{"id":13869,"date":"2018-03-09T09:00:19","date_gmt":"2018-03-09T09:00:19","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-genetica-y-papel-en-la-patogenesis-de-smcl-una-esfingomielinasa-c-de-listeria-ivanovii\/"},"modified":"2018-03-09T09:00:19","modified_gmt":"2018-03-09T09:00:19","slug":"caracterizacion-genetica-y-papel-en-la-patogenesis-de-smcl-una-esfingomielinasa-c-de-listeria-ivanovii","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/caracterizacion-genetica-y-papel-en-la-patogenesis-de-smcl-una-esfingomielinasa-c-de-listeria-ivanovii\/","title":{"rendered":"Caracterizacion genetica y papel en la patogenesis de smcl, una esfingomielinasa c de listeria ivanovii"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Bruno Gonzalez Zorn <\/strong><\/h2>\n<p>El pat\u00f3geno de rumiantes listeria ivanovii se diferencia de listeria monocytogenes en que produce una fuerte hemolisis bizonal y una caracteristica reaccion de hemolisis cooperativa (tipo camp) en forma de pala con rhodococcus equi. hemos clonado el gen responsable de las propiedades hemol\u00edticas diferenciales de l.Ivanovii,smcl, que codifica una smasa c similar (&gt;50% de identidad) a las esfingomielinasas c de staphylococcus aureus (b-toxina), bacillus cereus y leptospira interrogans. Smcl se transcribe de forma monocistr\u00f3nica y su expresi\u00f3n es independiente de prfa. Southern blots de baja especificidad han demostrado que, dentro del genero listeria, smcl esta presente solamente en l.Ivanovii. Hemos construido un mutante nuloen smcl. Su fenotipo en agar sangre de oveja es identico al de l.Monocytogenes (i.E. Debilmente hemol\u00edtico y sin reaccion tipo camp con r.Equi). Este mutante nulo en smcl. su fenotipo en agar sangre de oveja es identico al de l.Monocytogenes (i.E. debilmente hemol\u00edtico y sin reaccion tipo camp con r.Equi). Este mutante es menos virulento en el modelo murino, y su capacidad de proliferacion intracelular en las celulas epitelioides bovinas mdbk esta disminuida.  se investig\u00f3 el papel de smcl en la supervivencia intracelular utilizando un mutante de l.Monocytogenes que no poseia los determinantes membranol\u00edticos hly, plca y plcb que es, por tanto, incapaz de proliferar en el interior de las celulas. La complementacion de este mutante con smcl en un plasmido fue suficiente para promover su multiplicacion intracelular en las celulas mdbk . Mediante microscopia electronica de trasmisi\u00f3n demostramos que smcl contribuye a la disrupcion de la vacuola fagoc\u00edtica y el escape de la bacteria al citoplasma celular. Por tanto, l.Ivanovii posee una tercera fosfolipasa membranoactiva que, conjuntamente con plca y plcb, podria actuar en concierto con la toxina formadora de poros hly para facilitar el escape de la bacteria de<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizacion genetica y papel en la patogenesis de smcl, una esfingomielinasa c de listeria ivanovii<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizacion genetica y papel en la patogenesis de smcl, una esfingomielinasa c de listeria ivanovii <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Bruno Gonzalez Zorn <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 19\/11\/2001<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Antonio Vazquez  Boland<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: guillermo Suarez fernandez <\/li>\n<li>Francisco Garcia del portillo (vocal)<\/li>\n<li>Fernando Baquero mochales (vocal)<\/li>\n<li>klaus Brehm (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Bruno Gonzalez Zorn El pat\u00f3geno de rumiantes listeria ivanovii se diferencia de listeria monocytogenes en que produce [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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