{"id":134384,"date":"1997-01-01T00:00:00","date_gmt":"1997-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/purificacion-y-caracterizacion-de-diferentes-formas-mutantes-de-la-citotoxina-alfa-sarcina\/"},"modified":"1997-01-01T00:00:00","modified_gmt":"1997-01-01T00:00:00","slug":"purificacion-y-caracterizacion-de-diferentes-formas-mutantes-de-la-citotoxina-alfa-sarcina","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/purificacion-y-caracterizacion-de-diferentes-formas-mutantes-de-la-citotoxina-alfa-sarcina\/","title":{"rendered":"Purificacion y caracterizacion de diferentes formas mutantes de la citotoxina alfa-sarcina."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Lacadena Garcia Gallo Francisco Javier <\/strong><\/h2>\n<p>La alfa-sarcina es una proteina producida por el hongo aspergillus giganteus mdh 18894, perteneciente a un grupo de ribotoxinas fungicas que se caracterizan por su caracter citotoxico, debido a su actividad ribonucleolitica. El cdna de esta proteina se ha clonado en diferentes sistemas de expresion permitiendo la purificacion de alfa-sarcina recombinante, estructural y enzimaticamente indistinguible de la fungica. Este sistema de expresion ha permitido la produccion de grandes cantidades de formas marcadas uniforme y selectivamente (tyr y phe) con n15 y c13, aspecto este imprescindible para la resolucion de la estructura tridimensional de la proteina. Se han purificado y caracterizado diferentes formas mutantes de la alfa-sarcina, en residuos pertenecientes al centro activo de la proteina. Esto ha podido confirmar la participacion de las his50 y 137 en el mantenimiento de la actividad ribonucleolitica de esta proteina inactivante de ribosomas.  por otro lado, mutaciones en la cys76 y 132 implican una mayor dificultad en su purificacion y su caracterizacion sugiere el mantenimiento de su capacidad para interaccionar con bicapas lipidicas.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Purificacion y caracterizacion de diferentes formas mutantes de la citotoxina alfa-sarcina.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Purificacion y caracterizacion de diferentes formas mutantes de la citotoxina alfa-sarcina. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Lacadena Garcia Gallo Francisco Javier <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1997<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Alvaro Martinez  Del Pozo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Gregorio Gavilanes Franco <\/li>\n<li> Barbero Esteban Jos\u00e9 Luis (vocal)<\/li>\n<li>Mercedes O\u00f1aderra Sanchez (vocal)<\/li>\n<li>Jos\u00e9 Luis Garcia Lopez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Lacadena Garcia Gallo Francisco Javier La alfa-sarcina es una proteina producida por el hongo aspergillus giganteus mdh [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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