{"id":134341,"date":"1997-01-01T00:00:00","date_gmt":"1997-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/regulacion-de-sintesis-de-enzimas-pecticas-en-fusarium-oxysporum-f-sp-radicis-lycopersici-purificacion-y-caracterizacion-de-pectin-y-pectato-liasas\/"},"modified":"1997-01-01T00:00:00","modified_gmt":"1997-01-01T00:00:00","slug":"regulacion-de-sintesis-de-enzimas-pecticas-en-fusarium-oxysporum-f-sp-radicis-lycopersici-purificacion-y-caracterizacion-de-pectin-y-pectato-liasas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/regulacion-de-sintesis-de-enzimas-pecticas-en-fusarium-oxysporum-f-sp-radicis-lycopersici-purificacion-y-caracterizacion-de-pectin-y-pectato-liasas\/","title":{"rendered":"Regulacion de sintesis de enzimas pecticas en fusarium oxysporum f. sp. radicis-lycopersici. purificacion y caracterizacion de pectin y pectato liasas."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Guevara Morato M. Angeles <\/strong><\/h2>\n<p>En esta tesis se han estudiado las enzimas pecticas de fusarium oxysporum f.Sp. Radicis-lycopersici (forl).  inicialmente se analizo la sintesis de distintas polisacaridasas (poligalacturonasa, pectin liasa, beta-1,4-glucanasa, avicelasa y beta-glucosidasa), para a continuacion centrarse en las condiciones optimas de cultivo para la produccion de las enzimas pecticas y caracterizacion de las mismas.  la regulacion de las enzimas pecticas de forl (pectin liasa, pectato liasa y poligalacturonasa) se ha estudiado valorando las actividades enzimaticas, los patrones de expresion genica mediante traducciones in vitro y posteriores electroforesis y con la utilizacion de sondas especificas. Se ha demostrado una induccion por acido galacturonico y represion catabolica por glucosa. A diferencia de los resultados de otros autores no se ha detectado mayor produccion de liasas en los cultivos a ph 8,0. Se han detectado tres formas con actividad liasa, con pis de 9,20, 9,00 y 8,65, y cuatro con actividad poligalacturonasa, la principal con pi 7,35, dos con pis 6,85 y 6,55, y una minoritaria con pi 9,30. Las tres liasas actuan sobre pectina y pectato en condiciones alcalinas y tienen un absoluto requerimiento de calcio.  la mas alcalina tiene preferencia por pectina, mientras que las otras dos por pectato. Las poligalacturonasas son mas activas a ph acido y son inhibidas por calcio. La pectin liasa tiene un comportamiento de tipo \u00abendo\u00bb mientras que las pectato liasa y poligalacturonasa tienen un comportamiento de tipo \u00abexo\u00bb.  por ultimo se han purificado y caracterizado las tres liasas demostrandose un efecto sinergistico entre las dos pectato liasas. La adicion de una fraccion rica en pectin metilesterasa aumento la actividad de las tres liasas en presencia de pectina, lo que parece indicar que la desmetilacion de la pectina favorece la accion de las liasas.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Regulacion de sintesis de enzimas pecticas en fusarium oxysporum f. sp. radicis-lycopersici. purificacion y caracterizacion de pectin y pectato liasas.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Regulacion de sintesis de enzimas pecticas en fusarium oxysporum f. sp. radicis-lycopersici. purificacion y caracterizacion de pectin y pectato liasas. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Guevara Morato M. Angeles <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1997<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Pilar Estevez Lopez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jose Castro Perez <\/li>\n<li> Martinez Hernandez M. Jes\u00fas (vocal)<\/li>\n<li>Ignacio Zarra Cameselle (vocal)<\/li>\n<li> Rodriguez Fernandez M. Teresa (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Guevara Morato M. Angeles En esta tesis se han estudiado las enzimas pecticas de fusarium oxysporum f.Sp. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[972,494,35,986,10792,5220,7840],"tags":[248442,2556,6754,70372,4745,4744],"class_list":["post-134341","post","type-post","status-publish","format-standard","hentry","category-botanica","category-ciencias-agrarias","category-ciencias-de-la-vida","category-complutense-de-madrid","category-fitopatologia","category-fitopatologia-agricola","category-hongos","tag-guevara-morato-m-angeles","tag-ignacio-zarra-cameselle","tag-jose-castro-perez","tag-Martinez-hernandez-m-jesus","tag-pilar-estevez-lopez","tag-rodriguez-fernandez-m-teresa"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/134341","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=134341"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/134341\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=134341"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=134341"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=134341"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}