintesis-de-cefalosporina-c\/","title":{"rendered":"Genes y enzimas implicados en los ultimos pasos de la biosintesis de cefalosporina c."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Javier Velasco Alvarez <\/strong><\/h2>\n<p>En este trabajo nos propusimos afrontar el estudio de los siguientes aspectos relacionados con la biosintesis de cefalospirina c: 1. Se estudio el efecto de la adicion de metionina sobre la produccion de este antibiotico y el posible origen de este efecto a nivel enzimatico y genetico demostrando que la metionina aumenta principalmente el nivel de transcripcion de los genes del \u00abcluster\u00bb temprano de biosintesis de cefalosporina c.  2. Se analizaron los niveles relativos de transcripcion de los 4 genes clonados de la ruta biosintetica en tres cepas de diferente nivel de produccion. Se comprobo asi que los genes pcbc y cefef son los que demuestran niveles de expresion mayores, el pcbab se expresa a un nivel algo inferior y el cefg presenta una expresion muy escasa, similar al pyrg del metabolismo primario.  3. Se realizaron estudios bioquimicos sobre la naturaleza del producto del gen cefg de a.Crysogenum que resulto de ser una proteina monomerica de aproximadamente 50kda.  4. Se caracterizaron cepas transformantes de este hongo en las que el gen cefg se habia superexpresado. Estas cepas mostraron mayor actividad dac-acetiltransferasa y mejor capacidad de conversion de dac en cefalosporina c.  5. Por ultimo se purificaron y caracterizaron dos proteinas extracelulares con actividad cpc-acetilhidrolasa. Ambas proteinas aparecen en las ultimas horas de fermentacion y tienen escasa afinidad por la cefalosporina c.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Genes y enzimas implicados en los ultimos pasos de la biosintesis de cefalosporina c.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Genes y enzimas implicados en los ultimos pasos de la biosintesis de cefalosporina c. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Javier Velasco Alvarez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1997<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Francisco Mart\u00edn Mart\u00edn<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Tom\u00e1s Gonz\u00e1lez Villa <\/li>\n<li>Javier Avalos Cordero (vocal)<\/li>\n<li>Paloma Liras Pad\u00edn (vocal)<\/li>\n<li>Fernando Leal Sanchez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Javier Velasco Alvarez En este trabajo nos propusimos afrontar el estudio de los siguientes aspectos relacionados con [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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